GB/T 40472-2021 English PDF

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GB/T 40472-2021: Detection and identification of Cronartiaceae using real-time PCR
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Basic data

Standard ID: GB/T 40472-2021 (GB/T40472-2021)
Description (Translated English): Detection and identification of Cronartiaceae using real-time PCR
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Word Count Estimation: 10,118
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 40472-2021: Detection and identification of Cronartiaceae using real-time PCR

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Cronartiaceae using real-time PCR ICS 65.020.01 CCSB16 National Standards of People's Republic of China Real-time Fluorescent PCR Quarantine and Identification Method of Cylindricae Released on 2021-08-20 2022-03-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents" Drafting. Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents. This document was proposed and managed by the National Plant Quarantine Standardization Technical Committee (SAC/TC271). Drafting organizations of this document. Ningbo Customs of the People's Republic of China, Institute of Microbiology, Chinese Academy of Sciences, Chinese Academy of Inspection and Quarantine, Urumqi Customs of the People's Republic of China. The main drafters of this document. Duan Weijun, Cai Lei, Zhao Peng, Guo Lixin, Liu Fang, Li Xuelian, Zhang Yongjiang, Zhang Huili, Wang Chong, Chen Xianfeng. Real-time Fluorescent PCR Quarantine and Identification Method of Cylindricae

1 Scope

This document describes the real-time fluorescent polymerase chain reaction (hereinafter referred to as real-time fluorescent PCR) quarantine of the plant pathogenic fungus Cylindricaceae Identification method. This document is applicable to the detection and screening of the plants and their products for the bacteria of the cylindrosis.

2 Normative references

There are no normative references in this document.

3 Terms and definitions

There are no terms and definitions that need to be defined in this document.

4 Basic information

Scientific name. Cronartiaceae Chinese scientific name. Cylindricae Taxonomic status. belonging to Fungi, Basidimycota, Pucciniomycetes, Pucciniomycetes Items (Pucciniales). Transmission route. Cylindrossidae is mainly spread by seedlings or diseased bodies. It can also be spread by wind, rain and insects. See Appendix A for the basic information of Pucciniaceae.

5 Principles of Identification

According to the characteristics of the DNA sequence of Pucciniaceae, the real-time fluorescent PCR method is used to apply the designed primers and probes to the Puccinia spp. Branch pathogens are tested and identified.

6 Instruments and reagents

6.1 Instrument and equipment, experimental utensils Balance, mortar, water bath, pure water meter, vortex oscillator, desktop centrifuge, ultraviolet spectrophotometer, high-speed refrigerated centrifuge, refrigerator, laboratory Fluorescence PCR machine, micropipette (0.1μL~2.5μL, 1μL~10μL, 10μL~50μL, 20μL~200μL, 100μL~ 1000μL). 6.2 Reagents Cetyltrimethylammonium bromide (CTAB) extract (2% CTAB, 1.4 mol/L NaCl, 20 mmol/LEDTA, 100mmol/LTris·HClpH8.0), tris (Tris) saturated phenol, chloroform, isoamyl alcohol, isopropanol, anhydrous ethyl ......

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