GB/T 29581-2013 English PDF

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GB/T 29581-2013: Detection and identification of Xanthomonas axonopodis pv. betlicola (Patel et al.) Vauterin et al.
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 29581-2013	279	Add to Cart	3 days	Detection and identification of Xanthomonas axonopodis pv. betlicola (Patel et al.) Vauterin et al.	Valid

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Basic data

Standard ID: GB/T 29581-2013 (GB/T29581-2013)
Description (Translated English): Detection and identification of Xanthomonas axonopodis pv. betlicola (Patel et al.) Vauterin et al.
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 12,151
Quoted Standard: GB/T 4789.28-2003
Regulation (derived from): National Standards Bulletin 2013 No. 10
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies: pepper leaf spot fungus (Xanthomonas axonopodis pv. betlicola) quarantine and identification methods. This standard applies to plant seedlings and other propagation materials and tissues pepper leaf spot fungus quarantine and iden

GB/T 29581-2013: Detection and identification of Xanthomonas axonopodis pv. betlicola (Patel et al.) Vauterin et al.

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Xanthomonas axonopodis pv. Betlicola (Patel et al.) Vauterin et al. ICS 65.020.01 B16 National Standards of People's Republic of China Pepper quarantine and identification of bacterial leaf spot (Pateletal.) Vauterinetal. Issued on. 2013-07-19 2013-12-06 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China Hainan Entry-Exit Inspection and Quarantine Bureau, Shenzhen People's Republic of China Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Guangdong Entry Inspection and Quarantine Bureau. The main drafters of this standard. Liu Fuxiu, Feng Lixia, Longhai, Li Weidong, apricot Ling, Han Yuchun, super first week. Pepper quarantine and identification of bacterial leaf spot

1 Scope

This standard specifies the pepper leaf spot fungus (Xanthomonasaxonopodispv.betlicola) quarantine and identification methods. This standard applies to plant seedlings and other propagation quarantine and identification of materials and tissues pepper leaf spot fungus.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 4789.28-2003 Microbiological examination of food hygiene staining, media and reagents

3 pepper lunata basic information

Chinese name. pepper leaf spot fungus (bacterial leaf spot of pepper; carpet grass Xanthomonas betel pv). Scientific name. Xanthomonasaxonopodispv.betlicola (Patel, Kulkarni Kersters Synonyms. Xanthomonascampestrispv.betlicola (Patel, Kulkarni Disease English name. bacterialleafspotofpepper, pepperbacterialleafspot. Genus Prokaryotae Procaryotes, proteobacteria door Proteobacteria, γ- Proteobacteria Gang Gammaproteobacteria, Xanthomonas Aeromonas head Xanthomonadales, Xanthomonas Branch Xanthomonadaceae, Xanthomonas Xanthomonas, Xanthomonas carpet grass pole Bacteria Xanthomonasaxonopodis. For additional information, see Appendix A. pepper leaf spot fungus

4 principle of the method

According to host range pepper leaf spot fungus, cultural, physiological and biochemical characteristics and the characteristics of the symptoms caused by the disease, the use of pathogens stars Off, the bacteria culture method traits observation, physiological and biochemical characteristics testing and pathogenicity determination and other detection and identification.

5 Reagents and culture medium

5.1 Reagents Sterile water, agar, yeast extract, peptone, glucose, nutrient agar, beef extract, sodium chloride, sodium hydroxide. Fructose, xylose, galactose, lactose, maltose, mannitol, trehalose, glycerol, salicin, sucrose, sorbose, rhamnose, raffinose, muscle Alcohol, sodium oxalate, β- hydroxybutyrate sodium succinate, malate, sodium lactate, sodium citrate, sodium tartrate, DL- arginine gelatine, litmus milk. 5.2 Medium Bacteria isolated using NGA medium (see Appendix B), the proliferation of bacteria using nutrient agar (see Appendix B). ......

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