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Basic dataStandard ID: HJ 710.12-2016 (HJ710.12-2016)Description (Translated English): Technical guidelines for biodiversity monitoring. aquatic vascular plants Sector / Industry: Environmental Protection Industry Standard Classification of Chinese Standard: Z06 Word Count Estimation: 22,264 Date of Issue: 2016-05-04 Date of Implementation: 2016-08-01 Quoted Standard: GB/T 7714; GB/T 8170; HJ 623 Regulation (derived from): Ministry of Environmental Protection Announcement No. 35 of 2016 Issuing agency(ies): Ministry of Ecology and Environment HJ 710.12-2016: Technical guidelines for biodiversity monitoring. aquatic vascular plants---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Technical guidelines for biodiversity monitoring.aquatic vascular plants National Environmental Protection Standard of the People 's Republic of China Technical Guidelines for Biodiversity Observations Aquatic Vascular Plants 2016-5-4 released 2016-8-1 implementation Ministry of Environmental Protection released Directory Foreword1 Scope of application2 normative reference documents3 Terms and definitions 14 Principles of observation5 Observation method6 Observations of content and indicators7 Observation time and frequency8 Data processing and analysis9 Quality control and safety management10 Observations Report 8 Appendix A (informative) List of habitat elements for observing plots Appendix B (informative appendix) List of field observations of water plants Appendix C (Informative Appendix) Floating Plant Community Field Observations Table 11 Appendix D (informative) Record of submerged plant communities in field observations Appendix E (informative) Aquatic Vascular Plants Document Specimen Record Label Appendix F (informative) Classification of human disturbance activities Appendix G (informative) Data processing and analysis methods Appendix H (informative) BRAUN-BLANQUET Median value conversion for multi-coverage levels .18 Appendix I (informative) Hydrological Vascular Plant Observing Report Preparation FormatForewordIn order to implement the Environmental Protection Law of the People's Republic of China, the Regulations of the People's Republic of China on the Protection of Wild Plants and "China's Biodiversity Conservation Strategy and Action Plan" (2011 ~ 2030), regulate China's biodiversity observation work, Develop this standard. This standard specifies the main contents, technical requirements and methods of aquatic vascular plant diversity observation. Appendix A, Appendix B, Appendix C, Appendix D, Appendix E, Appendix F, Appendix G, Appendix H, Appendix I Information appendix. This standard is the first release. This standard is in line with the following criteria and is a series of technical guidelines for biodiversity observation. Guidelines for the Observations of Biodiversity - Terrestrial Vascular Plants (HJ 710.1); Guidelines for Biodiversity Observations Lichen and Moss (HJ 710.2); Guidelines for Biodiversity Observations Terrestrial Mammals (HJ 710.3); Biodiversity Observations Technical Guidelines Birds (HJ 710.4); Guidelines for Biodiversity Observations Reptiles (HJ 710.5); Technical Guidelines for Biodiversity Observations Amphibians (HJ 710.6); Guidelines for the observation of biological diversity Inland water fish (HJ 710.7); Guidelines for Biodiversity Observations Freshwater Benthic Large Invertebrates (HJ 710.8); Guidelines for Biodiversity Observations Butterfly (HJ 710.9); Technical Guidelines for Biodiversity Observations Large and Medium Soil Animals (HJ 710.10); Technical Guidelines for Biodiversity Observation Large Fungi (HJ 710.11); Guidelines for Biodiversity Observations Bee (HJ 710. □□). This standard is organized by the Ministry of Environmental Protection Science and Technology Standards Division. The main drafting of this standard. Nanjing Normal University, Ministry of Environmental Protection Nanjing Environmental Science Research Institute. The environmental protection department of this standard approved on May 4,.2016. This standard has been implemented since August 1,.2016. This standard is explained by the Ministry of Environmental Protection. Technical Guidelines for Biodiversity Observations Aquatic Vascular Plants1 Scope of applicationThis standard specifies the main contents, technical requirements and methods of aquatic vascular plant diversity observation. This standard applies to the diversity of aquatic vascular plants within the People's Republic of China.2 normative reference documentsThe contents of this standard refer to the following documents or their terms. For those who do not have a date reference, the latest version applies In this standard. GB/T 7714 after the reference of the literature Representation and Judgment of Numeric Ratification Rule and Limit Value of GB/T 8170 HJ 623 Regional Biodiversity Assessment Criteria3 terms and definitionsThe following terms and definitions apply to this standard. 3.1 Vascular plant Refers to plants with vascular tissue, including ferns, gymnosperms and angiosperms. 