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GB/T 28975-2012 English PDF

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GB/T 28975-2012: Detection and identification of lettuce mosaic virus -- Nanoparticles-enlarged in colloidal gold immunochromatographic assay
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GB/T 28975-2012229 Add to Cart 3 days Detection and identification of lettuce mosaic virus -- Nanoparticles-enlarged in colloidal gold immunochromatographic assay Valid

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Basic data

Standard ID: GB/T 28975-2012 (GB/T28975-2012)
Description (Translated English): Detection and identification of lettuce mosaic virus -- Nanoparticles-enlarged in colloidal gold immunochromatographic assay
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 10,178
Quoted Standard: GB 19489; GB/T 23629-2009; GB/T 23632-2009
Regulation (derived from): National Standards Bulletin No. 41 of 2012
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the use of nanoparticles sensitized colloidal gold immunochromatographic assay lettuce mosaic virus theory, experimental procedures and results of determination and so on. This standard applies to the organization and its products

GB/T 28975-2012: Detection and identification of lettuce mosaic virus -- Nanoparticles-enlarged in colloidal gold immunochromatographic assay


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of lettuce mosaic virus. Nanoparticles-enlarged in colloidal gold immunochromatographic assay ICS 65.020.01 B16 National Standards of People's Republic of China Lettuce Mosaic Virus quarantine and identification of nanoparticles Sensitized colloidal gold immune chromatography Issued on. 2012-12-31 2013-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Hunan Exit Inspection and Quarantine. The main drafters of this standard. Li Jinfeng, Chen Yan, Maji Xiang, Tian Shimin, Dai Hua, Zhang Fan, Jin Yong, Zou Mingjiang.

Introduction

When issuing authority of the document drew attention to declare compliance with this document may involve 6.4.3, B.4 and sensitizing agent-related patents usage of. The issuing authority for the patent document authenticity, validity and scope without any position. The patent holder has to ensure that the issuing authority of the document, he was willing to reasonable and non-discriminatory terms and conditions with any applicant, Patent licensing negotiations. The patent holder's statement has been filed in the release mechanism of the present document. Related information is available through the following Contact at. The patent holder. Chinese Academy of Inspection and Quarantine Contact. Zou Mingjiang Address. Gaobeidian Road, Chaoyang District, Beijing, No. 3 Chinese Academy of Inspection and Quarantine Zip code. 100123 Please note that in addition to the above patents, some of the contents of this document can still be involved in the patent. The issuing authority of this document does not bear this recognition These patent liability. Lettuce Mosaic Virus quarantine and identification of nanoparticles Sensitized colloidal gold immune chromatography

1 Scope

This standard specifies the use of nanoparticles sensitizing GICA assay lettuce mosaic virus in principle, experimental procedures and results of judgment Set and so on. This standard applies to plants and their products in the rapid screening of tissue lettuce mosaic virus.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB 19489 General requirements for laboratory biosafety GB/T 23629-2009 introduction of plant pathogen control technology requirements for safety GB/T 23632-2009 intercepted entry plant quarantine harmful biological appraisal review procedures

3 Basic information of lettuce mosaic virus

Chinese name. lettuce mosaic virus Name. lettucemosaicvirus Abbreviation. LMV Taxonomic position. potato virus Y Branch (Potyviridae) genus of potato virus Y (Potyvirus). For additional information Lettuce Mosaic Virus in Appendix A.

4 principle of the method

Based on the principle of colloidal gold immunochromatography double antibody sandwich assay using chloroauric acid and a reducing agent produced gold nanoparticles to conventional GICA strip processing, improve the detection sensitivity, according to changes in the color of the test strip, the sample is determined whether or not there Lettuce mosaic virus. 5 equipment, appliances, materials and reagents 5.1 Equipment Balance (a sense of volume, 1/10000g), centrifuges, organization stamp mill, low-temperature refrigerator. 5.2 Appliances Micropipette (2.5μL, 10μL, 20μL, 100μL, 200μL, 1000μL), mortar tubes and the like.
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