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GB/T 35874-2018 English PDF

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GB/T 35874-2018: Technical regulation for evaluation of sugarcane resistance to smut (Sporisorium scitaminea Syd.)
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GB/T 35874-2018199 Add to Cart 3 days Technical regulation for evaluation of sugarcane resistance to smut (Sporisorium scitaminea Syd.) Valid

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Basic data

Standard ID: GB/T 35874-2018 (GB/T35874-2018)
Description (Translated English): Technical regulation for evaluation of sugarcane resistance to smut (Sporisorium scitaminea Syd.)
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 10,165
Date of Issue: 2018-02-06
Date of Implementation: 2018-09-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 35874-2018: Technical regulation for evaluation of sugarcane resistance to smut (Sporisorium scitaminea Syd.)


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Technical regulation for evaluation of sugarcane resistance to smut (Sporisorium scitaminea Syd.) ICS 65.020.01 B16 National Standards of People's Republic of China Sugarcane smut resistance identification technical specification (Sporisorium scitamineaSyd.) 2018-02-06 released 2018-09-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard proposed by the China National Institute of Standardization and centralized. This standard was drafted. Guangxi Zhuang Autonomous Region Academy of Agricultural Sciences, Guangxi Zhuang Autonomous Region Standards Institute of Technology, China Standardization Research Institute, Chinese Academy of Agricultural Sciences Sugarcane Research Center. The main drafters of this standard. Zeng Yunqing, Wang Quan Yong, Zhou Zhiquan, Tang its exhibition, Jinjian Ying, Gao Yi Jing, Luo Chaoyan, Wei Shan Fu, Wang Huan, Xi Xingjun, Yang Feng, Huang Kun, Lin Jianye, Lin Cuixia, Peng Yingzhi, Wu Min, Fang Wei Kuan, Guo Chenlin, the first overseas Chinese, He Meiling, Luo Kun. Sugarcane smut resistance identification technical specification

1 Scope

This standard specifies the sugarcane smut resistance identification methods and disease resistance evaluation criteria. This standard applies to sugarcane varieties, lines and germplasm resources appraisal and evaluation of sugarcane smut resistance.

2 Terms and definitions

The following terms and definitions apply to this document. 2.1 Sugarcane smut sugarcanesmutdisease The main disease caused by the infection of Sporisorium scitamineaSyd., The typical symptoms of the disease Long black black whip appears. 2.2 Control variety controlvariety A standard control variety of known resistance compares the resistance performance of the clones tested. 2.3 Acupuncture dipping inoculation pin-prickdippinginoculationmethod Inoculation of sugarcane buds with insect needles and immersion in spore suspension of spores of gibberelliasis.

3 pathogen spore suspension preparation

3.1 Collection and preservation of pathogens Collection of spores of gizzards should be carried out on a sunny day in the field to collect different sugarcane varieties of unswept powder smut black whip, remove the outer layer I attachments, put it into a clean kraft paper bag fold seal. 38 ℃ drying 72h, 0.250 sieve aperture (60 mesh sieve) sealed with a paper bag Set low temperature (0 ℃ ~ 4 ℃) to save spare. 3.2 vitality test The collected spores were inoculated into the medium (1% agar 1% sucrose) and incubated at (28 ± 1) ℃ for 24 h. 60 times microscope observation of spores Seed germination (a field of about 40 spores), germination rate of 90.0% or more for the vaccination requirements. 3.3 pathogen spore suspension preparation The viable spores tested qualified spores plus diluted hydrochloric acid (with 1N hydrochloric acid mother liquor diluted to 0.2%) dubbed 5 × 106/mL hanging The liquid to be used.

4 resistance identification

4.1 resistance identification nursery Identification nursery should be located in the greenhouse or shed. Temperature range. 15 ℃ ~ 50 ℃; humidity range. 30% ~ 80%.
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