GB/T 28063-2011 English PDF
Basic dataStandard ID: GB/T 28063-2011 (GB/T28063-2011)Description (Translated English): Detection and identification of Bean pod mottle virus Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B16 Classification of International Standard: 65.020.01 Word Count Estimation: 14,153 Date of Issue: 2011-12-30 Date of Implementation: 2012-06-01 Regulation (derived from): Announcement of Newly Approved National Standards No. 23 of 2011 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the bean pod mottle virus serology and molecular biology procedures and methods of detection and identification. This standard applies may carry the virus legume seeds, seedlings and other plant propagating material detection and identification, but also to spread the virus detection and identification of insects mediator. GB/T 28063-2011: Detection and identification of Bean pod mottle virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Detection and identification of Bean pod mottle virus ICS 65.020.01 B16 National Standards of People's Republic of China Bean pod mottle virus, quarantine and identification methods Issued on. 2011-12-30 2012-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China, Xiamen Entry-Exit Inspection and Quarantine Fujian People's Republic of China, People's Republic of China, Shenzhen CIQ. The main drafters of this standard. Liao Furong, Shen Jianguo, Zheng Yun, Chen Qing, Lin Shiming, Chen Yun, Huang Peng Ying, Wu Yuan. Bean pod mottle virus, quarantine and identification methods1 ScopeThis standard specifies the method for detection and identification of bean pod mottle virus serology and molecular biology. This standard applies may carry the virus detection and identification of legume seeds, seedlings and other plant propagation material, but also to spread The mediator virus detection and identification of insects.2 Basic information of bean pod mottle virusChinese name. bean pod mottle virus. English name. beanpodmottlevirus. Synonyms. beanpodmottlecomovirus (bean pod mottle virus), podmottlevirus (pod mottle virus), desmodium virus (loosestrife virus). English abbreviation. BPMV. Genus cowpea mosaic virus family Comoviridae, cowpea mosaic virus genus Comovirus. Bean clip Mottle Virus For additional information, see Appendix A.3 PRINCIPLE OF THE METHODThe use of double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) based on antigen-antibody reaction, reverse transcription and in vitro DNA amplification technology Surgery reverse transcription polymerase chain reaction (RT-PCR) detection and identification.4 Main equipmentThe standard detection methods identified mainly with the following equipment. Trace juicer, microplate reader, washer, microbalance (sense of volume. 0.001g), PCR, fluorescence PCR, electrophoresis, horizontal electrophoresis Groove, gel imager, desktop high-speed refrigerated centrifuge, water bath, pH meter range and a variety of adjustable pipette (1000μL, 200μL, 100μL, 20μL, 2μL). Preparation of the test sample 5 Preparation of 5.1 DAS-ELISA and IC-RT-PCR for detecting samples Inspection of each batch of samples symptom checker, prefers the seeds have brown or black mottle symptoms (from the hilum start), or choose Having a blade symptoms mottled, wrinkled, chlorosis and so on. Seed samples. Weigh 0.5g ~ 1.0g seed coat as the test sample, is thoroughly ground in liquid nitrogen and warmed at 1.10 after added Sample extraction buffer and grinding continued. Leaf samples. Weigh 0.5g ~ 1.0g leaves as a test sample, grind in a mortar or grinding in liquid nitrogen, 1.10 Sample extraction buffer was added and grinding continued. The ground sample was transferred to 5mL centrifuge tube, 8000r/min centrifugal 5min, supernatant as DAS-ELISA (see Annex Record B) and IC-RT-PCR (see Appendix C) detection extract. Note. The extract used in 3h, otherwise the stored at 4 ℃. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 28063-2011_English be delivered?Answer: Upon your order, we will start to translate GB/T 28063-2011_English as soon as possible, and keep you informed of the progress. The lead time is typically in 9 seconds (download/delivered in 9 seconds). The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 28063-2011_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 28063-2011_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay. |