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GB/T 34745-2017 English PDF

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US$179.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 34745-2017: Porcine circovirus type 2 -- Method of detecting virus by SYBR Green Ⅰ real-time PCR
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 34745-2017	179	Add to Cart	3 days	Porcine circovirus type 2 -- Method of detecting virus by SYBR Green Ⅰ real-time PCR	Valid

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Basic data

Standard ID: GB/T 34745-2017 (GB/T34745-2017)
Description (Translated English): Porcine circovirus type 2 -- Method of detecting virus by SYBR Green �� real-time PCR
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 9,974
Date of Issue: 2017-11-01
Date of Implementation: 2018-05-01
Regulation (derived from): National Standard Announcement 2017 No. 29
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 34745-2017: Porcine circovirus type 2 -- Method of detecting virus by SYBR Green Ⅰ real-time PCR

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Porcine circovirus type 2-Method of detecting virus by SYBR Green I real-time PCR ICS 11.220 B41 National Standards of People's Republic of China Porcine circovirus type 2 virus SYBRGreen I real-time Fluorescent Quantitative PCR Detection Method Posted.2017-11-01 2018-05-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Animal Husbandry Standardization Technical Committee (SAC/TC181) centralized. This standard drafting unit. Henan Agricultural University, China Animal Health and Epidemiology Center. The main drafters of this standard. Wei Zhan Yong, Zheng Lanlan, Shao Satellite, Hu Hui, Chen Hongying, Wang Yabin, Wang Xuebing, Cui Baoan, Kouanan. Porcine circovirus type 2 virus SYBRGreen I real-time Fluorescent Quantitative PCR Detection Method

1 Scope

This standard specifies the SYBRGreen Ⅰ real-time fluorescent quantitative PCR detection of porcine circovirus type 2 technical requirements. This standard for the detection of porcine circovirus type 2 nucleic acid for rapid detection of porcine circovirus type 2 in clinical samples.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods

3 Abbreviations

The following abbreviations apply to this document. Ct value. CycleThreshold DNA. Deoxyribonucleic Acid EDTA. Ethylenediamine tetraacetic acid (EthylenediaminetetraaceticAcid) PBS. Phosphate buffer solution (PhosphateBufferSaline) PCR. Polymerase Chain Reaction (Polymerase Chain Reaction) PCV2. Porcine Circovirus Type 2 PMWS. Postweaning Multisystemic Wasting Syndrome Taq enzyme Taq DNA polymerase (Taq DNA Polymerase) tm value. Melting temperature (MeltingTemperature)

4 reagent

Unless otherwise specified, only analytical reagents are used. All reagents were treated with RNase-free containers (high pressure after water treatment with DEPC Sterilization) sub-packaging. 4.1 Proteinase K (preparation see Appendix A) - 20 ℃ preservation. 4.2 lysis buffer (preparation see Appendix A) .4 ℃ preservation. 4.3 guanidine isothiocyanate lysis solution (preparation see Appendix A) .4 ℃ preservation. 4.4 Balance phenol (preparation see Appendix A) .4 ℃ preservation. 4.5 phenol - chloroform - isoamyl alcohol (25.24. 1 mixture (preparation see Appendix A) .4 ℃ preservation. 4.6 chloroform - isoamyl alcohol (24. 1) (preparation see Appendix A) .4 ℃ preservation. 4.7 Chloroform. Store at room temperature. 4.8 isopropanol. Store at room temperature. 4.9 70% ethanol Prepared with freshly opened absolute ethanol and sterile deionized water (complying with GB/T 6682), pre-cooled at 20 ° C. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 34745-2017_English be delivered?

Answer: Upon your order, we will start to translate GB/T 34745-2017_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 34745-2017_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 34745-2017_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.