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JJF 1821-2020 English PDF

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JJF 1821-2020: Calibration Specification for Temperature Calibration Devices for Polymerase Chain Reaction Analyzers
Status: Valid
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JJF 1821-2020489 Add to Cart 4 days Calibration Specification for Temperature Calibration Devices for Polymerase Chain Reaction Analyzers Valid

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Basic data

Standard ID: JJF 1821-2020 (JJF1821-2020)
Description (Translated English): Calibration Specification for Temperature Calibration Devices for Polymerase Chain Reaction Analyzers
Sector / Industry: Metrology & Measurement Industry Standard
Classification of Chinese Standard: A54
Classification of International Standard: 17.200
Word Count Estimation: 22,214
Date of Issue: 2020
Date of Implementation: 2020-04-17
Issuing agency(ies): State Administration for Market Regulation

JJF 1821-2020: Calibration Specification for Temperature Calibration Devices for Polymerase Chain Reaction Analyzers


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Calibration specification for temperature calibration device of polymerase chain reaction analyzer) National Metrology Technical Specifications of the People's Republic of China Calibration specification for temperature calibration device of polymerase chain reaction analyzer 2020-01-17 released 2020-04-17 implementation Issued by the State Administration for Market Regulation Polymerase Chain Reaction Analyzer Calibration specification for temperature calibration device Focal point. National Technical Committee for Temperature Measurement Main drafting unit. Shanghai Institute of Metrology and Testing Technology China Institute of Metrology Participating in the drafting. Liaoning Institute of Metrology Jiangsu Institute of Metrology Guangdong Institute of Metrology Shanghai Hongshi Medical Technology Co., Ltd. This specification entrusts the National Temperature Measurement Technical Committee to be responsible for the interpretation The main drafters of this specification. Zhang Liping (Shanghai Institute of Metrology and Testing Technology) Jin Zhijun (China Institute of Metrology) Gao Yunhua (China Institute of Metrology) Participating drafters. Dong Liang (Liaoning Institute of Metrology) Wang Zheng (Jiangsu Institute of Metrology) Liang Xianyou (Guangdong Institute of Metrology) Qin Rong (Shanghai Hongshi Medical Scientific Instrument Co., Ltd.)

table of Contents

Introduction (Ⅱ) 1 scope (1) 2 Reference documents (1) 3 Term (1) 3.1 Polymerase reaction analyzer (1) 4 Overview (1) 5 Measurement characteristics (2) 5.1 Temperature indication (2) 6 Calibration conditions (2) 6.1 Environmental conditions (2) 6.2 Measurement standards and other equipment (2) 7 Calibration items and calibration methods (2) 7.1 Calibration items (2) 7.2 Calibration method (2) 8 Processing of calibration data (5) 9 Expression of calibration results (5) 10 Recheck interval (5) Appendix A Original Record Reference Format (6) Appendix B Reference Format of the Internal Page of the Calibration Certificate (8) Appendix C Description of isothermal block structure (9) Appendix D Test methods for isothermal block temperature uniformity and fluctuation (11) Appendix E Example of Uncertainty Evaluation of Temperature Indication Calibration (13)

Introduction

This specification is based on JJF 1071-2010 "Rules for the Compilation of National Metrology and Calibration Specifications" and JJF 1059.1-2012 Compilation of "Evaluation and Expression of Measurement Uncertainty". This specification is released for the first time. Calibration specification for temperature calibration device of polymerase chain reaction analyzer

1 Scope

This specification is applicable to the calibration of polymerase chain reaction analyzer temperature calibration device whose measuring range is (0~120)℃.

2 Reference documents

This specification refers to the following documents. JJG160-2007 Standard platinum resistance thermometer JJF 1030-2010 Specification for Technical Performance Test of Constant Temperature Bath JJF 1527-2015 Polymerase Chain Reaction Analyzer Calibration Specification YY/T 1173-2010 polymerase chain reaction analyzer For dated reference documents, only the dated version applies to this specification; for undated references The latest version (including all amendments) is applicable to this specification.

3 Term

3.1 Polymerase chain reaction analyzer Based on the principle of polymerase chain reaction (PCR) technology, it simulates the replication process of DNA or RNA. In the presence of primers, polymerase, etc., a specific amplification of a known sequence, detection and analysis of equipment. Also known as gene cycler.

4 overview

The polymerase chain reaction analyzer temperature calibration device consists of a single-channel or multi-channel temperature measurement module, a data acquisition module, and a The structure of the temperature measurement module can simulate the polymerase chain reaction analyzer (with Hereinafter referred to as PCR instrument) The shape and structure of the sample reaction tube is mainly used to calibrate the temperature characteristics of the PCR instrument. Its composition block diagram is shown in Figure 1. (a) Temperature measurement module (b) Data acquisition module (c) Display
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