LY/T 3112-2019 English PDFUS$419.00 · In stock
Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email. LY/T 3112-2019: Technical regulation of fox artificial insemination Status: Valid
Basic dataStandard ID: LY/T 3112-2019 (LY/T3112-2019)Description (Translated English): Technical regulation of fox artificial insemination Sector / Industry: Forestry Industry Standard (Recommended) Classification of Chinese Standard: B44 Classification of International Standard: 65.020.30 Word Count Estimation: 18,138 Date of Issue: 2019-10-23 Date of Implementation: 2020-04-01 Issuing agency(ies): State Forestry and Grassland Administration LY/T 3112-2019: Technical regulation of fox artificial insemination---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.(Fox artificial insemination technical regulations) ICS 65.020.30 B 44 LY People's Republic of China Forestry Industry Standard Fox artificial insemination technical regulations Technical regulation of fox artificial insemination 2019-10-23 released 2020-04-01 implementation Published by the National Forestry and Grassland Administration ContentsForeword ... II 1 Scope ... 1 2 Terms and definitions ... 1 3 Preparation before insemination ... 2 4 Personnel requirements ... 3 5 fox requirements ... 3 6 Insemination operation ... 4 7 Management after insemination ... 7 Appendix A (informative appendix) List of commonly used equipment and drugs for artificial insemination ... 8 Appendix B (informational appendix) Semen quality assessment methods ... 9 Appendix C (informative) Estrus evaluation ... 11 Appendix D (Informative) Commonly Used Record Form for Artificial Insemination ... 12ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed and managed by the National Technical Committee for Wildlife Conservation, Management, Operation and Utilization (SAC/TC369). This standard was drafted. Harbin Institute of Specialty Products, Heilongjiang Academy of Agricultural Reclamation Sciences, Heilongjiang Pratt & Whitney Specialty Products Co., Ltd. The main drafters of this standard. Han Huansheng, Zhao Liping, Liu Zhiping, Wei Ximing, Yin Yajie, Shi Wenqing, Xu Xin, An Rui, Zhai Xuechao, Yang Yang, Yang Jiao, Zhao Xiaojing, Chai Menglong, Zhang Haiyan Fox artificial insemination technical regulations1 ScopeThis standard specifies the technical requirements for artificial insemination of blue foxes and silver black foxes. Other foxes can be used in reference. This standard applies to breeding of artificially-bred foxes in breeding farms and artificial insemination stations.2 terms and definitionsThe following terms and definitions apply to this document. 2.1 Fresh Semen Diluent Formulated with diluent, nutrient and additives as main ingredients, suitable for fresh sperm that can be fertilized when stored at 33 ℃ ～ 37 ℃ for 3h Solution. 2.2 Sperm motility The percentage of sperm in the sperm that moves in a straight line in the semen, commonly known as the sperm motility rate. 2.3 Sperm concentration The total number of sperm contained in a unit volume of semen is also called the sperm concentration. 2.4 Sperm deformity rate Percentage of spermatozoa with morphological variation of head, body, and tail in a unit volume of semen. 2.5 Number of effective sperms The total number of spermatozoa in a normal sperm motion in the semen sample tested, that is, the number of spermatozoa with fertility. 2.6 External observation methods of estrus identification A method for judging whether estrus and estrus are in accordance with the characteristic behavior, mental state, and vulvar changes of the female fox, referred to as external view Chafa. 2.7 Artificial insemination An animal breeding technique that uses auxiliary equipment to artificially deliver semen into the uterus of an estrus female fox to conceive it.3 Preparation before insemination3.1 Planning Before artificial insemination begins, a plan should be prepared, pre-selected supplies (see Appendix A), and design record sheets (see D.1 to D.3 in Appendix D). 3.2 Insemination operation room 3.2.1 The operation room should be located near the wind direction of the fox farm, with an area of 15m2 ~ 25m2, which requires safety, ventilation, heat insulation and sanitation. Lighting, electrical outlets and consoles. 3.2.2 The operation room shall be divided into a dressing room, a sperm collection and insemination room, and a semen detection and processing room, with a partition in the middle. 3.2.3 The wall and floor of the operating room shall be made of ceramic or hard waterproof materials which are easy to disinfect. 3.2.4 The operating room should be equipped with ultraviolet disinfection lamps and temperature control equipment, and the temperature should be controlled at 18 ℃ ～ 25 ℃. 3.3 supplies preparation 3.3.1 Prepare medicines and equipment before insemination (see Appendix A). 3.3.2 The dressing room shall be equipped with clothes hangers, shoe racks, sinks or sinks. 