LST6128-2017 English PDF

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LST6128-2017: Inspection of grain and oils - Determination of aflatoxin B1, B2, G1, G2 in grains - Ultra-high performance liquid chromatography
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
LS/T 6128-2017	229	Add to Cart	3 days	Inspection of grain and oils - Determination of aflatoxin B1, B2, G1, G2 in grains - Ultra-high performance liquid chromatography	Valid

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Basic data

Standard ID: LS/T 6128-2017 (LS/T6128-2017)
Description (Translated English): Inspection of grain and oils - Determination of aflatoxin B1, B2, G1, G2 in grains - Ultra-high performance liquid chromatography
Sector / Industry: Food & Beverage Industry Standard (Recommended)
Classification of Chinese Standard: X04
Classification of International Standard: 67.040
Word Count Estimation: 10,138
Date of Issue: 2017-10-27
Date of Implementation: 2017-12-20
Regulation (derived from): State-Grain-Communication (2017) 6
Issuing agency(ies): National Grain Administration

LST6128-2017: Inspection of grain and oils - Determination of aflatoxin B1, B2, G1, G2 in grains - Ultra-high performance liquid chromatography

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Inspection of grain and oils-Determination of aflatoxin B1, B2, G1, G2 in grains-Ultra-high performance liquid chromatography ICS 67.040 X04 People's Republic of China food industry standard Grain and oil testing aflatoxins B1, B2 in food Determination of G1 and G2 by ultra performance liquid chromatography Inspectionofgrainandoils-DeterminationofaflatoxinB1, B2, G1, G2ingrains-Ultra-highperformanceliquidchromatography Released on.2017-10-27 2017-12-20 implementation National Food Bureau released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the National Grain Administration. This standard is under the jurisdiction of the National Grain and Oil Standardization Technical Committee (SAT/TC270). This standard was drafted. National Academy of Food Science Research Institute, Hunan National Grain Quality Monitoring Center, Jilin Province Grain and Oil Hygiene Inspection and Monitoring Station, Heilongjiang Grain and Oil Hygiene Inspection and Monitoring Station, Beijing Agricultural Quality Standards and Testing Technology Research Center. The main drafters of this standard. Xie Gang, Wang Songxue, Li Li, Li Rui, Ye Jin, Wang Yuanyuan, Wu Yu, Xin Yuanyuan, Ni Xiaoying, Shi Jiayuan, Luo Yan, Lu Anxiang. Grain and oil testing aflatoxins B1, B2 in food Determination of G1 and G2 by ultra performance liquid chromatography

1 Scope

This standard specifies the principle, reagents and instruments for the determination of aflatoxins B1, B2, G1 and G2 in food and its products by ultra performance liquid chromatography. Equipment, analysis steps, results calculations, etc. This standard applies to the determination of aflatoxins B1, B2, G1, G2 in food and its products. The detection limit of aflatoxin B1 in this standard method is 0.2μg/kg, the limit of quantification is 0.4μg/kg; the detection limit of aflatoxin G1 is 0.5μg/kg, the limit of quantification was 1.5μg/kg; the detection limit of aflatoxin B2 and G2 was 0.1μg/kg, and the limit of quantification was 0.3μg/kg.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. Preparation of standard solution for GB/T 602 chemical reagent impurity determination GB/T 5491 Inspection of food and oil materials, sample method GB/T 6682 Analytical laboratory water specifications and test methods

3 Principle

The aflatoxins B1, B2, G1, G2 in the sample are extracted with the extract, and the immunoaffinity column is purified and enriched, and then passed through a large flow cell. The measurement was carried out by an ultra performance liquid chromatography method and quantified by an external standard method.

4 reagents and equipment

Unless otherwise specified, the reagents used are of analytical grade, and the experimental water should meet the first-class water requirements of GB/T 6682. 4.1 Reagents 4.1.1 Methanol (CH3OH). chromatographically pure. 4.1.2 Acetonitrile (CH3CN). chromatographically pure. 4.1.3 Sodium chloride (NaCl). analytically pure. 4.1.4 Aqueous methanol solution. Measure 70 volumes of methanol (4.1.1), add 30 volumes of water, and mix. Other reagents meet the requirements of GB/T 602. 4.2 Standards 4.2.1 Aflatoxin standard (aflatoxin B1, B2, G1, G2). purity ≥ 99%; or certified by the state and granted standard substance certificate The standard substance of the book. 4.2.2 Standard stock solution. aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), Aflatoxin G2 (AFG2) standard (4.2.1) each 0.1mg (accurate to 0.01mg), dissolved with methanol (4.1.1) and made up to 10mL, ......

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