JJF 2023-2023 English PDF

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JJF 2023-2023: Calibration Specification for Rapid Aflatoxin Analyzers
Status: Valid

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JJF 2023-2023	399	Add to Cart	4 days	Calibration Specification for Rapid Aflatoxin Analyzers	Valid

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Basic data

Standard ID: JJF 2023-2023 (JJF2023-2023)
Description (Translated English): Calibration Specification for Rapid Aflatoxin Analyzers
Sector / Industry: Metrology & Measurement Industry Standard
Word Count Estimation: 18,154
Date of Issue: 2023-03-15
Date of Implementation: 2023-09-15
Issuing agency(ies): State Administration for Market Regulation

JJF 2023-2023: Calibration Specification for Rapid Aflatoxin Analyzers

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
National Metrology Technical Specifications of the People's Republic of China JJF 2023-2023 Calibration specification for aflatoxin rapid detector Released on 2023-03-15 2023-09-15 Implementation Released by the State Administration for Market Regulation Aflatoxin rapid test Instrument Calibration Specifications Responsible Unit: National Technical Committee of Physical and Chemical Metrology Main drafting unit: China Institute of Metrology Participating in the drafting unit: Beijing Institute of Metrology and Testing Sciences State Grain and Material Reserve Bureau Scientific Research Institute This specification entrusts the National Technical Committee of Physical and Chemical Metrology to be responsible for interpretation The main drafters of this specification: Ma Kang (National Institute of Metrology) Zhao Haibo (Beijing Institute of Metrology and Testing Sciences) Participating drafters: Zhang Yiwen (Beijing Institute of Metrology and Testing Sciences) Wang Songxue (Scientific Research Institute of the State Administration of Grain and Material Reserves)

Table of contents

Introduction (Ⅱ) 1 Scope(1) 2 References(1) 3 terms(1) 4 Overview(1) 5 Metrological characteristics(2) 5:1 Concentration indication error (2) 5:2 Measurement repeatability(2) 5:3 Detection limit(2) 6 Calibration conditions(2) 6:1 Environmental conditions (2) 6:2 Measuring standards and other equipment (2) 7 Calibration Items and Calibration Methods(2) 7:1 Preparations before calibration(2) 7:2 Concentration indication error (3) 7:3 Measurement repeatability(3) 7:4 Detection limit(3) 8 Expression of Calibration Results(3) 9 Recalibration interval(4) Appendix A Preparation and Application of Series Standard Solutions (5) Appendix B Calibration Original Record Reference Format (6) Appendix C Calibration Certificate Inner Page Reference Format (8) Appendix D Example of Evaluation of Measurement Uncertainty of Instrument Indication Error (9)

Introduction

This standard is based on JJF 1071-2010 "National Measurement and Calibration Standard Compilation Rules", JJF 1001-2011 "General It is prepared according to the relevant provisions of "Measurement Terms and Definitions" and JJF 1059:1-2012 "Assessment and Expression of Measurement Uncertainty": This specification mainly refers to GB 2761-2017 "National Food Safety Standard Limits of Mycotoxins in Food" Limit requirements for aflatoxins; LS/T 6402-2017 "Applicability verification of grain and oil inspection equipment and methods standards and General Principles of Outcome Evaluation: This specification is published for the first time: Calibration specification for aflatoxin rapid detector

1 Scope

This standard applies to the quantitative detection of aflatoxins based on the principle of enzyme-linked immunosorbent immunosorbent or immunochromatography (colloidal gold/fluorescence) Calibration of rapid detectors:

2 References

This specification references the following documents: GB 2761-2017 National Food Safety Standard Limits of Mycotoxins in Food LS/T 6402-2017 Standard Applicability Verification of Grain and Oil Inspection Equipment and Methods and General Principles of Result Evaluation For dated references, only the dated version applies to this specification; for undated references document, its latest version (including all amendments) applies to this specification:

3 terms

The following terms and definitions apply to this document: Using the principle of indirect competitive enzyme labeling, the soluble antigen or antibody is bound to the solid phase carrier such as polystyrene, and the advantage is Qualitative and quantitative detection method of immune response by antigen-antibody specific binding: Using the principle of competitive inhibition immunochromatography, the samples were mixed with colloidal gold-labeled specific monoclonal antibodies during chromatography: Combined with the body, the qualitative and quantitative detection methods of immunochromatography are carried out:

4 Overview

The aflatoxin rapid detector (hereinafter referred to as the detector) is mainly used for the determination of food, feed, traditional Chinese medicine, dairy Aflatoxin content in products, etc: Its detection principle is divided into two types: one is the ELISA method, the aflatoxin in the sample Aflatoxin competes with the Aflatoxin antigen on the ELISA plate for the antibody of aflatoxin, and after color development with the substrate, the corresponding microwell of the sample The absorbance decreases, and the absorbance value is negatively correlated with the aflatoxin content in the sample; the second is immunochromatography (colloidal gold/ Fluorescence) method, the aflatoxin in the sample is combined with the colloidal gold/fluorescence-labeled specific antibody during the chromatography, Thereby inhibiting the combination of the antibody and the aflatoxin conjugate on the nitrocellulose membrane detection line, so that the detection line develops color or The fluorescence intensity decreases, and the color development or fluorescence intensity is related to the aflatoxin content in the sample: The detector consists of an optical system, an image sensor or a photoelectric sensor, a reagent package (enzyme-linked immunoassay kit or immune layer) Analysis test strip/card), signal processing system and data display system, its structure is shown in Figure 1: ......

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