NY/T 681-2003 English PDF

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NY/T 681-2003: Diagnostic techniques for chicken infectious anemia
Status: Obsolete

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
NY/T 681-2003	519	Add to Cart	3 days	Diagnostic techniques for chicken infectious anemia	Obsolete

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Basic data

Standard ID: NY/T 681-2003 (NY/T681-2003)
Description (Translated English): Diagnostic techniques for chicken infectious anemia
Sector / Industry: Agriculture Industry Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 13,137
Date of Issue: 2003-07-30
Date of Implementation: 2003-10-01
Summary: This standard specifies the chicken infectious anemia (CIA) clinical diagnosis, CIA virus isolation and identification, enzyme-linked immunosorbent assay and enzyme immunoassay testing and other diagnostic techniques. This standard applies to the CIA diagnosis.

NY/T 681-2003: Diagnostic techniques for chicken infectious anemia

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Diagnostic techniques for chicken infectious anemia ICS 11.220 B41 People's Republic of China Agricultural Industry Standard Chicken infectious anemia diagnosis technology Released on July 30,.2003 Implementation of.2003-10-01 Published by the Ministry of Agriculture

Foreword

Appendix A of this standard is a normative appendix. This standard is proposed and managed by the Animal Husbandry and Veterinary Bureau of the Ministry of Agriculture. This standard was drafted. Veterinary Diagnostic Center of the Ministry of Agriculture. The main drafters of this standard. Wang Hongwei, Wu Qingmin, Tian Kegong, Chen Xiwei, Su Jingliang, Wang Chuanbin. Chicken infectious anemia diagnosis technology

1 Scope

This standard specifies the clinical diagnosis of chicken infectious anemia (CIA), the isolation and identification of CIA virus, enzyme-linked immunosorbent assay and immunization Diagnostic techniques such as enzyme testing. This standard applies to the diagnosis of CIA.

2 clinical diagnosis

2.1 Clinical symptoms 2.1.1 The main clinical feature of CIA is anemia. The sick chickens showed signs of weakness, weakness, accumulation and slow movements, rough feathers, sputum, meat, Facial and visible mucous membranes are pale, there are bleeding lesions in the feathers, poor growth, and white and thin feces. 2.1.2 Egg-laying breeder infection has no clinical symptoms, and the egg production rate, fertilization rate and hatching rate are not affected, but the virus can be transmitted by breeding eggs. 2.1.3 Chicken infectious anemia virus when there is an immunosuppressive disease such as Marek's disease or infectious bursal disease The susceptibility of (CIAV) is increased, and the onset is early and severe. 2.2 pathological changes 2.2.1 sick chickens show aplastic anemia, weight loss, white bone marrow, pale muscles and internal organs, liver and kidney swelling and fading, blood Thin, prolonged clotting time. 2.2.2 diseased chickens show systemic lymphoid tissue atrophy, thymus, bursa, spleen and cecum, tonsils and other tissues within the lymphocytes Heavy missing.

3 Virus isolation and identification

3.1 Material preparation 3.1.1 Reagents a) Improve the minimum element nutrient solution (DMEM) medium, see formula A. Chapter 1; b) Marek's disease virus-transformed lymphocyte lineage MDCC-MSB1 cells; c) standard positive serum. serum obtained by immunizing SPF chicken with CIAV antigen; d) standard negative serum. SPF chicken serum without CIAV infection; e) Newborn bovine serum, penicillin, streptomycin. 3.1.2 Equipment a) cell culture flask; b) a straw; c) a carbon dioxide incubator; d) 37 ° C constant temperature water bath; e) ordinary refrigerators and low temperature refrigerators; f) centrifuges and centrifuge tubes; g) grinding equipment; h) ordinary optical microscope; i) micro-sampler, capacity 5μL ~ 50μL, 50μL ~.200μL; j) 0.45 μm microporous membrane. 3.1.3 Sample ......

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