GB/T 33839-2017 English PDF

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GB/T 33839-2017: Methods of transmission electron microscope for biological specimen containing carbon-based nanomaterials involving biological effect
Status: Valid

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Basic data

Standard ID: GB/T 33839-2017 (GB/T33839-2017)
Description (Translated English): Methods of transmission electron microscope for biological specimen containing carbon-based nanomaterials involving biological effect
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: G04
Classification of International Standard: 71.040.40
Word Count Estimation: 11,165
Date of Issue: 2017-05-31
Date of Implementation: 2018-04-01
Quoted Standard: GB/T 18873-2008; GB/T 19619-2004
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the techniques and specifications for the detection of ultrastructures of biological samples containing carbon-based nanomaterials by transmission electron microscopy (hereinafter referred to as transmission electron microscopy). This standard is applicable to the measurement and detection of bio-thin specimens of carbon-based nanomaterials by transmission electron microscopy.

GB/T 33839-2017: Methods of transmission electron microscope for biological specimen containing carbon-based nanomaterials involving biological effect

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Methods of transmission electron microscope for biological imaging containing carbon-based nanomaterials involving biological effect ICS 71.040.40 G04 National Standards of People's Republic of China Based on biological effects of carbon - based nanomaterials Transmission electron microscopy method for biological samples carbon-basednanomaterialsinvolvingbiological effect 2017-05-31 released 2018-04-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Management Committee released Directory Preface I Introduction II 1 Scope 1 2 normative reference document 1 3 Terms and definitions 1 Principle of Method 1 Equipment, reagents and environmental conditions Preparation of bio - thin samples of carbon - based nanomaterials 7 Measurement method 3 Test Report 4 Appendix A (informative) Preparation of glutaraldehyde fixative 5 Appendix B (informative) Preparation of Osmium Oxide Stabilizer 6 Appendix C (informative) epoxy Epon 812 embedding agent preparation 7

Foreword

This standard is drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed by the National Microparraph Analysis Standardization Technical Committee (SAC/TC38). The drafting of this standard unit. the Second People's Medical University of China People's Liberation Army, China Institute of Metrology, National Center for nano-science. The main drafters of this standard. Yang Yongji, Tang Ying, Gao Xitian, Ren Lingling, Wang Xiaoping, Ge Guanglu.

Introduction

It is unclear whether the widely used carbon-based nanomaterials will pose a hazard to biological systems. The main reason there are three. one is the The biological effects of carbon-based nanomaterials on a scale are not clear, including. whether they can enter the organism, how to enter the organism, into the organism Whether it affects the function of the organism; the second is to enter the biological nano-materials testing the lack of adequate technical and test standards; Third, nano-materials There is no standard to be tested for the safety effects of the organism, resulting in the clarity of the safety of the nanomaterials to the organism. Therefore, the development of these three The standard system is an important basis for nanomaterials in life science research. There are many ways to study the biological effects of carbon-based nanomaterials, but the ultrastructures of cells are more intuitive by electron microscopy. Therefore, the study of carbon-based nano-materials on biological tissue cells and even the biological structure of the impact of the current or mainly based on electron microscopy The relevant detection standard is the key to the application of carbon-based nanomaterials in biomedicine. In order to standardize the preparation of carbon-based nanomaterials biological samples Procedures, the correct guidance of transmission electron microscopy detection work, it is necessary to develop this standard. Based on biological effects of carbon - based nanomaterials Transmission electron microscopy method for biological samples

1 Scope

This standard specifies the use of transmission electron microscopy (hereinafter referred to as transmission electron microscopy) detection of carbon-containing nano-materials containing biological samples of the ultrastructure The technology and norms. This standard is applicable to the detection and detection of bio-thin specimens of carbon-based nanomaterials by transmission electron microscopy.

2 normative reference documents

The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article Pieces. For undated references, the latest edition (including all modifications) applies to this document. GB/T 18873-2008 Transmission electron microscopy of biofilm samples - Quantitative analysis of X-ray energy spectrum Glossary of terms for nanomaterials GB/T 19619-2004

3 terms and definitions

GB/T 19619-2004 Definitions and the following terms and definitions apply to this document. 3.1 Carbon-based nanomaterials carbon-basednanomaterials The dispersion phase has at least one carbon material of less than 100 nm in one dimension. Note. The dispersed phase consists of carbon atoms. Carbon-based nanomaterials mainly include three types, one-dimensional carbon-based nanomaterials (including carbon nanotubes and carbon nanofibers Etc.), two-dimensional carbon-based nanomaterials (including graphene and nano-graphite sheets, etc.) and zero-dimensional carbon-based nanomaterials (including carbon nanoparticles and fullerene balls Wait). 3.2 Biological effect biologicaleffect The interaction between nanomaterials and life processes leads to changes in the morphological structure and function of organisms. 3.3 Biological thin sample biologicalthinspecimen A thin sample of 40 nm ~ 100 nm was used for microfilm slices for transmission electron microscopy.

4 Principle of the method

The surface physical and chemical properties of the carbon-based nanomaterials make them easy to form agglomerate, which is dispersed and contacted with the organism. Carbon - based nanomaterials and organisms After exposure, the use of ultra-micro-structure sample preparation method, made of biological thin sample. The carbon nanomaterials were detected by transmission electron microscopy The ultrastructural changes of cells and organelles produced to determine the biological effects of carbon-based nanomaterials on organisms. With high energy electron beam irradiation Biological thin sample of the tiny area, the incident electron beam through the thin sample, the transmission electrons carry a biological thin sample internal information, imaging The ultrastructural image of the bio-thin specimen is formed. ......

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