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GB/T 44214-2024 English PDF

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US$279.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 44214-2024: Test method for non-protein content in silk
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 44214-2024	279	Add to Cart	3 days	Test method for non-protein content in silk	Valid

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Basic data

Standard ID: GB/T 44214-2024 (GB/T44214-2024)
Description (Translated English): Test method for non-protein content in silk
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: W40
Classification of International Standard: 59.080.01
Word Count Estimation: 14,123
Date of Issue: 2024-07-24
Date of Implementation: 2025-02-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 44214-2024: Test method for non-protein content in silk

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Test method for non-protein content in silk Test method for content of non-silk protein substances in silk ICS 59.080.01 CCS W 40 National Standard of the People's Republic of China Released on 2024-07-24 2025-02-01 Implementation State Administration for Market Regulation The National Standardization Administration issued

Preface ... 1 Scope ... 2 Normative referenced documents ... 3 Terms and Definitions ... 4 Principle ... 5 Reagents and Materials ... 6 Instruments and Equipment ... 7 Test procedures ... 7.1 Preparation of standard solution ... Thai Silk Group Co., Ltd., Zhejiang Institute of Inspection and Quarantine, Hangzhou Quality and Technical Supervision Institute, Shanghai Quality Supervision Institute Inspection Technology Research Institute, Zhejiang Eco-textile Prohibited Dyes and Chemicals Testing Center Co., Ltd., Jiangsu Engineering Vocational and Technical College, SMIC Biotechnology (Hangzhou) Co., Ltd., Suzhou Intellectual Property Protection Center, Shaoxing Keqiao District China Textile City Inspection, Testing and Standardization Research Institute Research Institute. The main drafters of this document are. Wu Guojian, Wu Dongping, Chen Siyu, Sun Chenxiao, Qi Ning, Liu Yu, Jiang Zhaohui, Zhao Hailang, Chen Fengming, Liu Wei, Lei Bin, Ni Xuelin, Jin Feng, He Guojun, Wen Chaojun, Zhu Yangrui, Hang Zhiwei, Li Ting, Cao Shimiao, Chen Zhihua, Yin Guibo, Pan Lulu, Jiang Wei, Feng Guihua, Tong Jiajia. Test method for content of non-silk protein substances in silk

1 Scope

This document describes a test method for the determination of the content of non-silk protein substances in silk. This document is applicable to the calculation of silk by determining the content of four main amino acids (serine, glycine, alanine and tyrosine) in silk. The content of non-silk protein substances in it.

2 Normative references

The contents of the following documents constitute the essential clauses of this document through normative references in this document. For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies. in this document. GB/T 6682 Specifications and test methods for water used in analytical laboratories GB/T 8170 Rules for rounding off values and expression and determination of limit values GB/T 9995 Determination of moisture content and moisture regain of textile materials - Oven drying method

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Non-protein content The total amount of substances that have not been decomposed into amino acids after silk is hydrolyzed, including the amount of free NH4 that exceeds the normal range.

4 Principle

Silk is mainly composed of fibroin and sericin, which can be hydrolyzed into free amino acids in high-temperature acidic solutions. After reacting with phenyl isothiocyanate, The generated amino acid derivatives have strong absorption peaks at ultraviolet wavelengths. After separation by chromatographic column, high performance liquid chromatography was used to analyze the four The main amino acids (serine, glycine, alanine, tyrosine) are determined. Because the amino acid content in silk is relatively stable, The content of amino acids after hydrolysis of silk protein can be calculated to obtain the content of non-silk protein substances in silk. Note. Currently, the non-protein substances in silk mainly include grafted polymers with methacrylamide as the monomer.

5 Reagents and Materials

5.1 Concentrated hydrochloric acid. high purity. 5.2 Acetonitrile. chromatographic grade. 5.3 Phenyl isothiocyanate. purity ≥98%. 5.4 Triethylamine. analytical grade. 5.5 n-Hexane. chromatographic grade. 5.6 Acid hydrolysis solution. 6 mol/L hydrochloric acid (5.1) solution. 5.7 Phenyl isothiocyanate x acetonitrile solution (1 mol/L). Pipette 1.3 mL of phenyl isothiocyanate into a 10 mL volumetric flask and dilute to volume with acetonitrile. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 44214-2024_English be delivered?

Answer: Upon your order, we will start to translate GB/T 44214-2024_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 44214-2024_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 44214-2024_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.