GB/T 29431-2012 English PDF

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GB/T 29431-2012: Detection and identification of Clavibacter michiganensis subsp. michiganensis (Smith) Davis et al.
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 29431-2012	279	Add to Cart	3 days	Detection and identification of Clavibacter michiganensis subsp. michiganensis (Smith) Davis et al.	Valid

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Basic data

Standard ID: GB/T 29431-2012 (GB/T29431-2012)
Description (Translated English): Detection and identification of Clavibacter michiganensis subsp. michiganensis (Smith) Davis et al.
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 12,135
Quoted Standard: SN/T 2122
Regulation (derived from): National Standards Bulletin 2012 No. 42
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the tomato canker quarantine identification. This standard applies to tomato seeds, seedlings and other related materials Solanaceae tomato canker quarantine and identification.

GB/T 29431-2012: Detection and identification of Clavibacter michiganensis subsp. michiganensis (Smith) Davis et al.

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Clavibacter michiganensis subsp.michiganensis (Smith) Davis et al. ICS 65.020.01 B16 National Standards of People's Republic of China Tomato canker quarantine and identification methods michiganensis (Smith) Davisetal. Issued on. 2012-12-31 2013-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China Zhuhai Entry-Exit Inspection and Quarantine Bureau, South China Agricultural University, China Agricultural University, Chinese people Republic of Beijing Exit Inspection and Quarantine. The main drafters of this standard. Le ocean, Peng Ren, Liu Qiongguang, Luo Xin, Zhang Jianjun, Liu Yong, Feng Yan, Zhong Gong years, Lin Youwei, Zhang Weidong, Gao Na. Tomato canker quarantine and identification methods

1 Scope

This standard specifies the tomato canker quarantine authentication methods. This standard applies to tomato seeds, seedlings and other material related Solanaceae tomato canker quarantine and identification.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. SN/T 2122 entry and exit quarantine of plants and plant products sampled

3 tomato canker basic information

Chinese name. Tomato canker (rod-shaped bacilli Michigan Michigan subspecies). Scientific name. Clavibactermichiganensissubsp.michiganensis (Smith, 1910) Davisetal, 1984, referred to. Cmm). Synonyms. CorynebacteriummichiganensepV.michiganense (Smith) Dye michiganense (Smith) Jensen. Disease English. Bacterialcankeroftomato. Spp domain Bacteria, Actinobacteria Actinobacteria, actinomycetes Actinobacteria, Actinomycetales Actinomvcetales, Microbacterium Branch Microbacteriaceae, Rod genus Clavibacter. Long-distance spread of the pathogen mainly by contaminated seed, tomato seed inner and outer layers can be contaminated. In the field and greenhouse, the bacteria from injury Mouth, porosity and natural orifice invade host tissue, mainly through irrigation water, rain water, pruning shears, training materials and other communication. The bacteria in the soil and disease Debris organization can survive for 2 years to 3 years. Other basic information, see Appendix A. The tomato canker

4 principle of the method

According hazards like tomato canker, the disease symptoms of suspected disease quarantine site or field survey found that samples were collected and Back to the lab for further isolation and identification, according to the culture traits pathogens, pathogenic response and molecular characterization of the species were determination.

5 apparatus and appliances

Biological microscope, clean benches, Autoclave, light incubator, temperature oscillation incubator, electronic scales, deep freezer, then Three Ring, toothpicks, syringes, heated water bath, high-speed refrigerated centrifuge (12000r/min or more), a homogenizer, PCR instrument, electrophoresis apparatus (electric Swimming instrument and horizontal electrophoresis tank), gel imaging analyzer, micro adjustable pipette and the like. ......

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