GB/T 27521-2011 English PDF

US$229.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 27521-2011: RT-PCR assay for swine influenza virus nucleic acid
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 27521-2011	229	Add to Cart	3 days	RT-PCR assay for swine influenza virus nucleic acid	Valid

Similar standards

GB/T 25165   NY/T 1663   GB/T 20365   GB/T 27530   GB/T 27517   GB/T 27531   

Basic data

Standard ID: GB/T 27521-2011 (GB/T27521-2011)
Description (Translated English): RT-PCR assay for swine influenza virus nucleic acid
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 10,192
Date of Issue: 2011-11-21
Date of Implementation: 2012-03-01
Regulation (derived from): Announcement of Newly Approved National Standards No. 18 of 2011
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the swine influenza virus nucleic acid by RT-PCR method for the technical requirements. This standard specifies the RT-PCR detection method for the detection of porcine lung tissue, nasal and tracheal secretions and cultures of these samples were collected in the swine influenza virus nucleic acid.

GB/T 27521-2011: RT-PCR assay for swine influenza virus nucleic acid

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
RT-PCR assay for swine influenza virus nucleic acid ICS 11.220 B41 National Standards of People's Republic of China Swine influenza virus nucleic acid by RT-PCR method Issued on. 2011-11-21 2012-03-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. The standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Standardization Technical Committee Animal Epidemic Prevention (SAC/TC181) centralized. This standard was drafted. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Chinese Academy of Inspection and Quarantine, China Institute of Veterinary Drug monitoring The police. The main drafters of this standard. Qiaozhuan Ling, Han Xueqing, Yanghuan Liang, Liu Ye Bing, Chen Yan, Wu Shaojiang, Chen Hualan, Lin Xiangmei, Xin Xiaoguang, Zhuzhong Wu, Wang Huiyu, Liu, Li, Mei Lin, Wang Yiqin, Xu Biao, Ruanzhou Xi, Yi Ning Po, such as thin clear, Qin Zhifeng, Lin Zhixiong. Swine influenza virus nucleic acid by RT-PCR method

1 Scope

This standard specifies the technical requirements for swine influenza virus nucleic acid by RT-PCR methods. RT-PCR detection method specified in this standard for the detection of swine lung tissue, nasal and tracheal secretions and culture of these samples were collected It was the swine flu virus nucleic acid.

2 Acronyms

The following abbreviations apply to this document. DEPC. diethyl pyrocarbonate (diethypyrocarbonate) dNTP. deoxynucleotide triphosphate (deoxy-ribonucleosidetriphosphate) EB. ethidium bromide (ethidiumbromide) M-MLVRT. Moloney murine leukemia virus reverse transcriptase (moloneymurineleukemiavirusreversetran- scriptase) PCR. polymerase chain reaction (polymerasechainreaction) RNA. ribonucleic acid (ribonucleicacid) RNAaseinhibitor. RNA inhibitors RT-PCR. reverse transcription - polymerase chain reaction (reverse-transcriptionpolymerasechainreaction) SPF. specific pathogen free (specificpathogenfree) TaqDNApolymerase. TaqDNA polymerase

3 Instrument

3.1 PCR instrument. 3.2 low-speed desktop centrifuge. 3.3 electrophoresis. 3.4 electrophoresis tank. 3.5 refrigerator. 3.6 gel imaging system. 3.7 micropipette. 3.8 water bath.

4 Reagents

4.1 LSTRIzolRNA RNA extraction reagents or other commercially available extraction reagent. 4.2 of chloroform, isopropanol, ethanol. 4.3 1.2% agarose gel, A in Appendix A.1. 4.4 50 × TAE buffer, see Appendix A A.2. ......

Image     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 27521-2011_English be delivered?

Answer: Upon your order, we will start to translate GB/T 27521-2011_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 27521-2011_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 27521-2011_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.