GB/T 34223-2017 English PDF

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GB/T 34223-2017: Determination of the purity of ribonuclease and desoxyribonuclease
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 34223-2017	119	Add to Cart	3 days	Determination of the purity of ribonuclease and desoxyribonuclease	Valid

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Basic data

Standard ID: GB/T 34223-2017 (GB/T34223-2017)
Description (Translated English): Determination of the purity of ribonuclease and desoxyribonuclease
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: A21
Word Count Estimation: 6,628
Date of Issue: 2017-09-07
Date of Implementation: 2018-04-01
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 34223-2017: Determination of the purity of ribonuclease and desoxyribonuclease

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of the purity of ribonuclease and desoxyribonuclease ICS 07.080 A21 National Standards of People's Republic of China Ribonuclease and DNase Purity Detection method 2017-09-07 Posted 2018-04-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard proposed by the China National Institute of Standardization and centralized. This standard was drafted unit. China National Institute of Standardization, Georgia Gelin Biotechnology Co., Ltd., China Biotechnology Development Center, Zhejiang Business University, Agricultural University of Hebei, Hefei University of Technology, Exit Inspection and Quarantine of Hebei Province, Quanzhou Institute of Standardization, Beijing Food Institute of Science, Hebei Food Inspection Institute, Dongying City standardized information, Henan University. The main drafters of this standard. Ma Aijin, Chen Zhiyong, Su Yue, Wang Yanbo, Sun Records, Zheng Lei, Liu Daoliang, Lin Qingshan, Sun Yong, Yun Zhenyu, Fu Linglin, Wu Qi, Zhao Lin, Zhou Wei, Liu Peng, Kang Arts, Zong Xuehua. Ribonuclease and DNase Purity Detection method

1 Scope

This standard specifies the principle of ribonuclease and deoxyribonuclease purity detection, equipment and apparatus, the main reagents, analysis steps And result analysis. This standard applies to biochemical reagents ribonuclease and deoxyribonuclease product production and testing.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods SN/T 3926 exports of milk, eggs, beans Determination of protein content in food Coomassie brilliant blue method

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 RNase ribonuclease; RNase A nuclease that hydrolyzes a phosphodiester bond in ribonucleic acid (RNA) to form an oligonucleotide or a single nucleotide. 3.2 DNase deoxyribonuclease; DNase Phosphodiester hydrolysis in deoxyribonucleic acid (DNA), generating oligonucleotides or mononucleotide nucleases.

4 principle

A certain concentration of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) can separate proteins with different molecular weights, the Coomassie Brilliant Blue (CBB) staining, decolorization imaging, nuclease purity by calculation.

5 equipment and appliances

5.1 electrophoresis system. 5.2 Gel scanning device. 5.3 horizontal decolorization shaker, speed 45r/min. 5.4 Electronic balance. Accuracy of 0.0001g, 0.01g and 0.1g.

6 major reagents

The reagents used in this method are of analytical grade, the experimental water is GB/T 6882 provisions of the secondary water. ......

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