GB/T 40140-2021 English PDF

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GB/T 40140-2021: Detection and identification of grape spike-stalk brown spot
Status: Valid

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Basic data

Standard ID: GB/T 40140-2021 (GB/T40140-2021)
Description (Translated English): Detection and identification of grape spike-stalk brown spot
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Word Count Estimation: 14,142
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 40140-2021: Detection and identification of grape spike-stalk brown spot

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of grape spike-stalk brown spot ICS 65.020.01 CCSB16 National Standards of People's Republic of China Quarantine and identification method of grape rot Released on 2021-05-21 2021-12-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents" Drafting. Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents. This document was proposed and managed by the National Plant Quarantine Standardization Technical Committee (SAC/TC271). Drafting organizations of this document. Chinese Academy of Inspection and Quarantine, Ningbo Customs of the People's Republic of China. The main drafters of this document. Wu Pinshan, Duan Weijun, Lei Rong, Xu Han. Quarantine and identification method of grape rot

1 Scope

This document specifies the methods for the isolation, culture, morphological identification, and molecular biological identification of the pathogen of grape rot. This document is applicable to the quarantine and identification of A. vulgaris on grapes.

2 Normative references

There are no normative references in this document.

3 Terms and definitions

There are no terms and definitions that need to be defined in this document.

4 Classification information of Rhizoctonia solani

Chinese name. Rhizoctonia solani Taxonomic status. Fungi, Ascomycota, Dothideomycetes, Pleospo- rales, Pleosporaceae. See Appendix A for other information on the pathogen of grape rot.

5 Principles of the method

According to the disease symptoms, the morphological characteristics and molecular biological characteristics of the isolate, and the specificity of the fungal DNA conserved region PCR or real-time fluorescent PCR detection, through the change of electrophoretic band size or amplification curve, comprehensive quarantine and identification of pathogens. 6 Reagents, materials and culture media 6.1 Reagents and materials Unless otherwise specified, all test reagents are analytical reagents or biochemical reagents. Reagents include. sodium hypochlorite solution (active chlorine >10%), agar powder, glucose, agarose, sterile double distilled water, CTAB extraction buffer Liquid, Tris saturated phenol, chloroform, isoamyl alcohol, isopropanol, absolute ethanol, RNaseA, liquid nitrogen, phenol, chloroform, proteinase K ......

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