GB/T 38551-2020 English PDF

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 38551-2020	RFQ	ASK	3 days	Identification of plant varieties - MNP marker method	Valid

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Basic data

Standard ID: GB/T 38551-2020 (GB/T38551-2020)
Description (Translated English): Identification of plant varieties - MNP marker method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B04
Classification of International Standard: 65.020.01
Word Count Estimation: 330,332
Date of Issue: 2020-03-06
Date of Implementation: 2020-10-01
Quoted Standard: GB/T 3543.2; GB/T 6882; GB/T 19557.1; NY/T 2594
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration
Summary: This standard specifies the principles, reagents or materials, instruments and equipment, determination steps and result analysis of the polynucleotide polymorphism (MNP) labeling method for plant variety identification. This standard applies to rice (Oryza sativa L.), corn (Zea mays L.), soybean [Glycine max (Linn.) Merr.], cotton (Gossypium spp.), peanut (Arachis hypogaea L.), millet (Setaria italica ), watermelon (Citrullus lanatus), melon (Cucumis melo L.), cucumber (Cucumis sativus L.), wormwood (A

GB/T 38551-2020: Identification of plant varieties - MNP marker method

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Identification of plant varieties--MNP marker method ICS 65.020.01 B04 National Standards of People's Republic of China Plant variety identification MNP marker method Released on 2020-03-06 2020-10-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Contents

Foreword Ⅲ 1 Scope 1 2 Normative references 1 3 Terms and definitions 1 4 Principle 2 5 Reagents or materials 2 6 Equipment 2 7 Measurement step 2 7.1 Operational requirements 2 7.2 Sampling 2 7.3 DNA extraction 2 7.4 Multiplex PCR amplification and library construction 2 7.5 High-throughput sequencing 2 7.6 Sequencing data quality control 3 8 Results analysis 3 8.1 Calculation of results 3 8.2 Judgment of results 3 Appendix A (Normative Appendix) MNP Marker Primer 5 A.1 Rice MNP marker primer 5 A.2 Maize MNP marker primer 35 A.3 Soybean MNP primer 63 A.4 Cotton MNP marker primer 78 A.5 Peanut MNP primer 109 A.6 Millet MNP labeled primer 124 A.7 Watermelon MNP marker primer 148 A.8 Melon MNP primer 171 A.9 Cucumber MNP labeled primer 192 A.10 Wormwood MNP primer 208 A.11 Tomato MNP primer 218 A.12 Pepper MNP primer 235 A.13 Chinese cabbage MNP marker primer 260 A.14 Longan MNP primer 275 A.15 Litchi MNP primer 294 A.16 Kiwi MNP primer 312

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by China National Institute of Standardization. This standard was drafted by. Jianghan University, Wuhan Mingle Biotechnology Co., Ltd., China National Institute of Standardization, Ministry of Agriculture and Rural Science and Technology Development Center, Beijing Technology and Business University, Vegetable Research Center of Beijing Academy of Agriculture and Forestry Sciences, Hunan Hybrid Rice Research Center, South China Agricultural University, Zhangjiakou Academy of Agricultural Sciences, Shenzhen Huada Zhizao Technology Co., Ltd. The main drafters of this standard. Peng Hai, Fang Zhiwei, Li Lun, Ma Aijin, Zhou Junfei, Wen Changlong, Li Tiantian, Tang Hao, Chen Hong, Cui Yehan, Zhang Jianan, Jia Yingmin, Xu Na, Song Shufeng, Hu Meixia, Fu Xiqin, Zhao Zhihai, Liang Yong, Xu Zhenjiang, Gao Lifen, Chen Lihong, Han Ruixi, Zhang Die, Zhang Jing, Yu Jinwen. Plant variety identification MNP marker method

1 Scope

This standard specifies the principles, reagents or materials, instruments and equipment, and determination steps of the polynucleotide polymorphism (MNP) labeling method for plant variety identification Steps, results analysis. This standard applies to rice (Oryzasativa L.), corn (Zeamays L.), soybean [Glycinemax (Linn.) Merr.], Cotton Flowers (Gossypiumspp.), Peanuts (Arachishypogaea L.), millet (Setariaitalica), watermelon (Citrulluslanatus), sweet Melon (CucumismeloL.), Cucumber (CucumissativusL.), Wormwood (Artemisiaargyi), tomato (Lycopersiconesculen- tum Mil.), pepper (Capsicumannuum L.), cabbage [Brassicapekinensis (Lour.) Rupr.], longan (Dimocarpus LonganLour.), litchi (Litchichinensis Sonn.), kiwi (ActinidiaChinensis) original variety identification, substantial faction Raw variety identification and variety authenticity identification. The identification of other plant varieties can refer to this standard.

2 Normative references

The following documents are essential for the application of this document. For dated references, only the dated version applies to this article Pieces. For the cited documents without date, the latest version (including all amendments) applies to this document. GB/T 3543.2 Regulations for inspection of crop seeds GB/T 6882 Acoustic sound pressure method for determination of noise source sound power level and sound energy level Precision methods for anechoic and semi-anechoic rooms GB/T 19557.1 General guidelines for testing specificity, consistency and stability of new plant varieties NY/T 2594 General rules of DNA molecular markers for plant variety identification

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Polynucleotide polymorphism; MNP Sequence polymorphism caused by multiple nucleotides at the genomic level. 3.2 Essentially derived variety; EDV Derived from the original variety or re-derived from the substantial variety of the original variety, and the genotype or gene of the original variety is retained The basic characteristics produced by the type combination, but there are obvious differences from the original varieties. 3.3 Degree of variability Alien plants (atypical plants) account for the percentage of the total observed plants. 3.4 Average coverage multiple The ratio of the number of sequenced fragments to the number of labeled sites aligned to the labeled sites. 3.5 Detected markers At least one allelic type has 20 or more marker sites supported by sequencing fragments. ......

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