GB/T 40455-2021 English PDFUS$359.00 · In stock
Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 40455-2021: Detection and identification of Blueberry shock virus Status: Valid
Basic dataStandard ID: GB/T 40455-2021 (GB/T40455-2021)Description (Translated English): Detection and identification of Blueberry shock virus�� Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B16 Word Count Estimation: 18,163 Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration GB/T 40455-2021: Detection and identification of Blueberry shock virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Detection and identification of Blueberry shock virus ICS 65.020.01 CCSB16 National Standards of People's Republic of China Quarantine and identification method of blueberry shock virus Released on 2021-08-20 2022-03-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee ForewordThis document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents" Drafting. Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents. This document was proposed and managed by the National Plant Quarantine Standardization Technical Committee (SAC/TC271). This document was drafted by. Fuzhou Customs of the People's Republic of China, Fruit Research Institute of Fujian Academy of Agricultural Sciences, China Inspection and Quarantine Research Institute Research Institute, Fujian Agriculture and Forestry University, Xiamen Customs of the People’s Republic of China. The main drafters of this document. Shen Jianguo, Xie Lixue, Zhang Yongjiang, Cai Wei, Chen Xihong, Li Tao, Gao Fangluan, Li Min, Liao Furong. Quarantine and identification method of blueberry shock virus1 ScopeThis document specifies the quarantine and identification method for Blueberryshockvirus. This document is applicable to the quarantine and identification of blueberries that may carry blueberry shock virus.2 Normative referencesThe contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to This document. GB/T 6682 Analytical laboratory water specifications and test methods SN/T 2122 Quarantine sampling method for entry and exit plants and plant products3 Terms and definitionsThere are no terms and definitions that need to be defined in this document.4 Classification information of blueberry shock virusChinese name. blueberry shock virus. Scientific name. Blueberryshockvirus, abbreviation. BlShV. Taxonomic status. Bromoviridae, Ilarvirus. For the host range, disease symptoms, distribution area, transmission route, corpuscle morphology, genome and other information of blueberry shock virus, please refer to the attachment Record A.5 Principles of the methodThe serological and molecular biological characteristics of blueberry shock virus are the main basis for formulating this quarantine identification method. According to BlShV and antibodies The specific reaction between the samples is tested by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and immunochromatographic test strips; Perform nested RT-PCR, immune capture nested RT-PCR, and real-time fluorescent RT-PCR detection according to the characteristics of the BlShV genome; through these methods Effective combination of the method to determine whether the sample carries BlShV. 6 Instruments, utensils and reagents 6.1 Apparatus High-speed refrigerated centrifuge, autoclave, electronic balance (sensitivity 0.001g), microplate reader, PCR instrument, real-time fluorescent PCR instrument, horizontal electric Swimming system, conventional refrigerator, ultra-low temperature refrigerator, gel imaging analysis system, pH meter, constant temperature water bath, etc. 6.2 Utensils Mortar, enzyme-linked plate, centrifuge tube, PCR reaction tube, adjustable pipette of various volumes (2.5μL, 10μL, 20μL, 100μL,.200μL, ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 40455-2021_English be delivered?Answer: Upon your order, we will start to translate GB/T 40455-2021_English as soon as possible, and keep you informed of the progress. 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