GB/T 27819-2011 English PDFUS$139.00 ยท In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 27819-2011: Chemicals -- Test method of in vitro mammalian cell transformation Status: Valid
Basic dataStandard ID: GB/T 27819-2011 (GB/T27819-2011)Description (Translated English): Chemicals -- Test method of in vitro mammalian cell transformation Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: A80 Classification of International Standard: 13.300; 11.100 Word Count Estimation: 6,646 Date of Issue: 2011-12-30 Date of Implementation: 2012-08-01 Regulation (derived from): Announcement of Newly Approved National Standards No. 23 of 2011 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the chemical transformation of mammalian cells in vitro test principles, test methods, test data and reports. This standard can be used as the carcinogenic effects of chemicals alternative detection methods. GB/T 27819-2011: Chemicals -- Test method of in vitro mammalian cell transformation---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Chemicals. Test method of in vitro mammalian cell transformation ICS 13.300; 11.100 A80 National Standards of People's Republic of China Chemicals in vitro mammalian cell transformation test methods Issued on. 2011-12-30 2012-08-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard and the European Commission Regulation 440/2008. the European Parliament and the Council on the Registration, Evaluation, Authorisation and Restriction Method B.21 (REACH) No. 1907/2006 Test method regulations (2008) "in vitro mammalian cell transformation test Test "is consistent (in English) of technical content. The standard structure and made the following editorial changes. --- Increasing the range a chapter; --- Change the unit of measure of legal units of measurement. This standard is managed by the National Standardization Technical Committee chemicals dangerous (SAC/TC251) and focal points. This standard was drafted. Chinese Center for Disease Prevention and Control of Occupational Health and Poison Control, Centers for Disease Control and Prevention in Beijing, China Chemical Technology Development Center. The main drafters of this standard. Muslim group effect, Chao-lin, Wang Xiaobing, Xiong Xiaoyan, Zhao Sujuan, Wei Wu Ai. Chemicals in vitro mammalian cell transformation test methods1 ScopeThis standard specifies the principles of testing chemicals in vitro mammalian cell transformation test, test methods, test data and reports. This standard can be used as an alternative method for detecting the carcinogenic effects of chemicals. Test Principle 2 Mammalian cell culture test system can be used to detect phenotypic changes caused by chemicals, such a change in their body system malignant Transformation related. Widely used cell lines include C3H10T1/2,3T3, SHE, Fischer rat cells, the morphological observation of cells, Transformed foci formation or anchorage independence changes in soft agar. The system is also used for some other students to detect cells after exposure to chemicals Management and morphological changes, but not widely. There is currently no in vitro test for detection of the end proved to have a clear relationship with cancer mechanisms. Some detection system can be detected cancer-promoting agent. Test substance for colony formation (colony formation rate) by measuring or cultured cell growth rate of May reflect the influence of the size of its cytotoxicity, it can be calculated by exposure and toxicity associated cytotoxicity evaluation, but because of some trials We need to extend or replace the dish incubation time, therefore, sometimes cytotoxicity data can not be used to calculate the conversion rate. Test Method 3 3.1 Test preparation 3.1.1 Cell Many cell lines and primary cells can be used as a test cell, select the appropriate cell type transformation test according to the method chosen. Before the test, the researchers should ensure that the test cell will produce the corresponding phenotypic change after exposure to carcinogens, the laboratory should have the record show that this table Type change is effective and reliable. 3.1.2 medium (liquid) The culture medium and experimental conditions should be suitable for cell transformation test. 3.1.3 test substance Before exposure cell, the test substance to be mixed with the culture medium, a suitable solvent may also be first dissolved or made into a suspension. Medium solution Final concentration of agent should not affect cell viability, growth rate or conversion rate. 3.1.4 metabolic activation Cells should have, respectively, under appropriate conditions without the presence of metabolic activation system exposed; when the type used in the cells themselves have the ability to metabolic activation When, you should consider whether it is suitable activation characteristics and chemical properties of the test substance. 3.2 Test conditions 3.2.1 Negative and positive controls Each test shall be a direct effect of each selected compound and a need metabolic activation of a compound as a positive control. another ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 27819-2011_English be delivered?Answer: Upon your order, we will start to translate GB/T 27819-2011_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. 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