GB/T 40049-2021 English PDF

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GB/T 40049-2021: PCR detection method of chicken salmonella enteritis
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 40049-2021	299	Add to Cart	3 days	PCR detection method of chicken salmonella enteritis	Valid

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Basic data

Standard ID: GB/T 40049-2021 (GB/T40049-2021)
Description (Translated English): PCR detection method of chicken salmonella enteritis
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Word Count Estimation: 16,164
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 40049-2021: PCR detection method of chicken salmonella enteritis

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
PCR detection method of chicken salmonella enteritis ICS 11.220 B41 National Standards of People's Republic of China PCR Detection Method of Chicken Salmonella Enteritidis Released on 2021-04-30 2021-11-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China. This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SC/TC181). Drafting organizations of this standard. Shandong Agricultural University, Qingdao Agricultural University, China Center for Animal Health and Epidemiology, Shandong Academy of Agricultural Sciences Poultry Research Institute, Qinhuangdao Customs, Shandong Parker Testing Technology Co., Ltd. The main drafters of this standard. Chang Weishan, Sun Shuhong, Wang Shubai, Zhai Haihua, Cui Yanshun, Chai Tongjie, Ju Zijing, Zhao Xiaonan, Wang Tao, Huang Bing, Song Minxun, Ma Xiuli, Gao Yuehua, Zheng Deyun, Guo Shuyuan, Ma Hongchao, Sun Jie, Zhang Fuyou. PCR Detection Method of Chicken Salmonella Enteritidis

1 Scope

This standard specifies the PCR detection method for molecular typing of Salmonella enteritidis. This standard is applicable to the PCR method detection of Salmonella enteritidis carried in chickens and their products of various ages.

2 Normative references

The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article Pieces. For undated reference documents, the latest version (including all amendments) is applicable to this document. GB 4789.4-2016 National Food Safety Standard Food Microbiological Inspection Salmonella Inspection GB/T 6682-2008 Analytical laboratory water specifications and test methods GB/T 28642-2012 Rapid detection method of Salmonella in feed polymerase chain reaction (PCR) method NY/T 541-2016 Technical Specifications for Collection, Storage and Transportation of Veterinary Diagnostic Samples

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 House-keepinggene A class of genes that are expressed in all types of cells in the physiology, pathology, and different developmental stages of an organism. Such genes are highly protected Shou, gene products are essential for maintaining the basic structure and metabolic functions of cells.

4 Abbreviations

The following abbreviations apply to this document. DNA. deoxyribonucleic acid (deoxyribonucleicacid) PCR. polymerase chain reaction (polymerase chain reaction)

5 Reagents and materials

Unless otherwise specified, all reagents used are of analytical grade. The experimental water meets the GB/T 6682-2008 Grade II water regulations. 5.1 LB liquid medium (see A.1 in Appendix A for the preparation method). 5.2 Buffered peptone water (BPW) pre-enrichment solution (see A.2 for the preparation method). 5.3 Selenite cystine (SC) enrichment solution (see A.3 for the preparation method). 5.4 Tetrathiosulfonate brilliant green (TTB) bacteria enrichment solution (see A.4 for the preparation method). 5.5 Xylose Lysine Deoxycholate (XLD) Agar D.3 (see A.5 for the preparation method). 5.6 TAE running buffer (see A.6 for the preparation method). 5.7 Positive and negative control. The positive control is the standard strain of Salmonella enteritidis (CVCC3377) and the standard strain of Salmonella pullorum ......

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