SB/T 10386-2004 English PDF

US$199.00 · In stock
Delivery: <= 2 days. True-PDF full-copy in English will be manually translated and delivered via email.
SB/T 10386-2004: Determination of chloramphenicol in meat of livestock and poultry
Status: Obsolete

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SB/T 10386-2004	199	Add to Cart	2 days	Determination of chloramphenicol in meat of livestock and poultry	Obsolete

Similar standards

GB 7718   GB/T 5534   GB/T 9695.7   SB/T 11151   SB/T 11092   SB/T 11073   

Basic data

Standard ID: SB/T 10386-2004 (SB/T10386-2004)
Description (Translated English): Determination of chloramphenicol in meat of livestock and poultry
Sector / Industry: Domestic Trade Industry Standard (Recommended)
Classification of Chinese Standard: X04
Classification of International Standard: 67.120
Word Count Estimation: 5,547
Date of Issue: 2004-08-04
Date of Implementation: 2004-09-01
Issuing agency(ies): People Republic of China Ministry of Commerce
Summary: This standard specifies: meat, poultry, chloramphenicol Determination, This standard applies to: meat, poultry, chloramphenicol test.

SB/T 10386-2004: Determination of chloramphenicol in meat of livestock and poultry

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of chloramphenicol in meat of livestock and poultry ICS 67.120 X04 Record number. 14167-2004 People's Republic of China domestic trade industry standard Determination of chloramphenicol in livestock and poultry meat Released on.2004-08-04 2004-09-01 implementation Published by the Ministry of Commerce

Foreword

This standard was proposed by the Ministry of Commerce of the People's Republic This standard was drafted by the Department of Commerce's Slaughter Technology Appraisal Center. The Center for Nutrition and Food Safety of the Chinese Center for Disease Control and Prevention, Hebei Province The Center for Disease Control and Prevention participated in the drafting. The main drafters of this standard. Liu Wenjuan, Yang Zuying, Song Shufeng, Han Huixin, Zhang Xinling, Liu Hucheng, Wang Guiji. This standard is entrusted to the Ministry of Commerce's Slaughter Technology Appraisal Center for interpretation. Determination of chloramphenicol in livestock and poultry meat

1 Scope

This standard specifies the method for determination of chloramphenicol in livestock and poultry. This standard applies to the detection of chloramphenicol in livestock and poultry.

2 Principle

The residual chloramphenicol in the sample was extracted with ethyl acetate, concentrated under reduced pressure, and the residue was dissolved with 0.5 mol/L perchloric acid, and the mixture was treated with n-hexane. In addition to fat, after filtration through a microporous membrane, the filtrate was analyzed by high performance liquid chromatography.

3 reagents and materials

The reagents used below are analytically pure reagents unless otherwise specified, and the water is re-distilled water. 3.1 Methanol. chromatographically pure. 3.2 n-hexane. 3.3 Chloramphenicol is equivalent to 994 units per milligram, content ≥ 99%. 3.4 Ethyl acetate. 3.5 Perchloric acid. 3.6 Chloramphenicol standard stock solution. accurately weigh 0.010g chloramphenicol standard, put it in a 100mL volumetric flask, add methanol to dissolve and dilute To the scale, shake well. The concentration of this solution was 0.10 mg/mL, which was stored in a refrigerator and was valid for 3 months. 3.7 Chloramphenicol standard working solution. Accurately absorb 5.0mL of stock solution in a 50mL volumetric flask and dilute to the mark with methanol. This solution is thick The temperature was 10 μg/mL and stored in the refrigerator. Valid for half a month. Before use, take this liquid and dilute it with 0.5 mol/L perchloric acid to the appropriate concentration. Quasi-working fluid.

4 Instruments and equipment

4.1 High Performance Liquid Chromatograph (with UV detector). 4.2 Rotary evaporator. 4.3 Eddy current mixer. 4.4 Ultrasonic generator. 4.5 Rotate the evaporator. 4.6 Microfiltration membrane filtration.

5 Determination steps

5.1 extraction Take.200g of sample and grind it, weigh 20g (accurate to 0.01g) of the ground sample, place it in a 100mL stoppered flask, and add 40 mL of ethyl acetate, shaken, sonicated for 30 min, filtered, and the filtrate was placed in another flask. Add 20mL of ethyl acetate to try again In the sample, ultrasonic extraction for 15 min, filtration, the filtrate was combined, and the filter residue was further extracted with 20 mL of ethyl acetate for 15 min, filtered, and the filtrate was combined. The filtrate was combined and transferred to a concentrate bottle. 5.2 Purification The combined filtrate was concentrated to dryness on a rotary bath of 52 ° C to 55 ° C. Accurately add 1.0mL0.5mol/L perchloric acid solution The residue in the concentrate bottle was washed, 1 mL of n-hexane was added, shaken for 1 min, and the whole solution was transferred to a 5 mL test tube, and the layer was static. ......

Image     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of SB/T 10386-2004_English be delivered?

Answer: Upon your order, we will start to translate SB/T 10386-2004_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 2 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of SB/T 10386-2004_English with my colleagues?

Answer: Yes. The purchased PDF of SB/T 10386-2004_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.