GB/T 36433-2018 English PDF

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GB/T 36433-2018: Textiles -- DNA quantitative analysis of cashmere and wool mixture -- Fluorescence PCR method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 36433-2018	199	Add to Cart	3 days	Textiles -- DNA quantitative analysis of cashmere and wool mixture -- Fluorescence PCR method	Valid

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Basic data

Standard ID: GB/T 36433-2018 (GB/T36433-2018)
Description (Translated English): Textiles -- DNA quantitative analysis of cashmere and wool mixture -- Fluorescence PCR method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: W04
Classification of International Standard: 59.080.01
Word Count Estimation: 10,179
Date of Issue: 2018-06-07
Date of Implementation: 2019-01-01
Regulation (derived from): National Standards Announcement No. 9 of 2018
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36433-2018: Textiles -- DNA quantitative analysis of cashmere and wool mixture -- Fluorescence PCR method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Textiles--DNA quantitative analysis of cashmere and wool mixture--Fluorescence PCR method ICS 59.080.01 W04 National Standards of People's Republic of China Textile cashmere and sheep wool Quantitative analysis of mixture DNA by fluorescence PCR Published on.2018-06-07 2019-01-01 Implementation National Market Supervision Administration China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed by the China Textile Industry Federation. This standard is under the jurisdiction of the National Textile Standardization Technical Committee (SAC/TC209). This standard was drafted by Shanghai Entry-Exit Inspection and Quarantine Bureau and China Textile Standard Inspection & Certification Co., Ltd. The main drafters of this standard. Fei Jing, Xie Yuman, Rui Yanghua, Si Ying, Tang Minfeng, Lu Weimin. Textile cashmere and sheep wool Quantitative analysis of mixture DNA by fluorescence PCR

1 Scope

This standard specifies the quantitative DNA detection method for the determination of cashmere and sheep wool in textiles using fluorescent PCR. This standard applies to the detection of two components of cashmere and sheep wool in textile blends. This standard does not apply to recycled, stripped cashmere and sheep wool fibers, and Angora wool.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. GB/T 6682 Analysis Laboratory Water Specifications and Test Methods GB/T 16988 Determination of Mixture of Special Animal Fibers and Sheep Wool General requirements and definitions for GB/T 19495.1 genetically modified products GB/T 19495.2 Laboratory Testing Requirements for Genetically Modified Products

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 Polymerase chain reaction polymerase chain reaction; PCR Under the catalysis of DNA polymerase, using the parent strand DNA as a template and the specific primers as the starting points, 4 kinds of single nucleotides (dNTP) are The substrate, through a denaturing, annealing, and extended cyclic reaction, causes a very small amount of specific fragments of DNA to be amplified millions of times in a matter of hours. In vitro amplification of DNA by chain reaction. 3.2 Real-time fluorescence PCR Fluorescence groups are added to the PCR reaction system to monitor the entire PCR process in real-time using fluorescence signal accumulation and then pass the curve to unknown Template for quantitative or qualitative analysis of DNA amplification methods. 3.3 Primer primer In the nucleic acid synthesis reaction, a small piece of single-stranded DNA that functions as a starting point for the extension of each polynucleotide strand and acts Or RNA. 3.4 TaqMan probe TaqManprobe One fluorophore is attached to the 5' end of the probe and the quencher is the 3' end of the oligonucleotide. Note. A specific fluorescent probe is added at the same time that a pair of primers is added during PCR amplification. When the probe is intact, the fluorescence signal emitted by the reporter group is quenched. The group absorbs; during PCR amplification, the Taq enzyme's 5′-3′ exonuclease activity degrades the probe, causing the reporter and quencher fluorophores to separate. The fluorescence monitoring system can receive the fluorescence signal, that is, one fluorescent molecule is formed for each DNA strand to be amplified, and the fluorescence signal is accumulated. ......

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