GB/T 38490-2021 English PDF

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GB/T 38490-2021: Determination of high-throughput adaptive evolution for microorganisms - Microfluidic chip method
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Basic data

Standard ID: GB/T 38490-2021 (GB/T38490-2021)
Description (Translated English): Determination of high-throughput adaptive evolution for microorganisms - Microfluidic chip method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A21
Word Count Estimation: 5,535
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 38490-2021: Determination of high-throughput adaptive evolution for microorganisms - Microfluidic chip method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of high-throughput adaptive evolution for microorganisms -- Microfluidic chip method ICS 07:080 CCSA21 National Standards of People's Republic of China Microbial high-throughput adaptive evolution assay Microfluidic chip method Published on 2021-12-31 2022-07-01 Implementation State Administration for Market Regulation Released by the National Standardization Administration

foreword

This document is in accordance with the provisions of GB/T 1:1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules of Standardization Documents" drafted: Please note that some content of this document may be patented: The issuing agency of this document assumes no responsibility for identifying patents: This document is proposed and managed by China National Institute of Standardization: This document was drafted by: Tsinghua University, Luoyang Huaqing Tianmu Biotechnology Co:, Ltd:, China National Institute of Standardization: The main drafters of this document: Zhang Chong, Xing Xinhui, Jian Xingjin, Li Mei, Wang Liyan, Guo Xiaojie, Zhang Lele, Ma Aijin: Microbial high-throughput adaptive evolution assay Microfluidic chip method

1 Scope

This document specifies a method for measuring the high-throughput adaptive evolution of microorganisms using a microfluidic chip method: This document applies to the high-throughput adaptation of culturable microorganisms in single-cell morphology that does not produce surfactants using the microfluidic chip method Evolutionary Determination:

2 Normative references

The contents of the following documents constitute essential provisions of this document through normative references in the text: Among them, dated citations documents, only the version corresponding to that date applies to this document; for undated references, the latest edition (including all amendments) applies to this document: GB/T 6682 Analysis Laboratory Water Specifications and Test Methods

3 Terms and Definitions

The following terms and definitions apply to this document: 3:1 adaptive evolution Under laboratory conditions, the microorganisms are continuously subcultured in a specific environment to simulate the long-term effect of microorganisms on the environment: adaptation process: 3:2 fitness Quantitative characteristics of organisms or groups of organisms to adapt to the environment, is to evaluate the adaptability of various characteristics of organisms, and the evolution of An indicator of the ability to continue to pass on to future generations in the process:

4 Principles

Micro-droplets with culture medium encapsulating the bacteria are generated on the micro-organism culture chip, and the micro-droplets are reciprocated in the main pipe of the chip to make the micro-droplets The microbes grow and multiply on the chip, and the medium is continuously supplemented and replaced through the droplet segmentation and fusion operation, and the continuous passage of microorganisms is realized: Realize the adaptive evolution of microorganisms to specific culture conditions, and obtain strains with increased growth rate or increased tolerance to specific environments:

5 Reagents and Materials

Unless otherwise specified, only analytical grade reagents were used: 5:1 Water GB/T 6682, Grade 1 water: ......

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