3.3.3 The sperm collection and insemination room should be equipped with Baoding stand, water basin, towels, washing and disinfecting drugs, vaginal dilation tube, insemination needle, 1mL ～ 10mL Injectors, scissors, waste collection bins, etc. 3.3.4 The semen detection and processing room shall be equipped with an operating table, a collection cup, a thermometer, a microscope, a glass slide, a cover glass, a dropper, and a thermostatic water bath Pots and washing and disinfecting supplies. 3.3.5 Estrus identification should be equipped with alcohol cotton balls, cotton swabs, microscopes, glass slides, etc., and resistance testers should be equipped. 3.3.6 The environment and equipment cleaning and disinfection shall be equipped with test tube brushes, continuous syringes, distilled water, washing and disinfecting drugs, drying boxes, disinfection cabinets and Autoclaves, etc. 3.4 Cleaning and disinfection 3.4.1 Environment For the first disinfection before the start of artificial insemination, it is advisable to use fire alkali or quicklime for disinfection outside the operating room and 1% to 4% sodium hydroxide or 0.1% to indoor 0.2% Xin Jie Er disinfection solution, in the future indoors should be disinfected with ultraviolet light every day, should not use odor disinfection drugs. 3.4.2 Equipment 3.4.2.1 The artificial insemination equipment should be selectively cleaned and disinfected according to the material. 3.4.2.2 Wash before disinfection. Various equipment and utensils should be cleaned and disinfected before and after use. Wash with water and detergent before cleaning. Dirt is then rinsed with water. Utensils in contact with semen should be rinsed with distilled water at least twice before drying. 3.4.2.3 After the metal and glass utensils are soaked and brushed with disinfectant, they are dried in a 120 ° C drying oven for more than 2 hours. 3.4.2.4 Plastic products (such as plastic vaginal dilatation tubes) can be washed in accordance with the above procedure, then immersed in 75% alcohol for 1 to 2 hours, and then distilled Wash with water, and then dry in a drying box at 60 ℃ ～ 80 ℃ according to the material. 3.4.2.5 Towels and work clothes used for wiping the fox's genital area can be sterilized by high-pressure steam sterilizer after washing. 3.4.3 personnel Operators should strictly disinfect before and after artificial insemination. The sperm collector should cut his nails, wash his hands with soapy water, and rinse with water. Wash and dry, wear disposable gloves, and work clothes.4 Staff requirements4.1 The staff consists of estrus appraisers, Baoding staff, insemination assistants and insemination staff, and no zoonotic disease is required. 4.2 One or two estrus appraisers. The number of personnel should be determined according to the number of female foxes and their working capabilities. It is necessary to train the breeder to make a preliminary determination of the estrus status of the female fox under his control. 4.3 One Baoding staff member, who is a full-time Baoding fox for sperm collection and insemination in the operating room. 4.4 One insemination assistant assists the insemination worker, and is also responsible for the scrubbing and disinfection of the equipment, indoor hygiene, and records of semen collection and insemination. 4.5 One insemination worker, with certain professional theoretical foundation and field work experience.5 fox requirements5.1 male foxes Male foxes that can be harvested and bred should meet the following conditions. a) Have the characteristics and breeding requirements of this variety; b) entering the body condition during mating period; c) over 10 months of age; d) Pure fur color and clear genealogy; e) Healthy and non-infectious diseases, mainly refers to no deformity of testis, no adhesion of foreskin, no inflammation of reproductive system, no Brucella and Gard Infectious diseases f) Reproductive performance requirements. The semen quality should be tested every two weeks during estrus. The quality of fresh essence should meet the requirements of 6.3 of this standard. 5.2 Female Foxes Breeding female foxes should meet the following conditions. a) Have the characteristics and breeding requirements of this variety; b) entering into the mating period with moderate body condition; c) over 10 months old and under 6 years old; d) Pure fur color and clear genealogy; e) Healthy and non-infectious diseases, mainly refers to the absence of malformations and inflammation in the reproductive system, and no infectious diseases such as Brucella and Gardnerella; f) Reproductive performance requirements. strong motherhood, no vacancy last year, and more litters.6 Insemination operation6.1 Estrus evaluation 6.1.1 The estrus identification can be based on the observation of the vulva of the female fox, the estrus identification of the vaginal cells, or the comprehensive determination method supplemented by the resistance tester. Method (see Appendix C). 6.1.2 In the estrus period, the estrus appraiser performs vulva observation records on the mating female foxes, and regularly observes female foxes with estrus performance, and finally The time and number of inseminations were determined by vaginal cell estrus identification method or resistance tester, and the number of female foxes that could inseminate each day was reported to the insemination staff. 