GB/T 23195-2008 English PDFUS$149.00 · In stock
Delivery: <= 2 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 23195-2008: Method for the determination of catalase in bee pollen -- Ultraviolet spectrophotometry Status: Valid
Basic dataStandard ID: GB/T 23195-2008 (GB/T23195-2008)Description (Translated English): Method for the determination of catalase in bee pollen -- Ultraviolet spectrophotometry Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B47 Classification of International Standard: 65.140 Word Count Estimation: 5,524 Date of Issue: 2008-12-31 Date of Implementation: 2009-06-01 Quoted Standard: GB/T 6682 Regulation (derived from): National Standard Approval Announcement 2008 No.27 (Total No.140) Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the method for determination of hydrogen peroxide in bee pollen enzymes. This standard applies to the determination of catalase had in bee pollen. GB/T 23195-2008: Method for the determination of catalase in bee pollen -- Ultraviolet spectrophotometry---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Method for the determination of catalase in bee pollen. Ultaraviolet spectrophotometry ICS 65.140 B47 National Standards of People's Republic of China Determination of catalase bee pollen too UV spectrophotometry Posted 2008-12-31 2009-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordThis standard is proposed and managed by China Federation of Supply and Marketing Cooperatives. This standard was drafted. Guangzhou City Po Sang Park Co., Ltd., South China Agricultural University. The main drafters of this standard. Long Zheng Yao, Liu Wei, Chen Weibin. Determination of catalase bee pollen too UV spectrophotometry1 ScopeThis standard specifies the method for determination of bee pollen of catalase. This standard is applicable by measuring catalase in bee pollen.2 Normative referencesThe following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) Principle 3 Hydrogen peroxide absorbs strongly at a wavelength of 240nm, catalase can decompose hydrogen peroxide, the reaction solution in a certain range of absorbance Inner circle with the reaction time reduced, can be determined according to the rate of change in absorbance measured catalase activity.4 ReagentsUnless otherwise indicated in the analysis using only analytical reagent and in line with GB/T 6682 requirements distilled or deionized water. 4.1 phosphate buffer. pH 6.8 to 7.0, at a concentration of 50mmol/L, containing 1mmol/L disodium ethylenediamine tetraacetate (EDTA). Weigh accurately 17.91gNa2HPO4 · 12H2O add distilled water and diluted to 500mL, as solution A; Weigh accurately 7.80gNaH2PO4 · 2H2O add distilled water and diluted to 500mL, as solution B; The exact amount of fluid and take 55.0mLA 45.0mLB liquid in 200mL beaker, 0.07gEDTA, add distilled water mixed Even (added to about 150mL), calibrated to 6.8 to 7.0 with a pH meter, transferred to 200mL volumetric flask, dilute to volume, and mix. 4.2 hydrogen peroxide solution. volume fraction of 0.1%. Imbibe 0.5mL30% hydrogen peroxide is placed 50mL brown volumetric flask, with phosphate buffer (4.1) to volume, mix, Accurately taken from the triangular 10.0mL in 100mL Erlenmeyer flask (dark), and then accurately weighed 20.0mL phosphate buffer solution (4.1) was added to the mixed Even that was. 4.3 liquid nitrogen.5 Equipment5.1 UV spectrophotometer. 5.2 refrigerated centrifuge. 5.3 constant temperature water bath. 5.4 pH meter. 5.5 Analysis of balance. a sense of the amount of 0.1mg. 5.6 pipette. 100μL, 5000μL.6 Preparation of test solutionWeigh 1g sample to the nearest 1mg, has been placed with the right amount of liquid nitrogen ice-cold porcelain mortar while adding liquid nitrogen side grinding, the sample to be fully RESEARCH ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 23195-2008_English be delivered?Answer: Upon your order, we will start to translate GB/T 23195-2008_English as soon as possible, and keep you informed of the progress. 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