GB/T 3543.11-2025 English PDFUS$679.00 · In stock
Delivery: <= 5 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 3543.11-2025: Rules for agricultural seed testing - Part 11: Varietal quality - Varietal genuineness identification testing Status: Valid
Basic dataStandard ID: GB/T 3543.11-2025 (GB/T3543.11-2025)Description (Translated English): Rules for agricultural seed testing - Part 11: Varietal quality - Varietal genuineness identification testing Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B00 Classification of International Standard: 65.020.20 Word Count Estimation: 34,361 Date of Issue: 2025-02-28 Date of Implementation: 2025-09-01 Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration GB/T 3543.11-2025: Rules for agricultural seed testing - Part 11: Varietal quality - Varietal genuineness identification testing---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. GB/T 3543.11-2025 English version. Rules for agricultural seed testing - Part 11.Varietal quality - Varietal genuineness identification testing ICS 65.020.20 CCSB00 National Standard of the People's Republic of China Partially replaces GB/T 3543.5-1995 Crop Seed Inspection Procedures Part 11.Variety quality and authenticity identification Part 11.Varietalquality-Varietalgenuinenessidentificationtesting Released on 2025-02-28 2025-09-01 Implementation State Administration for Market Regulation The National Standardization Administration issued ForewordThis document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents" Drafting. This document is part 11 of GB/T 3543 “Regulations for the inspection of crop seeds”. GB/T 3543 has been published in the following parts. --- Part 1.General; --- Part 2.Sampling; --- Part 3.Seeding quality and purity analysis; --- Part 4.Seeding quality germination test; --- Part 5.Variety quality and purity identification; --- Part 6.Determination of moisture content of sowing mass; --- Other items inspection; --- Part 8.Determination of sowing quality and thousand-grain weight; --- Part 9.Determination of sowing quality and viability; --- Part 10.Determination of seeding quality and vitality; --- Part 11.Variety quality and authenticity identification; --- Part 12.Determination of variety quality of genetically modified seeds. This document partially replaces GB/T 3543.5-1995 "Crop Seed Inspection Procedure for Authenticity and Variety Purity Identification" In the part on sexual identification, compared with GB/T 3543.5-1995, in addition to structural adjustments and editorial changes, the main technical changes are as follows. --- Deleted the content of variety purity identification (see.1995 edition); --- Changed some of the terms and definitions of authenticity of varieties (see Chapter 3, Chapter 3 of the.1995 edition); --- Added general requirements for identification content (see Chapter 4), DNA molecular testing (see 5.1) and plot planting identification (see 5.2); --- Deleted the morphological identification method (see 6.2.1 of the.1995 edition), the rapid determination method (see 6.2.2 of the.1995 edition), the seedling identification method (see 6.3 of the.1995 edition); --- Changed the identification procedure (see Chapter 6, Chapter 6 of the.1995 edition); --- Changed the calculation and presentation of results (see Chapter 7, Chapter 7 of the.1995 edition); --- Changed the result report (see Chapter 8, Chapter 8 of the.1995 edition); --- Added SSR primer information for authenticity identification of major crop varieties (see Appendix A), SSR molecular marker identification procedures (see Appendix B) and SNP molecular marker identification procedures (see Appendix C). Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents. This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China. This document is under the jurisdiction of the National Technical Committee on Standardization of Crop Seeds (SAC/TC37). This document was drafted by. National Agricultural Technology Extension Service Center, Beijing Academy of Agricultural and Forestry Sciences, China National Rice Research Institute, Sichuan Provincial Seed Station, Cotton Research Institute, Chinese Academy of Agricultural Sciences. The main drafters of this document are. Jin Fang, Wang Fengge, Pang Binshuang, Xu Qun, Liu Lihua, Yi Hongmei, Ge Jianrong, Li Hongbo, Tian Hongli, Wei Xinghua, Liu Yangna, Li Yu, Fan Baodan, Jin Shiqiao, Sun Quan, Ren Xuezhen, Fu Shouyang. The previous versions of this document and the documents it replaces are as follows. ---First issued in 1983 as GB 3543-1983; ---First revised in.1995 to GB/T 3543.5-1995; --- This is the second revision, partially replacing GB/T 3543.5-1995.IntroductionSeed quality has many verifiable cultivation values, forming the currently recognized sowing quality, variety quality and health quality. Among them, sowing quality mainly reflects the impact of seed appearance quality on seed sowing, and is divided into physical Quality and physiological quality. The most commonly used physical quality is cleanliness, dryness, fullness, uniformity and other characteristics, such as water Physiological quality mainly reflects the vitality of seeds, especially under suitable environmental control conditions. The structural function and developmental capacity of seedlings under most conditions, that is, the ability to normally germinate and grow into plants under most conditions. The main indicators of physiological quality are development Seed variety quality (varietal quality) is also called genetic quality (genetic quality), which mainly reflects The characteristics of the genetic background of crop seeds and the degree of consistency of genetic background. At present, the indicators for measuring seed variety quality include variety authenticity, The seed quality mainly reflects the seed health status, whether there is Pathogenic organisms, such as fungi, bacteria and viruses, and animal pests, including nematodes and insects, may also involve physiological conditions, such as trace element deficiencies. GB/T 3543 "Inspection Procedures for Crop Seeds" is the basic standard for seed inspection methods, which consists of general principles, sampling, sowing quality, variety quality, etc. It is composed of the following 12 parts. quantity, health quality, etc., which are intended to provide inspection basis for the quality requirements of the above three types of seeds. part. --- Part 1.General. The purpose is to establish the general and overall requirements for each part of the inspection procedures for crop seeds and to govern the inspection procedures. Each part of the program. --- Part 2.Sampling. The purpose is to clarify the methods of sampling from seed batches, sub-sampling for testing and preservation of samples. program. --- Part 3.Seeding quality purity analysis. The purpose is to clarify the method for determining the net seed content in crop seed samples program. --- Part 4.Seeding quality germination test. The purpose is to clarify the test method and procedures for the germination of crop seed samples. --- Part 5.Variety quality and purity identification. The purpose is to determine the percentage of seeds of this variety in a seed sample. Methods and procedures. --- Part 6.Determination of moisture content in seeding mass. The purpose is to clarify the method and procedure for moisture content of crop seed samples. --- Part 7.Other items of inspection. The purpose is to clarify the method and procedures for seed sample health. --- Part 8.Determination of 1000-seed weight of sowing quality. The purpose is to clarify the method and procedure for determining the weight of 1000 seeds per seed sample. --- Part 9.Determination of sowing quality viability. The purpose is to clarify the method and procedure for determining the viability of seed samples. --- Part 10.Determination of seed quality vitality. The purpose is to clarify the method and procedure for determining the vitality of seed samples. --- Part 11.Variety quality and authenticity identification. The purpose is to clarify the identification methods and procedures for variety authenticity. --- Part 12.Determination of genetically modified seeds for variety quality. The purpose is to clarify the composition, genetic purity and gene authenticity of genetically modified seeds. Procedure for determination of authenticity. This document is the "Variety Authenticity Identification" part of variety quality. This document follows international common practice and is based on the marker site (primer) With consistency as the focus, molecular testing methods are standardized. Variety authenticity is an important indicator of variety quality and is widely used in seed trade to protect the legitimate rights and interests of all parties and to protect the seed industry. Supervision and management of production and operation activities. Crop Seed Inspection Procedures Part 11.Variety quality and authenticity identification1 ScopeThis document specifies the content, general requirements, identification procedures, and result presentation requirements for authenticity identification of crop seed varieties. This document applies to the inspection of crop seeds.2 Normative referencesThe contents of the following documents constitute the essential clauses of this document through normative references in this document. For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to This document. GB/T 3543.1 Inspection procedures for crop seeds Part 1.General GB/T 3543.2 Inspection procedures for crop seeds Part 2.Sampling3 Definitions and abbreviationsThe following terms and definitions apply to this document. 3.1 Definition of terms 3.1.1 Variety identification content 3.1.1.1 Variety verification By comparing with the standard sample with the corresponding variety name, determine whether the variety name marked on the sample for inspection is authentic. 3.1.1.2 Variety identification The authenticity of the sample variety is verified by comparing it with the crop variety fingerprint data comparison platform or by comparing the planting in the field plots. Name or identity with other varieties. 3.1.1.3 variety trait detection After testing or screening, the sample to be tested is identified as having specific traits brought about by genetic modification or gene editing. 3.1.2 DNA molecular detection method 3.1.2.1 DNA molecular tests Based on the detection of nucleic acid molecules, the quality of varieties can be identified by comparing the results of DNA sequences of different samples or different individuals. Determined way. Note. The authenticity of the variety is detected by comparing the similarities and differences in DNA sequences between different sample groups. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 3543.11-2025_English be delivered?Answer: Upon your order, we will start to translate GB/T 3543.11-2025_English as soon as possible, and keep you informed of the progress. The lead time is typically 3 ~ 5 working days. 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