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Chemicals - Protozoan activated sludge inhibition test
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Basic data
| Standard ID | GB/T 42367-2023 (GB/T42367-2023) |
| Description (Translated English) | Chemicals - Protozoan activated sludge inhibition test |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | A80 |
| Classification of International Standard | 13.300, 13.020 |
| Word Count Estimation | 18,111 |
| Date of Issue | 2023-03-17 |
| Date of Implementation | 2023-10-01 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 42367-2023: Chemicals - Protozoan activated sludge inhibition test
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS13:300;13:020:40
CCSA80
National Standards of People's Republic of China
Chemical Protozoa Activated Sludge Inhibition Test
Released on 2023-03-17
2023-10-01 implementation
State Administration for Market Regulation
Released by the National Standardization Management Committee
table of contents
Preface III
Introduction IV
1 Scope 1
2 Normative references 1
3 Terms and Definitions 1
4 Test Principle 2
5 Test substance information 2
6 Reference 2
7 Test preparation 2
8 Test procedure 4
9 Quality Assurance and Quality Control 5
10 Data processing 5
11 report 7
Appendix A (informative) Test water 8
Appendix B (informative) Substrate 10
Appendix C (Informative) Digitonin Solution 11
Appendix D (informative) concentration-effect curve 12
Reference 13
foreword
This document is in accordance with the provisions of GB/T 1:1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules for Standardization Documents"
drafting:
Please note that some contents of this document may refer to patents: The issuing agency of this document assumes no responsibility for identifying patents:
This document is proposed and managed by the National Standardization Technical Committee on Hazardous Chemicals Management (SAC/TC251):
This document was drafted by: Institute of Microbiology, Guangdong Academy of Sciences (Guangdong Microbial Analysis and Testing Center), Ministry of Ecology and Environment, Solid Waste
With Chemical Management Technology Center, China Academy of Inspection and Quarantine, Xinanrun (Beijing) Consulting Co:, Ltd:, Xiamen Green Biotechnology
Co:, Ltd:, Guangdong Environmental Protection Engineering Vocational College, Guangdong Quanqing Testing Co:, Ltd:, Xi'an Kaijinzhe Testing Co:, Ltd:, Jinjiu Technology Co:, Ltd:
Ltd:
The main drafters of this document: Mei Chengfang, Deng Guirong, Yang Shan, Dou Congcong, Luo Fengjuan, Chen Yanling, Huang Youda, Liu Chunxin, Zeng Guoqu, Liu Yanzhen,
Long Weinian, Zhou Lili, Xu Zhirui, Peng Yijun, Wu Caichun, Mao Xiaoyao:
Introduction
The principle of biological treatment in a domestic sewage treatment plant (STP) is to convert the organic matter contained in the influent sewage into microbial biomass:
The amount of matter, and then the microorganisms are separated from the liquid to obtain purified sewage, with the purpose of minimizing the organic load in the sewage and reducing the
The content of nutrients such as ammonia nitrogen, nitrate and phosphate, while minimizing the amount of biological sludge: biophagocytosis of activated sludge
A process played a role in promoting: In conventional wastewater treatment plants, the above-mentioned phagocytosis is usually dominated by ciliates, which feed on bacteria,
Purify the sewage, further improve the transparency of the sewage, and reduce the organic load in the effluent:
The purpose of this method is to evaluate the impact of chemical substances on consumers in sewage treatment plants, which mainly consist of ciliated protozoa:
composition:
This test is based on an international comparative study, and the relevant test methods have been validated in 5 laboratories: The above research used
Five single-component organic compounds assumed to have non-polar or polar narcotic mode of action (MOA) were used as test substances, namely: 1-octylamine
(CAS: 111-86-4), 3,5-Dichlorophenol (CAS: 591-35-5), Dimethyl Sulfoxide (CAS: 67-68-5), Diphenyl Ether (CAS: 101- 84-8)
and hexachlorophene (CAS: 70-30-4), the partition coefficients of these substances ranged from -1:36 to 6:91:
If this method is used to test the mixture and the data obtained are used for regulation, it is necessary to consider whether the test results are sufficient and valid:
If there are regulations that clearly stipulate that the mixture can be tested, this document can be directly used:
Chemical Protozoa Activated Sludge Inhibition Test
1 Scope
This document describes the test principle, test substance information, reference substance, test preparation, test
Procedures, Quality Assurance and Quality Control, Data Processing, Reporting:
This document applies to protozoan activated sludge inhibition tests for chemicals:
2 Normative references
The contents of the following documents constitute the essential provisions of this document through normative references in the text: Among them, dated references
For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
this document:
GB/T 21801 Rapid Biodegradability Respirometric Test of Chemicals
GB/T 21802 MITI Test for Improved Rapid Biodegradability of Chemicals (I)
GB/T 21803 Rapid biodegradability of chemicals DOC reduction test
GB/T 21831 Rapid Biodegradability Test of Chemicals by Closed Bottle Method
GB/T 21845 Water Solubility Test of Chemicals
GB/T 21852 Chemical partition coefficient (n-octanol-water) high performance liquid chromatography test
GB/T 21853 Partition coefficient of chemicals (n-octanol-water) shake flask test
GB/T 21856 Carbon dioxide generation test for rapid biodegradability of chemicals
GB/T 21857 OECD Screening Test for Improved Rapid Biodegradability of Chemicals
GB/T 22228 Static method for the determination of the vapor pressure of solid and liquid industrial chemicals in the range of 10-1Pa to 105Pa
GB/T 22229 Determination of vapor pressure level of solid and liquid vapor pressure of industrial chemicals in the range of 10-3Pa to 1Pa
Equity
GB/T 27850 General rules for ready biodegradability of chemicals
EN15934 Sludge, treated biological waste, soil and waste Calculation of dry matter by determination of dry matter content or moisture
3 Terms and Definitions
The following terms and definitions apply to this document:
3:1
In a given test period, compared with the control group, the concentration of the test substance that causes the phagocytic activity of the activated sludge to decrease by x%:
3:2
During a given test period, the highest subject with no statistically significant effect (p ≥ 0:05) on the test group compared with the control group
Test substance concentration:
...