6.1.3 The best time for insemination is female fox vulva swelling and retraction, wrinkles appear, vaginal cell smears have no more than 80% of keratinocytes, resistance measurement The resistance value of the meter is changed from high to low. 6.2 Semen collection 6.2.1 Preparation before harvesting The following preparations should be made before semen collection. a) Prepare supplies according to the number of female inseminations per day; b) Put the sterilized insemination needle, sperm collection cup, and vaginal dilation tube into a 33 ℃ ～ 37 ℃ incubator; c) Wet the sterilized towel with 0.1% ～ 0.2% Xinjieer sterilized warm solution; d) Preheating the fresh essence diluent in a constant temperature water bath ranging from 33 ° C to 37 ° C for 30 minutes in advance; e) Determine the number of male foxes. 6.2.2 Baoding Put the sperm-extracting male fox on the Baoding rack, and the Baoding members fix the head of the fox with neck clamps or clamps, and at the same time hold the tail to make the male fox stand relaxed Potential. 6.2.3 Cleaning and disinfection Wipe the abdomen and scrotum of the collected foxes with a disinfected warm and damp towel. The plush is wet and free of dust and dust. 6.2.4 Extraction operation 6.2.4.1 During sperm harvesting, first massage the testicles and penis of the male fox to give the fox a signal of sperm harvesting. 6.2.4.2 After massaging for a few seconds, the thumb and index finger of the sperm collector are pinched on both sides of the male fox penis, the middle finger is pinched on the ventral surface of the penis, and Press and slide the penis foreskin longitudinally along the penis to rub the penis with friction. 6.2.4.3 The sliding speed should be faster at the beginning of pressing, preferably 4 times/s to 5 times/s, and the moving range should be 7cm ～ 8cm. After pressing for 5s ～ 7s, The penis is erect, and the corpus cavernosum in the middle of the penis also swells. 6.2.4.4 Then, pull the penis from the rear legs of the male fox to the back, push the foreskin to the back of the corpus cavernosum and continue to squeeze the corpus cavernosum And the posterior penis, the pressure of the pressure is slowed down, it is advisable to pressure 12 to 13 times per 10s, and the pressure is slightly harder when pressing the spherical sponge, so repeat Pressure massage (about 10s) until the male fox ejaculates. 6.2.5 Semen collection Only semi-milk semen was collected during semen collection. 6.2.6 Semen collection frequency In each estrus season, each male fox can collect sperm from 15 to 25 times, and the frequency of sperm collecting should not exceed 2 times in 3 days. 6.3 Semen test The collected semen should be tested and recorded (see Table D.1 in Appendix B and Appendix D). The quality of fresh sperm should meet. a) The color of fresh essence is milky white or slightly yellow; b) no urine, blood and dirt; c) semen volume ≥0.3mL; d) sperm motility ≥ 0.7; e) sperm deformity rate ≤15%; f) Sperm density ≥400 million/mL. 6.4 Semen dilution 6.4.1 Dilution multiple Place the sperm collection cup into the thermostatic water bath of 33 ℃ ～ 37 ℃, and determine the dilution according to the sperm motility, sperm density and semen volume. multiple. 6.4.2 Isothermal Dilution Use isothermal fresh sperm diluent for stepwise dilution. The method is to slowly add the diluent to the semen along the wall of the sperm collection cup, and gently mix it. It is strictly prohibited. Quickly flush the diluted solution into the semen and shake vigorously to ensure that there is at least 60 million effective sperm per mL in the diluted semen. Medium density is better (see Appendix B). 6.5 Semen preservation 6.5.1 Semen should be infused immediately after the semen is diluted. The semen should not be stored for more than 3 hours in a 33 ℃ ～ 37 ℃ water bath. 6.5.2 If the semen needs to be postponed, it should be diluted with 15% -20% egg yolk fresh sperm dilution solution, and then stored in a refrigerator at 4 ℃ ～ 6 ℃. 2 After the day is used up, the temperature of the microscope heating plate should be controlled at 35 ℃ ～ 37 ℃ before use. 6.6 Artificial insemination 6.6.1 Female Fox Baoding Grab the female fox to be inseminated, fix it on the Baoding rack, and place the female fox upside down or stand in Baoding. 6.6.2 Cleaning and disinfection Wipe the vulva and its surroundings with a sterile warm and damp towel. 6.6.3 Insemination operation 6.6.3.1 Slowly insert the vaginal dilatation tube into the vagina of the female fox, and its front end reaches the cervix. The left mouth of the tiger is supported on the lower abdomen of the female fox. Fingers, index and middle fingers touch the front of the vaginal dilatation tube. 6.6.3.2 The cervix is fixed in the left hand, and the insemination needle is gently inserted through the lumen of the vaginal dilatation tube in the right hand, with the front end touching the cervix. 