GB/T 24829-2009 English PDF

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GB/T 24829-2009: Identification of Trichodorus species as virus vectors for import and export quarantine
Status: Valid

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Basic data

Standard ID: GB/T 24829-2009 (GB/T24829-2009)
Description (Translated English): Identification of Trichodorus species as virus vectors for import and export quarantine
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 30,326
Date of Issue: 2009-12-15
Date of Implementation: 2010-06-01
Quoted Standard: SN/T 1157; SN/T 1158
Regulation (derived from): National Standard Approval Announcement 2009 No.15 (Total No.155)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the nematode genus burr pass quarantine and identification of toxic species. This standard applies to plants, media and soil nematode genus burr pass quarantine and identification of toxic species.

GB/T 24829-2009: Identification of Trichodorus species as virus vectors for import and export quarantine

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Identification of Trichodorus species as virus vectors for import and export quarantine ICS 65.020.01 B16 National Standards of People's Republic of China Burr nematode genera (mass poisoning species) identification of quarantine Posted 2009-12-15 2010-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Table of Contents

Preface Ⅰ 1 Scope 1 2 Normative references 1 3 Identification 1 basis Apparatus and appliances 4 1 5 Reagent 1 6 2 quarantine site 7 Laboratory test 2 8 morphological identification of 2 Analyzing Results 9 4 10 4 Sample Storage Appendix A (informative) virus transmission glitch nematode species basic information table 5 Annex B (informative) nematode separation method 6 Annex C (informative) nematode slide specimen preparation method 7 Annex D (informative) four kinds of virus transmission glitch nematode dynamometers value Table 8 Appendix E (normative) Identification of the genus burr polytomous keys (male) 9 Annex F (informative) Identification of the genus burr polytomous keys (female) 13 Annex G (informative) multi-manifold retrieval application reference table 17 Appendix H (informative) four kinds of mass poisoning kinds of patterns 22 References 26

Foreword

The Standard Appendix A, Appendix B, Appendix C, Appendix D, Appendix E, Appendix F, Annex G and Annex H are informative appendices. This standard by the National Standardization Technical Committee on plant quarantine and centralized. This standard was drafted. People's Republic of China, Ningbo Entry-Exit Inspection and Quarantine Bureau Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, CIQ Yunnan, Zhejiang University. The main drafters of this standard. Gu Jianfeng, Zhao Lirong, Chen Xianfeng, Zheng Wei, Li Xu Qing, Du Yu. Burr nematode genera (mass poisoning species) identification of quarantine

1 Scope

This standard specifies the case of virus transmission glitch nematode species of quarantine and identification methods. This standard applies to plants, and soil media glitch nematode species belong to mass poisoning of quarantine and identification.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. Where dated references, which subsequently Some amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Study whether the latest versions of these documents. Anyone who does not dated reference documents, the latest versions apply to this standard. SN/T 1157 export plant seedlings quarantine rules SN/T 1158 bonsai plant quarantine entry and exit procedures

3 based on identification

Chinese name. Burr nematode genera. English name. stubbyrootnematode. 1877], no side tail gland Gang (AdenophoreaLinstow, 1905), three spear head (TriplonchidaCobb, 1922), membrane Pia mesh (Diph- therophorinaCoomans & Loof, 1970), glitches superfamily [Trichodoridea (Thorne, 1935) Siddiqi, 1974], Section glitch [Trichodoridae (Thorne, 1935) Siddiqi, 1974], burr subfamily (TrichodorinaeThorne, 1935). Burr nematode genus has been found in 56 species of perennial herbaceous or woody roots migratory external parasitic nematodes. Not only directly feeding Plant root cause plant roots chunky, swelling, increased lateral roots and cause the host to malnutrition, growth disorders and other hazards, more serious is known Of which there are four kinds can spread the tobacco rattle virus (tobaccorattlevirus, TRV) and pea early-browning virus (peaearly-browningvi- Glitch nematodes mainly through seedlings with roots, soil and media communication, its female and male is mainly based on morphological characteristics of species identification, host Scope and geographical distribution of information for reference (see Appendix A).

4 apparatus and appliances

4.1 Instrument Biological microscope (50-fold to 1000-fold), stereo microscope (more than 10 times, with the transmission source), electric incubator, pipettes (20μL ~ 200μL), centrifuge (2000r/min or more). 4.2 Appliances Clock face pan or dish, cover slips, slides, scissors, a pick, alcohol lamp, filter paper or gauze nematode, 500μL plastic tubes, cell culture Dish, airtight plastic boxes, funnel, funnel rack, latex tubes, sealing clamp, heating plate, sieve-like (500 mesh).

5 Reagents

4% formaldehyde, glycerol, nail polish, wax, solution Ⅰ (96% ethanol. glycerol. = 20.1.79 distilled water), the solution Ⅱ (% glycerol .96 Ethanol = 1.19). ......

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