GB/T 38740-2020 English PDFUS$189.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 38740-2020: Laboratory animal - Method for examination of simian Marburg virus (MARV) Status: Valid
Basic dataStandard ID: GB/T 38740-2020 (GB/T38740-2020)Description (Translated English): Laboratory animal - Method for examination of simian Marburg virus (MARV) Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B44 Classification of International Standard: 65.020.30 Word Count Estimation: 10,161 Date of Issue: 2020-04-28 Date of Implementation: 2020-08-01 Quoted Standard: GB/T 6682; GB/T 18088; GB 19489 Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration Summary: This standard specifies the operation method for real-time fluorescent RT-PCR detection of simian Marburg virus. This standard applies to the detection of monkey Marburg virus. GB/T 38740-2020: Laboratory animal - Method for examination of simian Marburg virus (MARV)---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Laboratory animal--Method for examination of simian Marburg virus (MARV) ICS 65.020.30 B44 National Standards of People's Republic of China Laboratory animal monkey Marburg virus detection method 2020-04-28 release 2020-08-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed and managed by the National Laboratory Animal Standardization Technical Committee (SAC/TC281). This standard was drafted by. Hainan Entry-Exit Inspection and Quarantine Bureau Inspection and Quarantine Technology Center, People ’s Republic of China Fuzhou Customs, People ’s Republic of China Chengdu Customs of the Republic, Haikou Customs of the People's Republic of China, Jinan Customs of the People's Republic of China, Beijing Brain Science and Brain-like Research Center, Haikou Customs of the People's Republic of China, Kunming Customs of the People's Republic of China. The main drafters of this standard. Li Dandan, Zhang Tiyin, An Wei, Wang Suijia, Ling Zongshuai, Li Wenlong, Chen Pingya, Ai Jun. Laboratory animal monkey Marburg virus detection method1 ScopeThis standard specifies the operation method for real-time fluorescence RT-PCR detection of monkey Marburg virus. This standard applies to the detection of Marburg virus.2 Normative referencesThe following documents are essential for the application of this document. For dated references, only the dated version applies to this article Pieces. For the cited documents without date, the latest version (including all amendments) applies to this document. GB/T 6682 Analysis laboratory water specifications and test methods GB/T 18088 Entry and exit animal quarantine sampling GB 19489 General requirements for laboratory biosecurity3 Terms and definitionsThe following terms and definitions apply to this document. 3.1 Marburg virus A highly pathogenic virus belonging to the family of Filoviridae (Filoviridae), a single-stranded negative-strand RNA virus with a genome of approximately 19.1 kb, is known The largest negative-strand RNA virus. 3.2 Real-time quantitative polymerase chain reaction real-time quantitative reverse polymerase chainre- action; real-timeRT-PCR Reverse transcribe mRNA into cDNA and use it as a template for real-time fluorescence PCR amplification. Add a pair of primers to the PCR reaction system At the same time, a specific fluorescent probe is added.The probe is an oligonucleotide fragment, and a reporter fluorescent group and a Quenching fluorophores. When the probe is complete, the fluorescent signal emitted by the reporter group is absorbed by the quencher group; when PCR-specific amplification, the Taq enzyme The 5'-3 'exonuclease activity will degrade the probe, so that the reporter fluorescent group and the quenching fluorescent group are separated, so that the fluorescence monitoring system can receive To the fluorescence signal, the accumulation of the fluorescence signal and the PCR product formation are completely synchronized. 3.3 Noise The background fluorescence intensity of the fluorescence PCR instrument in a state where only a PCR tube and water are used. 3.4 Ct value cyclethresholdvalue The number of PCR cycles corresponding to the inflection point from baseline to exponential growth.4 AcronymsThe following abbreviations apply to this document. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 38740-2020_English be delivered?Answer: Upon your order, we will start to translate GB/T 38740-2020_English as soon as possible, and keep you informed of the progress. 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