6.6.3.3 Search the cervix with your left and right hands. After the insemination needle passes through the cervix, it will be fixed at a position of 1cm to 2cm in the uterus. 6.6.3.4 The assistant inserts the syringe that sucks semen on the insemination needle, and pushes the syringe to slowly inject the semen into the uterus. A semen-suctioning syringe is inserted into the insemination needle in advance, and the inseminater directly injects the semen. 6.6.3.5 When sucking semen with a syringe, after sucking to a fixed scale, a little more air should be sucked in to ensure that all semen is infused during insemination Into the uterus. 6.6.4 Dose for insemination Each female fox should have an insemination dose of 0.8 mL to 1.0 mL. 6.6.5 Frequency of insemination It is advisable for each female fox to lose 2 to 3 times during each estrus. It can be inseminated every other day, or it can be stopped for one day and then lost again. 6.6.6 Work after insemination The following tasks should be performed after the artificial insemination. a) Clean and disinfect the operation room and destroy the waste products; b) Clean and disinfect the instruments in the same way as 3.4.2, and store the equipment uniformly; c) Organize the records and arrange the next insemination plan.7 Management after insemination7.1 Insemination fox 7.1.1 Infertile female foxes should avoid fright, to prevent abnormal fetal position, abortion and dystocia. 7.1.2 The feed of inseminated female foxes should not be greatly changed, and must not be fed with moldy, icy feeds. 7.1.3 Make relevant records of infertile female fox disease, physical condition, and abortion (see Table D.2 in Appendix D). 7.1.4 Before entering the pre-birth period, do prenatal preparations, increase staff, and make preparations for fox rearing, feeding, delivery, and disease prevention. Make. 7.2 Birth records Keep farrowing records (see Table D.2 in Appendix D) and archive them. 7.3 Work summary Summarize the overall work of artificial insemination (see Table D.3 in Appendix D), summarize, and archive. AAAppendix A(Informative appendix) Table of commonly used equipment for artificial insemination A.1 List of commonly used equipment for artificial insemination No. Product Name Unit Quantity Specification 1 Drying box table 1 2 Disinfection counter 1 3 Refrigerator counter 1 4 Constant temperature water bath pot table 1 5 Steam sterilization pot table 1 6 Alcohol bottles with 75% alcohol7 New Gil disinfectant bottle amount8 Distilled water bucket9 appropriate amount of washing liquid bottle10 amount of sink 11 Towel blocks 12 test tubes 13 continuous syringes 2 ～ 3 15 sets of fine cups 16 insemination needles 17 vaginal dilatation tubes 18 syringes 1mL, 2/2.5mL, 5mL, 10mL 19 microscope stage 1 400x ～ 600x 20 constant temperature heating plate 1 21 Coverslips/Slide Boxes 22 wipe the right amount on paper 23 cotton swabs 24 appropriate amount of disposable gloves/mask pack 25 Scissors Pack 1 26 Appropriate amount of work clothes 27 Ultrasonic cleaner table 1 28 Distilled water table 1 29 Air Conditioner 1 30 UV lamp ≥2W/m2 appropriate amount Sperm density meter/cytometer Number board Desk/piece 1 BBAppendix B(Informative appendix) Semen quality assessment method B.1 Sperm motility (or sperm motility) B.1.1 Sperm viability should be assessed by microscopy; B.1.2 Drop a drop of semen on an ordinary glass slide, and then cover the entire liquid surface with a cover glass to make a microscopic examination of the tablet; B.1.3 Perform visual evaluation under a microscope with a constant temperature heating plate (33 ° C to 37 ° C) at 400 times to 600 times; B.1.4 Use a 10-point scoring standard from 0 to 1.0; B.1.5 Estimate the percentage of spermatozoa moving forward in the semen sample; B.1.6 100% for straight forward athletes is 1.0 points, that is, sperm vitality is 1.0, 90% for straight forward athletes is 0.9 points, that is, sperm vitality 0.9, and so on. B.2 Sperm density B.2.1 Sperm density should be detected by blood cell counting method; B.2.2 Pipette 0.2mL or 2mL of 3% NaCl solution into a small test tube with a 1mL thin tube; B.2.3 According to the requirement of the dilution factor, use a blood pipette to aspirate and discard 10µL or 20µL of 3% NaCl solution (or diluent); B.2.4 Use a blood pipette to aspirate 10 µL or 20 µL of the semen to be tested and inject it into a small test tube and shake well; B.2.5 Take a drop of diluted semen on the edge of the cover glass on the calculation board to allow the semen to penetrate into the calculation chamber and fill it up. With bubbles B.2.6 Sperm in 80 small squares in th......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of LY/T 3112-2019_English be delivered?Answer: Upon your order, we will start to translate LY/T 3112-2019_English as soon as possible, and keep you informed of the progress. The lead time is typically 2 ~ 4 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of LY/T 3112-2019_English with my colleagues?Answer: Yes. The purchased PDF of LY/T 3112-2019_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay. |