3.2 Aquatic vascular plant Refers to a vascular plant that lives in water or floats on the surface for at least several months of the year. According to the different life type, usually points For the water plants, water plants and submerged plants. 3.3 Water plant emergent plant Refers to the root born in the bottom, stem upright, photosynthetic tissue aerial plants. 3.4 Floating plant A plant that floats, leaves, or floats. 3.5 Submerged plant Refers to plants that are planted in the subsurface during the majority of their life cycle. 3.6 Dominant species Refers to the community structure and community environment has obvious control of the formation of species, usually the number of individuals, projection Cover large, high biomass, strong vitality of plant species. 3.7 Invasive alien aquatic plant Refers to the formation of self-sustaining capacity in the local natural or semi-natural waters ecosystem, which may or may have been Such as exotic plants that cause significant adverse effects. 3.8 Life form Refers to the type of plant that is reflected in appearance for long periods of adaptation under specific environmental conditions. 3.9 Point-intercept method A method for determining the coverage of species in herbaceous plants in the wild. Usually measured using a point frequency frame (figure 1). Figure 1 Point interception method used in the point frequency frame 3.10 Multi-coverage level method cover -abundance scale method Braun-Blanquet uses the visual method to estimate the individual species of a plant species in the Phillips school of plant community studies The extent of coverage in the community, and the extent of coverage of the plant's foliage in the area per unit area. The method usually places the plant more And coverage is divided into five levels, two auxiliary levels, mainly with the figures are as follows. 5 = regardless of the number of individuals, coverage ≥ 75%; 4 = regardless of the number of individuals, 75% > coverage ≥ 50%; 3 = no matter how many individuals, 50% > coverage ≥ 25%; 2 = regardless of the number of individuals, 25%> coverage ≥ 5%, or cover the degree of < 5%, but the number of individuals; 1 = the number of individuals more, coverage < 5%, or although the coverage ≥ 5%, but the number of individuals is rare; = Individual number is scarce, coverage ≤ 1%; R = coverage is very small, the number of individuals is very small (usually only 1 to 3). 3.11 Frequency Refers to the percentage of an aquatic plant that appears in all survey samples. In some literature also known as the absolute frequency (Absolute frequency). 3.12 Absolutely vibrant Refers to the percentage of the sum of the number of individuals appearing on a sample in a sample line and the total number of samples surveyed. 3.13 Cover index Refers to the sum of the frequency and absolute vigor of a species. 3.14 Biomass Refers to the total mass of all plant bodies per unit area.4 Observational principles4.1 Scientific principles The observation sample and the observation object should be representative, which can fully reflect the overall condition of aquatic vascular plants in the observation area. A unified and standardized observation method should be used to observe the dynamic changes of aquatic vascular diversity. 4.2 Principles of operability The observing plan should take into account the human, financial and logistical support conditions, make full use of available information and results, Based on existing observation equipment and personnel conditions, high efficiency, low cost and operable observation methods should be used. 4.3 Principles of Continuity Observations should meet the needs of biodiversity conservation and management and can play a role in biodiversity conservation and management. Guidance and early warning role. Observations, samples and samples once determined, not free to change. 4.4 Principles of protection Adhere to the protection of the first, unless the species identification and biomass measurement need to collect a small number of individuals, as much as possible to reduce the sample And should not affect or alter aquatic plant individuals, community structures and habitats. 4.5 Safety principles Aquatic vascular plant observations may face tides, wind and other potential risks. Observers should receive relevant professional training, Do security measures.5 Observation method5.1 Preparation for observation 5.1.1 Observation objectives and observation areas The objective of the observation is to grasp the species, population number, distribution pattern and distribution type of aquatic plants in the area to be observed And changes in dynamics, or analysis of species composition, geographical distribution and population dynamics of invasive alien invasive plants Plants, which do not need to be observed); or analyze the effects of various threat factors on aquatic plant diversity; Aquatic plant protection measures and the effectiveness of policies, and put forward targeted management measures. According to the observation target to determine the observation area. 5.1.2 Data collection and observation plan According to the observation objectives and requirements, as far as possible to collect the observation area topographic map, vegetation distribution map, climate, hydrology, soil And other basic data, and develop observation plans. If necessary, can be carried out in advance a field trip. The observation plan should include. sample lines Settings, sample (or sample) settings, field observations, observations and indicators, observation time and frequency, data points Analysis and reporting, quality control and safety management. 