GB/T 38571-2020 English PDF

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GB/T 38571-2020: Determination of alkaloids as plant secondary metabolites - Liquid chromatography-mass spectrometry
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 38571-2020	209	Add to Cart	3 days	Determination of alkaloids as plant secondary metabolites - Liquid chromatography-mass spectrometry	Valid

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Basic data

Standard ID: GB/T 38571-2020 (GB/T38571-2020)
Description (Translated English): Determination of alkaloids as plant secondary metabolites - Liquid chromatography-mass spectrometry
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A21
Classification of International Standard: 07.080
Word Count Estimation: 11,118
Date of Issue: 2020-03-31
Date of Implementation: 2020-03-31
Quoted Standard: GB/T 6682
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration
Summary: This standard specifies the method for the detection of plant secondary metabolite alkaloids by liquid chromatography-tandem mass spectrometer. This standard applies to (-)-scopolamine, scopolamine, aconitine, hypoconitine, neoaconitine, colchicine, atropine, strychnine, camptothecin, pilocarpine, podophyllin Determination of toxins.

GB/T 38571-2020: Determination of alkaloids as plant secondary metabolites - Liquid chromatography-mass spectrometry

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of alkaloids as plant secondary metabolites--Liquid chromatography-mass spectrometry ICS 07.080 A21 National Standards of People's Republic of China Detection of plant secondary metabolite alkaloid Liquid chromatography-mass spectrometry 2020-03-31 release 2020-03-31 Implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by China National Institute of Standardization. This standard was drafted by. Institute of Biology, China Institute of Testing Technology, Hebei Food Inspection Institute, China Institute of Standardization, Hebei Medical University, China Jiliang University. The main drafters of this standard. Zhang Yan, Fan Sufang, Ma Aijin, Feng Dejian, Ma Chunling, Wen Di, Cong Bin, Wang Juan, Lu Pin, Ye Zihong. Detection of plant secondary metabolite alkaloid Liquid chromatography-mass spectrometry

1 Scope

This standard specifies the method for the detection of plant secondary metabolite alkaloids by liquid chromatography-tandem mass spectrometry. This standard applies to (-)-scopolamine, papaverine, aconitine, hypoaconitine, neoaconitine, colchicine, atropine, strychnine, hi Determination of dendropine, pilocarpine, podophyllotoxin.

2 Normative references

The following documents are essential for the application of this document. For dated references, only the dated version applies to this article Pieces. For the cited documents without date, the latest version (including all amendments) applies to this document. GB/T 6682 Analysis laboratory specifications and test methods

3 Principle

After ultrasonic extraction and centrifugation, the samples were measured by liquid chromatography-tandem mass spectrometry and quantified by external standard method.

4 Reagents or materials

Unless otherwise specified, the reagents used are of analytical grade. The water is the first grade water specified in GB/T 6682. 4.1 10% (volume fraction) acetonitrile solution 10mL acetonitrile 90mL deionized water, mix well. 4.2 Formic acid Chromatographically pure. 4.3 0.1% (volume fraction) formic acid aqueous solution Accurately transfer 1.0mL of formic acid into a 1L volumetric flask, make up to volume with water, and mix well. 4.4 1% (volume fraction) formic acid-acetonitrile solution 1mL formic acid 99mL acetonitrile, mix well. 4.5 100mmol/L ammonium acetate solution Weigh 3.854g of ammonium acetate, dissolve in deionized water, and bring the volume to 500mL. 4.6 Ammonium acetate-ammonia buffer, pH9 Take 100mL100mmol/L ammonium acetate solution and adjust the pH to 9.0 ± 0.5 with ammonia water. ......

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