GB/T 28630.1-2012 English PDF

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GB/T 28630.1-2012: Diagnostic protocols for white spot disease -- Part 1: Dot blot hybridization method with DNA probe
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 28630.1-2012	299	Add to Cart	3 days	Diagnostic protocols for white spot disease -- Part 1: Dot blot hybridization method with DNA probe	Valid

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GB/T 25165   NY/T 1663   GB/T 20365   GB/T 28630.4   GB/T 28630.5   GB/T 28630.3   

Basic data

Standard ID: GB/T 28630.1-2012 (GB/T28630.1-2012)
Description (Translated English): Diagnostic protocols for white spot disease -- Part 1: Dot blot hybridization method with DNA probe
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 65.020.30
Word Count Estimation: 13,144
Quoted Standard: GB/T 28630.4-2012
Regulation (derived from): National Standards Bulletin 2012 No. 17
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the spot syndrome nucleic acid probe dot blot method requires reagents and materials, equipment, procedures, and results of determination. This section applies to shrimp as shrimp, shrimp, larvae, larvae and eggs of live, chilled o

GB/T 28630.1-2012: Diagnostic protocols for white spot disease -- Part 1: Dot blot hybridization method with DNA probe

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS65.020.30 B41 National Standards of People's Republic of China (WSD) diagnostic protocols spot syndrome Part 1. nucleic acid probe dot blot hybridization assay Part 1. DotblothybridizationmethodwithDNAprobe Issued on. 2012-11-01 2012-07-31 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

GB/T 28630 "white spot syndrome (WSD) diagnostic protocols" is divided into five parts. --- Part 1. nucleic acid probe dot blot hybridization assay; --- Part 2. nested PCR assay; --- Part 3. In situ hybridization assay; --- Part 4. Histopathological diagnosis method; --- Part 5. TE fresh tissue staining. This is Part 1 GB/T 28630 in. Rules in this section in accordance with GB/T 1.1-2009 given draft. This section proposed by the People's Republic of China Ministry of Agriculture. This part of the National Standardization Technical Committee on Fisheries (SAC/TC156) centralized. This section drafted by. Chinese Academy of Fishery Sciences Yellow Sea Fisheries Research Institute, Ministry of Agriculture, the National Fisheries Extension Center. The main drafters of this section. Huang Jie, Yang Bing, Chen Aiping, Song Xiaoling, the history of the silver, Yang Cong Hai, Wei Qi, Liu Li.

Introduction

When issuing authority of the document drew attention to the statement of compliance with this document, related to the national invention patent "shrimp epidemic pathogen detection reagent Kit and detection method "(ZL00111336.4) use. The issuing authority for the patent document authenticity, validity and scope without any position. The patent holder has to ensure that the issuing authority of this document, they are willing to work with any applicant reasonable and non-discriminatory terms and conditions Under patent license negotiations. The patent holder's statement has been filed in the release mechanism of the present document. Related information can be Contact the following ways. Patent Holder Name. History of the silver, Yang Bing, Huang Jie, Song Xiaoling Yang Cong sea. Address. Chinese Academy of Fishery Sciences Yellow Sea Fisheries Research Institute, Nanjing Road, Qingdao City, Shandong Province, No. 106. Please note that in addition to the above patents, some of the contents of this document may still involve patents issuing authority of this document does not undertake to recognize these special Lee responsibility. (WSD) diagnostic protocols spot syndrome Part 1. nucleic acid probe dot blot hybridization assay

1 Scope

This section GB/T 28630 provisions of the White Spot Syndrome DNA probe dot blot hybridization methods needed reagents and materials, equipment, Procedure and result judgment. This section applies to shrimp as shrimp, young shrimp, shrimp larvae, juveniles and fertilized in vivo, chilled or frozen products and other white spot syndrome Virus sensitive crustaceans White Spot Syndrome pathogen screening, clinical diagnosis of the condition, the estimate does not apply to active viral infection.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated edition applies to this article Member. Where not dated references, the latest edition (including any amendments) applies to this document. Diagnostic protocols GB/T 28630.4-2012 spot syndrome (WSD) Part 4. Histopathological diagnosis method Principle 3 Pathogens and pathological features of White Spot Syndrome 3.1 See GB/T 28630.4-2012 2.1. 3.2 Technical Principle In non-radioactive marker --- digoxigenin labeled White Spot Syndrome Virus (WSSV) DNA fragment syndrome, or nucleic acid probes, and present in the Samples of viral DNA specificity of nucleic acid hybridization, by an antigen - antibody reaction and enzyme - substrate chemical color reaction like display Products in the presence of a virus.

4 Reagents and materials

4.1 Unless otherwise stated in the analysis using only recognized as analytical grade reagents and distilled or deionized water or equivalent purity. 4.2 chlorine disinfectant. medical supplies or aquatic drugs. 4.3 Hybrid Membrane. nitrocellulose membrane. 4.4 PCRDIGProbeSynthesisKit1). 4.5 Sodium lauryl sarcosine (Sarkosyl). chemical reagents. 4.6 sodium dodecyl sulfate (SDS). chemical reagents. 4.7 disodium edetate (EDTA-Na2 · 2H2O). chemical reagents. 4.8 mercaptoethanol. biochemical reagents. 4.9 Tris balance phenol (saturated phenol). biochemical reagents. 1) PCRDIGProbeSynthesisKit is provided by Roche, the trade name of the product. This information is given in order to facilitate the use of this section Who does not imply endorsement of the product. If other equivalent product has the same effect, you can use these equivalent products. ......

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