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GB/T 23743-2009 English PDF

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GB/T 23743-2009: Microbiological examination of the enumeration of coagulase-positive staphylococci in feeds -- Technique using Baird-Parker agar medium
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GB/T 23743-2009279 Add to Cart 3 days Microbiological examination of the enumeration of coagulase-positive staphylococci in feeds -- Technique using Baird-Parker agar medium Valid

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Basic data

Standard ID: GB/T 23743-2009 (GB/T23743-2009)
Description (Translated English): Microbiological examination of the enumeration of coagulase-positive staphylococci in feeds -- Technique using Baird-Parker agar medium
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B46
Classification of International Standard: 65.120
Word Count Estimation: 12,164
Date of Issue: 2009-05-12
Date of Implementation: 2009-09-01
Adopted Standard: ISO 6888-1-1999/Amd 1-2003, MOD
Regulation (derived from): National Standard Approval Announcement 2009 No.6 (Total No.146)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the coagulase-positive staphylococci in solid medium (Baird-Parker medium), After 36��C �� 1��C under aerobic colony counts cultured technical requirements. This standard applies to feed solidification detect positive staphylococci.

GB/T 23743-2009: Microbiological examination of the enumeration of coagulase-positive staphylococci in feeds -- Technique using Baird-Parker agar medium


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
d) increase in number in the body of the formula. Appendix A of this standard is an informative annex. This standard by the National Feed Industry Standardization Technical Committee (SAC/TC76) and focal points. This standard was drafted. Agricultural Quality Standards and Testing Technology Research Institute, Chinese Academy of Agricultural Sciences [National Feed Quality Supervision and Inspection Center (Beijing)]. The main drafters of this standard. Rao Zhenghua, Lili Bei, Ma Dongxia, Zhang Su, Suxiao Ou. Positive staphylococci feed solidifying microbiology Agar counting method

1 Scope

This standard specifies the coagulase-positive staphylococci in solid medium (Baird-Parker medium), 36 ℃ ± 1 ℃ aerobic conditions After culturing the technical requirements of the colony count. This standard applies to feed clotting enzyme detected positive staphylococci.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 4789.1 Microbiological examination of food hygiene - General GB/T 14699.1 feed sampling (GB/T 14699.1-2005, ISO 6497.2002, IDT) GB/T 20195 preparation of animal feed samples (GB/T 20195-2006, ISO 6498.1998, IDT) Accuracy (trueness and precision) Part 2 ISO 5725-2 test methods and results. Standard Test Method for determining the repeatability And basic methods of reproduction ISO 16140 Microbiology of food and animal feed draft validation of alternative methods

3 Terms and Definitions

The following terms and definitions apply to this standard. 3.1 Under test conditions specified in this standard, cultured in selective solid medium can be formed of typical and/or atypical colonies and plasma coagulation Enzyme test positive for a group of bacteria. 3.2 According to the method specified in this standard, per milliliter or per gram of sample coagulated number of positive staphylococci. Principle 4 4.1 quantitatively plated on solid selective medium by surface inoculation, inoculation two plates. Direct inoculation liquid sample, its He required 1.10 dilution was inoculated samples. In other dilution, dilution was inoculated with the above method. Two plates inoculated with each dilution. 4.2 Petri dishes at 36 ℃ ± 1 ℃ aerobic culture, the culture 24h and 48h time have to be checked. 4.3 Select the appropriate dilution level, the number of colonies grown dish, per gram (per milliliter) sample contains coagulase-positive grapes The number of bacteria.

5 Diluent and culture medium

5.1 General Unless otherwise indicated in the analysis using only recognized as analytical reagents, laboratory water using distilled or deionized water or substantially pure
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