GB/T 34800-2017 English PDF

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GB/T 34800-2017: Detection method of activity and impurity of proteinase K
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 34800-2017	119	Add to Cart	3 days	Detection method of activity and impurity of proteinase K	Valid

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Basic data

Standard ID: GB/T 34800-2017 (GB/T34800-2017)
Description (Translated English): Detection method of activity and impurity of proteinase K
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A21
Classification of International Standard: 07.080
Word Count Estimation: 6,668
Date of Issue: 2017-11-01
Date of Implementation: 2018-05-01
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 34800-2017: Detection method of activity and impurity of proteinase K

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection method of activity and impurity of proteinase K ICS 07.080 A21 National Standards of People's Republic of China Proteinase K enzyme activity and impurity detection methods Posted.2017-11-01 2018-05-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard proposed by the China National Institute of Standardization and centralized. This standard was drafted. Dongying Ruida Biological Technology Co., Ltd., China National Institute of Standardization, China Biotechnology Development Center, Hebei Agricultural Industry University, Zhejiang Gongshang University, Hefei University of Technology, Exit Inspection and Quarantine of Hebei Province, Quanzhou Institute of Standardization, Beijing Food Institute of Science, Hebei Institute of Food Inspection, Henan University. The main drafters of this standard. Fang Cunlin, Ma Ai Jin, Su Yue, Sun Records, Wang Yanbo, Zheng Lei, Liu Daoliang, Lin Qingshan, Sun Yong, Yun Zhenyu, Fu Linglin, Zhao Lin, Wu Qi, Zhou Wei, Kang Arts, Chen Xinkai. Proteinase K enzyme activity and impurity detection methods

1 Scope

This standard specifies the protease K detection principle, equipment and apparatus, the main reagents, analysis steps and results analysis. This standard applies to biochemical reagents Proteinase K product production and testing.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods GB/T 34222 ribonuclease activity test method GB/T 34801 DNAzyme activity assay

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Protease K proteinaseK A serine protease that hydrolyzes native keratin. Note. By product form is divided into solid and liquid. 3.2 Protease K enzyme activity unit proteinaseKactivityunit Hydrolysis of bovine hemoglobin within 1 min at 57 ° C and pH 8.0 yielded an enzyme amount of 1 μmol of phenolic amino acids. Note. An enzyme activity unit to U said.

4 principle

Proteinase K catalyzes the cleavage of the carboxyl-terminal peptide bonds of aliphatic and aromatic amino acids. It is at 57 ° C and pH8.0 conditions, Hydrolysis of bovine hemoglobin substrate produces phenolic-containing amino acids; under basic conditions, Folin reagent can be reduced to form molybdenum blue and tungsten Blue, the color depth is proportional to the content of phenolic amino acids. By measuring the absorbance at 680 nm, a phenylamino group produced by hydrolysis is obtained The amount of acid, and then calculate the activity of protease K enzyme.

5 equipment and appliances

5.1 constant temperature water bath. Accuracy of ± 0.1 ℃. 5.2 pH meter. Accuracy of 0.1pH units. 5.3 spectrophotometer wavelength range 380nm ~ 780nm, the absorbance value accurate to 0.001. 5.4 1cm cuvette. ......

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