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GB/T 25168-2010 English PDF

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US$139.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 25168-2010: Technical regulation of farm animals cDNA library construction and preservation
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 25168-2010	139	Add to Cart	3 days	Technical regulation of farm animals cDNA library construction and preservation	Valid

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Basic data

Standard ID: GB/T 25168-2010 (GB/T25168-2010)
Description (Translated English): Technical regulation of farm animals cDNA library construction and preservation
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B40
Classification of International Standard: 65.020.30
Word Count Estimation: 6,687
Date of Issue: 2010-09-26
Date of Implementation: 2011-03-01
Quoted Standard: GB/T 6682
Regulation (derived from): National Standard Approval Announcement 2010 No.6 (Total No.161)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the animal cDNA library construction methods. This standard applies to pigs, cattle, sheep, horses, asses, chicken, ducks, geese and other poultry cDNA library construction and preservation.

GB/T 25168-2010: Technical regulation of farm animals cDNA library construction and preservation

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Technical regulation of farm animals cDNA library construction and preservation ICS 65.020.30 B40 National Standards of People's Republic of China Livestock cDNA library construction and preservation of technical regulations Issued on. 2010-09-26 2011-03-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

The standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Standardization Technical Committee on Animal Husbandry (SAC/TC274) centralized. This standard was drafted. Beijing Animal Husbandry and Veterinary Research Institute, Chinese Academy of Agricultural Sciences. The main drafters of this standard. Mayue Hui, Wei Guan Jun, Lu Taofeng, Lixiang Chen, Tong Chun-ling, Lulu Yu, Liu Peng. Livestock cDNA library construction and preservation of technical regulations

1 Scope

This standard specifies the livestock cDNA library construction methods. This standard applies to pigs, cattle, sheep, horses, donkeys, chickens, ducks, geese and other poultry cDNA library construction and preservation.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. Laboratory use specifications and test methods GB/T 6682 Analysis

3 Terms and Definitions

The following terms and definitions apply to this standard. 3.1 cDNA complementarydeoxyribonucleicacid Mature mRNA as a template, under the action of reverse transcriptase synthesis of cDNA strand. 3.2 cDNA library cDNAlibrary Total RNA will be in a particular organ or tissue specific developmental stage eukaryotic organisms extracted, through a series of biochemical reverse transcription, etc. After the double-stranded cDNA was synthesized group, and then inserted into a suitable vector, the transformed host strains constitute a specific cDNA clone groups. 3.3 Oligonucleotide oligonucleotide A class of short chain of nucleotides or less collectively only 20 base pairs, commonly used as a probe to determine the structure of DNA or RNA. 3.4 Plaque plaque Was released phage infection of bacteria progeny phage, we will continue to infect surrounding cells, resulting in the growth of bacteria on agar plates moss There are lysed, the transparent region is formed of a large number of cells.

4 Reagents and solution preparation

Unless otherwise specified, reagents were analytical grade or biochemical reagents. Test water meet the requirements of GB/T 6682 a water use. 4.1 diethylpyrocarbonate (diethypyrocarbonate, DEPC) treated water In 1000mL of deionized water was added 1mLDEPC, 37 ℃ allowed to stand 12h, autoclaved (120 ℃ 30min). 4.2 2mol/LMgSO4 Weigh 246.50gMgSO4 · H2O, add 400mL double-distilled water (doubledistiledH2O, ddH2O) dissolved, set the volume to 500mL, autoclaving. 4.3 10mmol/LMgSO4 Measure 1mL2mol/LMgSO4 plus ddH2O constant volume to 200mL, autoclave, 30min. 4.4 1mol/LTris-Cl (pH7.5) Weigh 121.14gTris, 800mLddH2O dissolved in alkali to adjust pH to 7.5, set the volume to 1L, autoclaved. 4.5 SM buffer Weigh 5.80gNaCl, 2.00gMgSO4 · 7H2O, weighed 50mL1mol/LTris-Cl (pH7.5) and 5mL2% (mass ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 25168-2010_English be delivered?

Answer: Upon your order, we will start to translate GB/T 25168-2010_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 25168-2010_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 25168-2010_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.