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GB/T 40486-2021 English PDF

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US$199.00 · In stock
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GB/T 40486-2021: Determination of melittin content in bee venom powder - High performance liquid chromatography
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 40486-2021	199	Add to Cart	3 days	Determination of melittin content in bee venom powder - High performance liquid chromatography	Valid

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Basic data

Standard ID: GB/T 40486-2021 (GB/T40486-2021)
Description (Translated English): Determination of melittin content in bee venom powder - High performance liquid chromatography
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B47
Word Count Estimation: 10,185
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 40486-2021: Determination of melittin content in bee venom powder - High performance liquid chromatography

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of melittin content in bee venom powder - High performance liquid chromatography ICS 65.140 CCSB47 National Standards of People's Republic of China Determination of bee venom hemolytic peptide content in bee venom dry powder High performance liquid chromatography Released on 2021-08-20 2022-03-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents" Drafting. Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents. This document was proposed by the All-China Federation of Supply and Marketing Cooperatives. This document is under the jurisdiction of the National Bee Product Standardization Working Group. Drafting organizations of this document. Institute of Bee Research, Chinese Academy of Agricultural Sciences, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences. The main drafters of this document. Li Yi, Zhou Jinhui, Huang Jingping, Wang Peng, Zhang Yongxin. Determination of bee venom hemolytic peptide content in bee venom dry powder High performance liquid chromatography

1 Scope

This document specifies the high performance liquid chromatography analysis method for the determination of bee venom hemolytic peptide content in bee venom dry powder. This document is applicable to the determination of bee venom hemolytic peptide content in bee venom dry powder.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to This document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Terms and definitions

There are no terms and definitions that need to be defined in this document.

4 Principle

Bee venom hemolytic peptide is the main biologically active substance of bee venom. The bee venom hemolytic peptide in the sample is easily soluble in water, and the maximum ultraviolet absorption wavelength is 220nm. Therefore, the bee venom dry powder sample is dissolved in pure water, and the extract is filtered and measured by a high-performance liquid chromatograph equipped with an ultraviolet detector. Quantification by external standard method.

5 Reagents and materials

The water is the first grade water that meets the requirements of GB/T 6682. 5.1 Reagents 5.1.1 Acetonitrile. chromatographically pure. 5.1.2 Trifluoroacetic acid. chromatographically pure. 5.2 Standard products Bee venom hemolytic peptide (melittin, CAS. 20449-79-0) purity ≥ 97%. 5.3 Preparation of solution 5.3.1 Bee venom hemolytic peptide standard stock solution (10 mg/mL). accurately weigh 100 mg of the standard substance of bee venom hemolytic peptide into a 10 mL volumetric flask In the medium, dissolve and dilute to the mark with water. 5.3.2 Bee Venom Hemolytic Peptide Standard Working Solution. Measure appropriate amount of standard stock solution and dilute with water to a mass concentration of 20 μg/mL, ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 40486-2021_English be delivered?

Answer: Upon your order, we will start to translate GB/T 40486-2021_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 40486-2021_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 40486-2021_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.