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LY/T 2620-2016 English PDF

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LY/T 2620-2016: Technical regulation for Betula alnoides clones prapagation via tissue culture
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
LY/T 2620-2016189 Add to Cart 3 days Technical regulation for Betula alnoides clones prapagation via tissue culture Valid

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Basic data

Standard ID: LY/T 2620-2016 (LY/T2620-2016)
Description (Translated English): Technical regulation for Betula alnoides clones prapagation via tissue culture
Sector / Industry: Forestry Industry Standard (Recommended)
Classification of Chinese Standard: B61
Classification of International Standard: 65.020.20
Word Count Estimation: 8,831
Date of Issue: 2016-01-18
Date of Implementation: 2016-06-01
Quoted Standard: GB/T 6001; LY/T 1000; LY/T 1882; LY/T 1948
Regulation (derived from): ?State Forestry Bureau Announcement 2016 No.1
Issuing agency(ies): State Forestry Administration
Summary: This standard prescribes the technical requirements for explant collection and treatment of tissue culture seedlings of Betula alnoides, preparation of culture medium, propagation and rooting of plantlets, transplanting of plantlets, management of nursery stock and nursery. This standard applies to tissue culture and propagation of Betula alnoides clones "Qingshan 1", "Qingshan 2", "Qingshan 3", "Qingshan 4" and "Qingshan 5".

LY/T 2620-2016: Technical regulation for Betula alnoides clones prapagation via tissue culture

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Technical regulation for Betula alnoides clones prapagation via tissue culture ICS 65.020.20 B61 People's Republic of China Forestry Industry Standard Technical regulations for breeding seedlings of Betula platyphylla Released on.2016-01-18 2016-06-01 implementation State Forestry Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is under the jurisdiction of the National Forest Seed Standardization Technical Committee (SAC/TC115). This standard is drafted by. Tropical Forestry Experiment Center of Chinese Academy of Forestry. The main drafters of this standard. Yan Honghui, Cai Daoxiong, Jia Hongyan, Wang Xiaoning, Guo Wenfu, Liu Zhilong, Nong Shuxia, Meng Cailan, Ma Yue. Technical regulations for breeding seedlings of Betula platyphylla

1 Scope

This standard stipulates the collection and treatment of explants from the growth of Betula platyphylla clones, medium preparation, tissue culture seedling proliferation and rooting culture, Technical requirements for transplanting seedlings, seedling management and hoeing. This standard applies to the tissue culture of the southwestern birch clones "Qingshan No.1", "Qingshan No.2", "Qingshan No.3", "Qingshan No.4" and "Qingshan No.5" Fast and complicated.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6001 nursery technical regulations LY/T 1000 container seedling technology LY/T 1882 Technical regulations for tissue culture and seedling cultivation LY/T 1948 Technical Regulations for Cultivation of Southwestern Birch Forest

3 Terms and definitions

The terms and definitions defined by LY/T 1882 apply to this document.

4 medium preparation

4.1 Medium composition Explant induction medium, proliferation medium and rooting medium are shown in Appendix A. 4.2 Mother liquor preparation Follow LY/T 1882. 4.3 Medium preparation 4.3.1 According to the formulation of the medium and the amount of the culture medium, the mother liquor is prepared by proportionately, and the medium solution is stirred evenly. Then add the second mother liquor, and add a certain amount of coagulant and sucrose, add water to volume, and stir and dissolve. 4.3.2 Heat the prepared medium solution to boiling in a stainless steel pot to completely dissolve the medium, then use 1.0mol/L hydrochloric acid or The pH was adjusted to 5.8 with 1.0 mol/L sodium hydroxide. 4.3.3 The medium is heated and dissolved, and then quantitatively dispensed into a light-transmissive glass culture bottle with a height of 10 cm to 15 cm for sealing. 4.4 Disinfection of the medium 4.4.1 Disinfect the dispensed medium in a sterile pot. The disinfection process is strictly in accordance with the technical regulations for the use of the sterilization pot, and the sterilization pressure 0.11 MPa is used, the temperature is 121 ° C, and the disinfection time is 25 min to 30 min.
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