5.1.3 Observation instruments and tools 5.1.3.1 Plant sample collection and recording tools Including trumpet, tweezers, iron clip (or iron rake), Peterson mud collection, collection bag, sample bag (or plastic self-styled Bag), plastic bottle, magnifying glass, branch scissors, sampling box (side length 1 m or 0.5 m), gauze, with water absorption Grass paper, corrugated board, specimen clip, long tape, steel tape, hand hammer, nail, standard pile (1.5 m long, 50 mm thick PVC or other material pipe), stakes, plastic rope, length 100 cm × height 100 cm point frequency frame (on the set 10 Equidistant pinhole, that is, between the two holes separated by 10 cm; practical application, the size of the frame and the number of pinholes and metal needle between Separated according to the size and spacing of plants to adjust), metal needle, with a macro lens digital camera, record table, Label paper, marker, professional tool book and so on. 5.1.3.2 Aquatic plant habitat observation instruments and tools Including binocular telescopes, Global Positioning System (GPS) locator, compass, pH meter, thermometer, transparent disc, Aquatic folder, oven, electronic balance, echo measuring device, SCUBA (underwater respirator) or snorkel (ventilation tube), Measuring rod, water mound, dissolved oxygen instrument, waterproof overalls, high tube rubber boots, rubber gloves and so on. Rivers and large lakes to be rented With the ship. 5.1.4 observation training For the observation of observation methods and practices such as training, organize a good observation team. 5.2 Sampling methods 5.2.1 According to the different living type, the aquatic plants are divided into water plants, water plants and submerged plants. According to the observation purpose , The characteristics of the water environment and the distribution of different types of aquatic plants, the use of systematic sampling and the combination of typical sampling Method, layout, sample or sample. 5.2.2 Marking samples, samples and samples using GPS or other means, mark the location on the topographic map and record The habitat of the plot (see Appendix A for the record form). 5.2.3 According to the different types of water vegetation, water conditions, the degree of interference set the sample line. Dominant species of the same or similar The type of vegetation can be set along the edge of the water 3 to 5 lines; line length as the water area, habitat heterogeneity Degree, generally 800 ~ 1000 m. The arrangement, number and length of the sample lines shall be adjusted in accordance with the actual size of the water body. For the community (or habitat) type of more complex water, may be appropriate to increase the number of lines, usually 5 to 7, while Shorten the length of the sample line. The spacing between the lines is generally not less than 250 m, according to the actual situation for a certain adjustment. In every On the line, set one sample every 50 m. The area of the sample is 2 m × 2 m. Divide the sample from the center of the sample For the four 1 m × 1 m sample (Fig. 2), the sample frequency is used for each sample to be investigated. The frequency frame has a width of 100 cm and a metal needle. Figure 2 Schematic diagram of water plant sample and sample layout 5.2.4 According to the different types of floating vegetation, water environment characteristics and the degree of interference, the lake, rivers, reservoirs, etc. Large-scale water is divided into the entrance area, deep water area (or lake area), export area, sub-coastal zone, coastal zone, or pollution area and Relative to the clean area and other different regions, in these areas were set up a number of representative cross-section. Cross section setting According to the level of detail of the survey, the number of dominant species, the speed of water and water quality of the water may be. Cross section between Interval is generally not less than 250 m, according to the actual situation for a certain adjustment. Set the line in each cross section on each section On a sample line at a certain distance (depending on the actual situation in the field) to set the sample, or from the shore of the water to the center of the water From the layout of the square, until a certain depth of the water so far (Figure 3a). For slow or even static water, or shallow water depth Of the pond or river branch, etc., can be arranged in each sample line sample side (Figure 3b). The area of the sample is 1 m × 1 m. In each of them The sample is based on the point frequency frame survey in the sample interception method. The frequency frame has a width of 100 cm and a 10 metal needle (figure 1). 