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Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. LST6124-2017: Inspection of grain and oils - Determination of polyphenol oxidase activity in wheat flour - Spectro-photometric method Status: Valid
Basic dataStandard ID: LS/T 6124-2017 (LS/T6124-2017)Description (Translated English): Inspection of grain and oils - Determination of polyphenol oxidase activity in wheat flour - Spectro-photometric method Sector / Industry: Food & Beverage Industry Standard (Recommended) Classification of Chinese Standard: X11 Classification of International Standard: 67.060 Word Count Estimation: 6,667 Date of Issue: 2017-10-27 Date of Implementation: 2017-12-20 Issuing agency(ies): National Grain Administration LST6124-2017: Inspection of grain and oils - Determination of polyphenol oxidase activity in wheat flour - Spectro-photometric method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Inspection of grain and oils-Determination of polyphenol oxidase activity in wheat flour-Spectro-photometric method ICS 67.060 X11 People's Republic of China food industry standard Determination of Polyphenol Oxidase Activity in Wheat Flour by Grain and Oil Released on.2017-10-27 2017-12-20 implementation National Food Bureau released ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the National Grain Administration. This standard is under the jurisdiction of the National Grain and Oil Standardization Technical Committee (SAC/TC270). This standard was drafted. Henan University of Technology, National Food Bureau Standard Quality Center, Henan Province Grain and Oilstuff Product Quality Supervision and Inspection Station, Anhui Provincial Grain and Oil Product Quality Supervision and Inspection Station, Shaanxi Provincial Grain and Oil Product Quality Supervision and Inspection Institute, Hubei Grain and Oil Food Quality Monitoring Station, Shanxi Food Quality Monitoring Center, Guangdong Product Quality Supervision and Inspection Institute, Guangdong National Grain Quality Monitoring Center. The main drafters of this standard. Chen Jie, Yan Ke, Lu Yingguo, Zhu Zhiguang, Li Xueqin, Wang Yuanhui, Wang Liqing, Yin Chenghua, Ji Yishun, Dang Xianmin, Wu Cunrong, Li Zhijian, Ren Zhengdong, Mao Hongxia, Wu Lili, Zhou Hongmei, Song Zewei, Wang Yajun. Determination of Polyphenol Oxidase Activity in Wheat Flour by Grain and Oil Spectrophotometry1 ScopeThis standard specifies the principle, reagents, instruments and equipment, analytical procedures and results of spectrophotometric determination of wheat phenolic oxidase activity. Calculation and precision. This standard applies to the determination of wheat powder polyphenol oxidase activity.2 Normative referencesThe following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods3 Terms and definitionsThe following terms and definitions apply to this document. 3.1 Polyphenol oxidase A class of copper-containing oxidoreductases that catalyze the oxidation of ortho-benzenediol to o-benzidine and also to the monophenol monooxygenase substrate. 3.2 Polyphenol oxidase activity unit Under the measurement conditions specified in this standard, the absorbance value per gram of sample changes by 0.001 to 1 polyphenol oxidase activity unit, U means.4 PrincipleThe polyphenol oxidase in the sample is oxidized with catechol to form hydrazine, and the absorption intensity at a wavelength of 410 nm is measured by a spectrophotometer. Indicates the viability of polyphenol oxidase.5 reagentsUnless otherwise specified, the reagents used in this method are of analytical grade, and the test water should meet the specifications of the secondary water in GB/T 6682. 5.1 catechol solution. Accurately weigh 1.300g of catechol, dissolve it in water, and dilute to a 100mL brown volumetric flask. 5.2 0.2mol/L pH6.0 sodium dihydrogen phosphate-disodium hydrogen phosphate buffer. weigh 31.2g sodium dihydrogen phosphate dihydrate, soluble in appropriate amount of distillation Dilute to 1000 mL in water; weigh 71.6 g of disodium hydrogen phosphate dodecahydrate, dissolve in appropriate amount of distilled water, dilute to 1000 mL; 87.7 mL Sodium dihydrogen phosphate solution and 12.3 mL of disodium hydrogen phosphate solution were mixed to obtain 0.2 mol/L sodium phosphate monobasic phosphate-dihydrogen phosphate Flush. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of LST6124-2017_English be delivered?Answer: Upon your order, we will start to translate LST6124-2017_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. 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