LST6138-2020 English PDF

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LST6138-2020: Inspection of grain and oils--Determination of aflatoxins in grains and oils--Ultra high liquid chromatography with immunomagnetic bead clean-up
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
LS/T 6138-2020	209	Add to Cart	3 days	Inspection of grain and oils--Determination of aflatoxins in grains and oils--Ultra high liquid chromatography with immunomagnetic bead clean-up	Valid

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Basic data

Standard ID: LS/T 6138-2020 (LS/T6138-2020)
Description (Translated English): Inspection of grain and oils--Determination of aflatoxins in grains and oils--Ultra high liquid chromatography with immunomagnetic bead clean-up
Sector / Industry: Food & Beverage Industry Standard (Recommended)
Classification of Chinese Standard: X11
Classification of International Standard: 67.040
Word Count Estimation: 9,944
Date of Issue: 2020
Date of Implementation: 2020-07-21
Issuing agency(ies): National Grain and Material Reserves Administration

LST6138-2020: Inspection of grain and oils--Determination of aflatoxins in grains and oils--Ultra high liquid chromatography with immunomagnetic bead clean-up

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Inspection of grain and oils--Determination of aflatoxins in grains and oils--Ultra high liquid chromatography with immunomagnetic bead clean-up ICS 67.040 X11 People's Republic of China Food Industry Standard Grain and oil inspection Determination of aflatoxin in grain Immune magnetic beads purification ultra high performance liquid chromatography 2020-01-21 release 2020-07-21 implementation Issued by the National Grain and Material Reserve Bureau

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the National Grain and Material Reserve Bureau. This standard is under the jurisdiction of the National Grain and Oil Standardization Technical Committee (SAT/TC270). This standard was drafted by. Scientific Research Institute of the State Food and Material Reserve Bureau, Standard Quality Center of the State Food and Material Reserve Bureau, Beijing Dongfu Jiuheng Instrument Technology Co., Ltd., China National Grain Management Group Co., Ltd., Shandong Grain and Oil Testing Center, Guangxi Zhuang Autonomous Region Grain Oil Quality Inspection Center, Sichuan Grain and Oil Center Monitoring Station, Henan Port Food Inspection Center, Suining Grain Quality Supervision and Inspection Station, Anhui Province Grain and Oil Product Quality Supervision and Inspection Station, Henan Grain, Oil and Feed Product Quality Supervision and Inspection Center, Fujian Grain and Oil Quality Monitoring Institute, Hubei Grain Oil and Food Quality Supervision and Inspection Center, Liaoning Grain and Oil Inspection and Monitoring Institute, Heilongjiang Grain Quality and Safety Monitoring Center, Shanxi Grain Quality Supervision Testing Center, Yunnan Grain and Oil Products Quality Supervision, Inspection and Testing Center, Inner Mongolia Autonomous Region Grain and Oil Quality Testing Center, Kunming Grain, Oil and Feed Production Quality Inspection Center, Beijing Agricultural Quality Standards and Testing Technology Research Center, Harbin Grain Quality Monitoring Station, Qianguoluosi Mongolia Minority Autonomous County Grain and Oil Quality Sanitation Inspection and Monitoring Station. The main drafters of this standard. Wang Songxue, Ye Jin, Xuan Zhihong, Yang Weimin, Li Keqiang, Zhang Zhihang, Zhou Minghui, Wang Pei, Wu Xianshao, Yang Jun, Ji Jiansheng, Li Xiaoming, Hu Bin, Liu Ying, Xie Tingting, Tian Guojun, Han Xiao, Xu Chunfeng, Li Jing, Shao Zhiling, Qiu Qingfeng, Li Weixiang, Lu Anxiang, Zhao Hongwei and Zhao Huanyu. Grain and oil inspection Determination of aflatoxin in grain Immune magnetic beads purification ultra high performance liquid chromatography

1 Scope

This standard specifies the determination of aflatoxins B1, B2, G1, G2 (AFB1, AFB2, Principles of AFG1, AFG2), reagents and materials, instruments and equipment, analysis steps, determination, result calculation and precision, etc. This standard is applicable to the determination of AFB1, AFB2, AFG1 and AFG2 in grain, oil and other samples of wheat, corn, rice and vegetable oil. The detection limit of this standard AFB1 is 0.1 μg/kg, the detection limit of AFB2 is 0.03 μg/kg, and the detection limit of AGF1 is 0.1 μg/kg, The detection limit of AFG2 is 0.03 μg/kg; the limit of quantification of AFB1 is 0.3 μg/kg, the limit of quantification of AFB2 is 0.1 μg/kg, and the quantification of AFG1 The limit is 0.3 μg/kg, and the limit of quantification of AFG2 is 0.1 μg/kg.

2 Normative references

The following documents are essential for the application of this document. For dated references, only the dated version applies to this article Pieces. For the cited documents without date, the latest version (including all amendments) applies to this document. GB/T 5490 General Rules for Grain and Oil Inspection GB/T 5491 Grain and Oil Inspection Sampling and Sampling Method GB/T 6682 Analysis laboratory water specifications and test methods

3 Principle

The aflatoxin in the sample was extracted with methanol-water mixed solution, the supernatant was purified and enriched by immunomagnetic beads, and then separated by ultra-high performance liquid chromatography Analysis and quantification by external standard method.

4 Reagents and materials

Unless otherwise stated, the reagents used in this method are analytically pure, and the experimental water should meet the first-grade water requirements specified in GB/T 6682. 4.1 Acetonitrile. chromatographically pure. 4.2 Methanol. chromatographically pure. 4.3 Sodium chloride. 4.4 Disodium hydrogen phosphate. 4.5 Potassium dihydrogen phosphate. 4.6 Potassium chloride. 4.7 Hydrochloric acid. 4.8 TritonX-100 [C14H22O (C2H4O) n] (or Tween-20, C58H114O26). 4.9 Methanol-water mixture 70.30, volume fraction 70mL methanol was added to 30mL water and mixed. 4.10 Phosphate buffer solution (hereinafter referred to as PBS). Weigh 8.00g sodium chloride, 1.20g disodium hydrogen phosphate (or 2.92g phosphoric acid dodecahydrate) Disodium hydrogen), 0.20g potassium dihydrogen phosphate, 0.20g potassium chloride, dissolved in 900mL of water, adjust the pH to 7.4 with hydrochloric acid, and make up to volume with water 1000mL. ......

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