GB/T 36194-2018 English PDF

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GB/T 36194-2018: Detection method of goldfish haematopoietic necrosis virus
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Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 36194-2018	179	Add to Cart	3 days	Detection method of goldfish haematopoietic necrosis virus	Valid

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Basic data

Standard ID: GB/T 36194-2018 (GB/T36194-2018)
Description (Translated English): Detection method of goldfish haematopoietic necrosis virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 65.020.30
Word Count Estimation: 9,954
Date of Issue: 2018-05-14
Date of Implementation: 2018-12-01
Regulation (derived from): National Standards Announcement No. 6 of 2018
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 36194-2018: Detection method of goldfish haematopoietic necrosis virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Goldfish hematopoietic organ necrosis virus detection method) ICS 65.020.30 B41 National Standards of People's Republic of China Goldfish hematopoietic organ necrosis virus detection method Published on.2018-05-14 2018-12-01 implementation State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. Publication of this document The organization is not responsible for identifying these patents. This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic This standard is under the jurisdiction of the National Aquaculture Standardization Technical Committee (SAC/TC156). This standard was drafted. National Aquatic Technology Extension Station, China Academy of Inspection and Quarantine, Beijing Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Li Qing, Wang Na, Gu Qiang, Zhang Wei, Jing Hongli, Wu Shaoqiang, Zhang Lifeng, Jiang Yulin, Chen Haonan. Goldfish hematopoietic organ necrosis virus detection method

1 Scope

This standard specifies the PCR detection, fluorescent PCR detection and comprehensive determination of goldfish hematopoietic necrosis virus (herpesvirus type 2). method. This standard applies to the identification of goldfish hematopoietic necrosis virus, and is used in the epidemic of related diseases caused by goldfish hematopoietic necrosis virus. Disease investigation, diagnosis, quarantine and monitoring.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 18088 entry and exit animal quarantine sampling SC/T 7103 Technical specification for quarantine sampling of aquatic animals

3 Abbreviations

The following abbreviations apply to this document. CyHV. herpes simplex virus (cyprinidherpesvirus) CyHV-2. herpes simplex virus type 2 (cyprinidherpesvirus2) GFHNV. goldfish haematopoieticnecrosisvirus dNTP. deoxy-ribonucleoside triphosphate Bp. basepair Ct. cycle threshold (cyclethreshold) CTAB. cetyltrimethylammonium bromide EDTA. ethylenediaminetetraacetic acid Tris. tris(hydroxymethyl)aminomethane [tris(hydroxymethyl)aminomethane]

4 reagents and materials

Analytically pure reagents were used unless otherwise stated. 4.1 Water. It should meet the specifications of the first grade water in GB/T 6682. 4.2 Taq DNA polymerase. 5 U/μL. Store at -20 °C to avoid repeated freezing and thawing or drastic changes in temperature. 4.3 dNTP. Contains dMTP, dGTP, dATP, dTTP each 10mmoL/L, stored at -20 °C. 4.4 Primers. The concentration was 10 μmol/L and stored at -20 °C. a) CyHV primer 1) CyHVpol-F. 5'-CCCAGCAACATGTGCGACGG-3' 2) CyHVpol-R. 5'-CCGTARTGAGAGTTGGCGCA-3' ......

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