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GB/T 34757-2017 English PDF

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US$279.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 34757-2017: Porcine epidemic diarrhoea -- RT-PCR for detecting virus acid
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 34757-2017	279	Add to Cart	3 days	Porcine epidemic diarrhoea -- RT-PCR for detecting virus acid	Valid

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Basic data

Standard ID: GB/T 34757-2017 (GB/T34757-2017)
Description (Translated English): Porcine epidemic diarrhoea -- RT-PCR for detecting virus acid
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 14,167
Date of Issue: 2017-11-01
Date of Implementation: 2018-05-01
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 34757-2017: Porcine epidemic diarrhoea -- RT-PCR for detecting virus acid

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Porcine epidemic diarrhea-RT-PCR for detecting virus acid ICS 11.220 B41 National Standards of People's Republic of China Porcine epidemic diarrhea virus RT-PCR detection method Posted.2017-11-01 2018-05-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Animal Husbandry Standardization Technical Committee (SAC/TC181) centralized. This standard drafting unit. Northeast Agricultural University, China Animal Health and Epidemiology Center, Henan Animal Husbandry School of Economics, Henan Agriculture the University. The main drafters of this standard. Li a, Shao Satellite, Wei Rong, Song Jiande, Jiang Yanping, Yuan Liping, Tang Lijie, Joe pay, Cui Wen, Xu Yaohui, Wu Faxing, Zhang Zhi, Sun Yingxue, Wei Zhanyong, Li Xiaocheng. Porcine epidemic diarrhea virus RT-PCR detection method

1 Scope

This standard specifies the swine epidemic diarrhea virus RT-PCR detection equipment, reagents, sample collection and processing, RT-PCR process Order, electrophoresis and determination of the technical requirements. This standard applies to rapid detection of porcine swine clinical samples (pig intestine and fresh faeces) or cell culture pig epidemic diarrhea virus nucleic acid.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this edition file. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods

3 Abbreviations

The following abbreviations apply to this document. AMV. Avianmyoblastosis virus bp. basepair DEPC. Diethylpyrocarbonate DNA. Deoxyribonucleic acid dNTPs.4 deoxyribonucleosidetriphosphates PBS. Phosphate-bufferedsalinebuffer RNA (ribonucleic acid) RT-PCR. Reverse transcription-polymerase chain reaction (reversetranscriptase-polymerasechainreaction) RRI.RNase inhibitor (rnaseribonucleaseinhibitor) Taq enzyme Taq DNA polymerase (Taq DNA polymerase)

4 instruments

4.1 PCR amplification instrument. 4.2 high-speed desktop refrigerated centrifuge can be controlled temperature to 4 ℃, centrifuge speed up to 12000r/min above. 4.3 tissue grinder or mortar. 4.4 refrigerator 2 ℃ ~ 8 ℃ refrigerator and ultra-low temperature refrigerator below -70 ℃. 4.5 nucleic acid electrophoresis apparatus and horizontal electrophoresis tank. 4.6 Micropipette range 0.2μL ~ 2μL, 1μL ~ 10μL, 10μL ~ 100μL, 20μL ~.200μL and 100μL ~ 1000 μL pipettes and equipped with tips that match the pipettes. 4.7 autoclave. 4.8 Gel imaging system (or UV transilluminator). ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 34757-2017_English be delivered?

Answer: Upon your order, we will start to translate GB/T 34757-2017_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 34757-2017_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 34757-2017_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.