5.2.5 According to the different types of submerged vegetation, the characteristics of water environment and the degree of human disturbance, the lake, river, water Reservoirs and other large water bodies are divided into the entrance area, deep water area (or lake area), export area, coastal zone, coastal zone, or pollution Areas and relative clean areas, and a number of representative cross sections are provided in these areas. Cross section According to the level of detail of the survey, the number of dominant species of aquatic plants, the speed of water and the transparency of the water may be. The interval between the cross-section is generally not less than 250 m, according to the actual situation for a certain adjustment. Set on each broken face Sample lines, in each sample line at a certain distance from the set of samples, or from the shore of the water body to the center of the water equidistant Side until a certain depth of water (Figure 4). For slow or even static water, or shallow depth of the pond or river branch Etc., can be arranged in each sample line were square. Each sample (usually area of 2 m × 2 m, also according to submerged vegetation Of the actual situation appropriate adjustment) is divided into four small sample, each sample area of 1 m × 1 m. In every little The Braun-Blanquet multi-coverage method was used to investigate. Figure 3 Schematic diagram of the layout of water samples and sample layout Note. The dotted line is the sampling line, the dots are the sampling points; the solid dots indicate the shallow water area where the floating water plants are more concentrated, The hollow dots indicate the deepwater areas where the floating water plants are less distributed. (A) a large water body, such as a lake or a river; (b) a pool Pond or ditch and other small water bodies. Figure 4 submerged plant survey sample line and sample layout diagram Note. The dotted line is the sampling line, the dot is the sample; the solid dot indicates the shallow water area where the submerged plant is more concentrated, The heart dots indicate the deepwater areas where the submerged plants are less distributed. 5.3 Field observation 5.3.1 Data collection 5.3.1.1 For the plant communities, the sides of the frequency frame are fixed vertically at the midpoint of the opposite sides of the sample, And then a metal needle from the middle of the middle of the frame plus the vertical hole into the vertical insert, record the metal needle from the top down touch The number, number, height and phenological period of all aquatic plants (see Appendix B for the record). To minimize errors, metal The tip diameter of the needle is not more than 3 mm. When using the sample interception method, should try to avoid the wind in the weather, the number of samples Head is generally not less than.200, but can be adjusted according to the water area to ensure that sampling is representative. If it is quite Water vegetation cover large or tall plants, you can use the tilt point of the intercept method (inclined point-intercept method) The frequency frame is tilted 32.5 ° to the vertical plane. 5.3.1.2 For the floating plant community, due to the use of 10 metal needles, need to record each needle from the top down The type, number, height and phenological period of all aquatic plants touched (see Appendix C for the record form). To reduce water shaking, Can be 2 people with. That is, one hand-held frame perpendicular to the surface of the water, and the other one were 10 needle in turn from left to right Straight down to insert, record the relevant data. The number of samples is generally not less than.200, but can be appropriate according to the water area Adjustment. 5.3.1.3 For submerged plant communities, record the types, coverage and multi-coverage grades of submerged plants in each sample See Appendix D for the table. The plants recorded in the sample should be naturally submerged, rather than submerged with water Things. Except for a few submerged or submerged plants that rely on the branches of the leaves to reproduce or diffuse. In the use of visual assessment In the sample, the coverage of each plant should be consistent as much as possible. If conditions permit, you can consider using Hydroacoustic survey, and the use of SCUBA (underwater breathing apparatus) or snorkel (ventilation tube) And other equipment diving, record the distribution of submerged plants and population density and other aspects of the content. 5.3.1.4 Biomass determination of......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of HJ 710.12-2016_English be delivered?Answer: Upon your order, we will start to translate HJ 710.12-2016_English as soon as possible, and keep you informed of the progress. The lead time is typically 2 ~ 4 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of HJ 710.12-2016_English with my colleagues?Answer: Yes. 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