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GB/T 34748-2017 English PDF

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US$279.00 · In stock
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GB/T 34748-2017: Genetic diversity analysis on aquatic germplasm by genomic DNA microsatellite markers
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 34748-2017	279	Add to Cart	3 days	Genetic diversity analysis on aquatic germplasm by genomic DNA microsatellite markers	Valid

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Basic data

Standard ID: GB/T 34748-2017 (GB/T34748-2017)
Description (Translated English): Genetic diversity analysis on aquatic germplasm by genomic DNA microsatellite markers
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B50
Classification of International Standard: 65.150
Word Count Estimation: 14,126
Date of Issue: 2017-11-01
Date of Implementation: 2018-05-01
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 34748-2017: Genetic diversity analysis on aquatic germplasm by genomic DNA microsatellite markers

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Genetic diversity analysis on aquatic germplasm by genomic DNA microsatellite markers ICS 65.150 B50 National Standards of People's Republic of China Microsatellite Analysis of Genomic DNA from Aquatic Germplasm Resources Genetic diversity of aneuploidy mmplasmbygenomicDNAmicrosatelite markers Posted.2017-11-01 2018-05-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of this document may be patentable. The issuing agencies of this document do not bear the responsibility of identifying these patents. This standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Aquatic Standardization Technical Committee Freshwater Culture Sub-Technical Committee (SAC/TC156/SC1) centralized. This standard drafting unit. Chinese Academy of Fishery Sciences Heilongjiang Institute of Fisheries. The main drafters of this standard. Kuang Friendship, Sun Xiaowen, Zheng Xianhu, Liang Liqun. Microsatellite Analysis of Genomic DNA from Aquatic Germplasm Resources

1 Scope

This standard specifies the reagents and materials, instruments and equipment, analysis steps and number of microsatellite analysis of aquatic germplasm resources genomic DNA According to analysis. This standard applies to the microsatellite analysis of genomic DNA of aquatic germplasm resources.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 18654.15 farmed fish germplasm testing Part 15. RAPD analysis

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Microsatellites microsatellites Short tandem repeats consisting of two or more nucleotides in the genome of the eukaryotic genome. 3.2 Microsatellite analysis of microsatellites The microsatellite markers were used to amplify the genomic DNA. After the products were detected by gel electrophoresis, the electrophoresis patterns were analyzed and their In the implication of genetic polymorphism information.

4 Reagents and materials

Except for the reagents and materials specified in GB/T 18654.15, the following reagents and materials are required by this standard. In points unless otherwise stated Only the reagents identified as analytically pure and double-distilled water or de-ionized water or rather pure water are used in the analysis. 4.1 Deionized ultrapure water. Autoclave. 4.2 TEMED. Store at 4 ° C in refrigerator. 4.3 Microsatellite-labeled primer. Preparation method in line with Appendix A. 4.4 Acrylamide. DNA sequencing grade. 4.5 methylene bisacrylamide DNA sequencing grade. 4.6 urea. 4.7 Deionized formamide. 4.8 polyacrylamide gel storage solution, preparation method in accordance with Appendix A. 4.9 10% (mass ratio) of ammonium persulfate (APS) solution. Preparation method in accordance with Appendix A. 4.10 polyacrylamide gel fixing solution, preparation method in accordance with Appendix A. 4.11 polyacrylamide gel staining solution, preparation method in accordance with Appendix A. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 34748-2017_English be delivered?

Answer: Upon your order, we will start to translate GB/T 34748-2017_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 34748-2017_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 34748-2017_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.