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LST6112-2015 English PDF

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LST6112-2015: Inspection of grain and oils. Detection of zearalenone in grain. Rapid quantitative method of colloidal gold technique
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
LS/T 6112-2015139 Add to Cart 3 days Inspection of grain and oils. Detection of zearalenone in grain. Rapid quantitative method of colloidal gold technique Valid

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Basic data

Standard ID: LS/T 6112-2015 (LS/T6112-2015)
Description (Translated English): Inspection of grain and oils. Detection of zearalenone in grain. Rapid quantitative method of colloidal gold technique
Sector / Industry: Food & Beverage Industry Standard (Recommended)
Classification of Chinese Standard: B20
Classification of International Standard: 67.06
Word Count Estimation: 6,690
Date of Issue: 2015-07-10
Date of Implementation: 2015-07-10
Quoted Standard: GB 5491; GB/T 6682; GB/T 27404-2008
Regulation (derived from): State-Food-Communication [2015] No.3
Issuing agency(ies): National Grain Administration
Summary: This standard specifies the zearalenone colloidal gold rapid quantitative detection method principle, reagents, materials, equipment, sample preparation, sample measurement and results are expressed. This standard applies to the quantitative analysis of wheat, corn and rice and other grains and products of zearalenone. The method detection limit of 5 ��g/kg.

LST6112-2015: Inspection of grain and oils. Detection of zearalenone in grain. Rapid quantitative method of colloidal gold technique


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Inspection of grain and oils.Detection of zearalenone in grain.Rapid quantitative method of colloidal gold technique ICS 67.060 B20 Record number. 50651-2015 People's Republic of China food industry standard Determination of Zearalenone in Grains by Grain and Oil Inspection Colloidal gold rapid quantification Released on.2015-07-10 2015-07-10 implementation National Food Bureau released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009 and GB/T.20001.4-2001. This standard was proposed by the National Grain Administration. This standard is under the jurisdiction of the National Grain and Oil Standardization Technical Committee (SAC/TC270). This standard is drafted by. Shanxi Food Quality Monitoring Center. Participated in the drafting of this standard. Beijing Boou Shide Biotechnology Co., Ltd., Beijing Cereals, Oils and Foodstuffs Inspection Institute, Liaoning Province Grain and Oil Inspection The Institute of Monitoring, the Northeast Regional Laboratory of COFCO, the Xinjiang Grain and Oil Product Quality Supervision and Inspection Station, and the Shandong Grain and Oil Testing Center. The main drafters of this standard. Wang Heng, Song Zewei, Li Hua, Guo Jian, Cui Guohua, Li Huijuan, Ma Hong, Cui Guoyou, Wang Pei. Determination of Zearalenone in Grains by Grain and Oil Inspection Colloidal gold rapid quantification

1 Scope

This standard specifies the principles, reagents, materials, equipment, sample preparation, and methods for rapid quantitative detection of colloidal gold in zearalenone. Sample determination and results presentation. This standard applies to the rapid quantitative detection of zearalenone in wheat, corn and rice and other products. The detection limit of the method was 5 μg/kg.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB 5491 Inspection of food and oil materials, sample method GB/T 6682 Analytical laboratory water specifications and test methods GB/T 27404-2008 Laboratory quality control specification Food physical and chemical testing

3 Principle

The zearalenone in the sample extract reacts with the colloidal gold particles in the test strip, and the color depth is related to the zearalenol in the sample. Ketone content is related. Use a reader to determine the color depth of the test line and the quality control line on the test strip, according to the color depth and the built-in curve of the reader. The zearalenone content in the sample was calculated.

4 reagents

Unless otherwise stated, the reagents used are of analytical grade, and laboratory water should meet the requirements of Class III water in GB/T 6682. 4.1 Dilution buffer. supplied by the zearalenone colloidal gold test strip or prepared according to the product instruction manual. 4.2 Sample extract. 70% (volume fraction) methanol solution.

5 instruments and materials

Laboratory routine instruments, equipment and materials. 5.1 Balance. The division value is 0.01g. 5.2 Crusher. The sample can be pulverized and passed through a 20 mesh sieve. 5.3 Centrifuge. The speed is not less than 4000r/min. 5.4 vortex oscillator. 5.5 Incubator. It can be incubated at 45 °C (temperature control accuracy ± 1 °C) with time adjustment. 5.6 Reader. The measurement result of the colloidal gold quantitative test strip can be measured and displayed.
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