GB/T 21637-2008 English PDF

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GB/T 21637-2008: Method of morphological identification of coronavirus by using transmission electron microscopy
Status: Valid

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Basic data

Standard ID: GB/T 21637-2008 (GB/T21637-2008)
Description (Translated English): Method of morphological identification of coronavirus by using transmission electron microscopy
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: N53
Classification of International Standard: 11.100; 71.040.99
Word Count Estimation: 14,161
Date of Issue: 2008-04-11
Date of Implementation: 2008-10-01
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the application of transmission electron microscopy methods and identification of coronavirus, requirements and coronavirus morphology and morphogenesis feature. This standard applies to the use of transmission electron microscopy coronavirus morphological identification.

GB/T 21637-2008: Method of morphological identification of coronavirus by using transmission electron microscopy

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Method of morphological identification of coronavirus by using transmission electron microscopy ICS 11.100; 71.040.99 N53 National Standards of People's Republic of China Coronavirus transmission electron microscopy Morphological identification method Posted 2008-04-11 2008-10-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard and Appendix B is an informative annex. This standard by the National Standardization Technical Committee microbeam analysis and focal points. This standard was drafted. Institute of Geology and Geophysics, Chinese Academy of Military Medical Sciences Basic Medicine Institute, the National Center of Biomedical Analysis, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Second Military Medical University foundation Department of Fudan University Shanghai Medical College, Guangzhou Xing Shi-yuan Biological Technology Co., Ltd. Photonic Microsystems. The main drafters of this standard. Chen Dehui, Zeng Rong tree, Yang Yi, Liu Shide, Yong Ji, Yu Chang, Bi Shu.

Introduction

With the development of electron microscopy, and in vitro virus virus separation and purification technology, the virus morphology, chemical composition, genetic structure Increasing knowledge of biological characteristics as well as its other aspects. According to virus transmission electron microscope morphological characteristics. virion Shape and size, and the capsid symmetry (symmetry or helical symmetry is a perspective) and the size, number and arrangement of shell particles, in a host of fine nucleocapsid Intracellular replication and assembly sites, with or without an envelope, the envelope surface of the spike of the size, shape and spacing, and enveloped virus budding maturity site Etc., combined with the virus biology and molecular characterization of nucleic acids and proteins of the virus classification and nomenclature. Known people Class of pathogenic viruses involved in at least 22 families (Family) and two unclassified virus genus (Genus). Virus taxonomy and nomenclature Morphological characteristics of the virus is the primary indication. Practice has proved that, according to virus morphology and morphogenesis characteristics can be applied quasi-electron microscopy Correctly identified the virus to families. In order to properly guide diagnosis and appraisal of human viral infectious viral pathogens, it is necessary to develop human Disease-associated virus transmission electron microscope morphological analysis Identification of national standards. Coronavirus transmission electron microscopy Morphological identification method

1 Scope

This standard specifies the electron microscope observation and identification of coronavirus transmission method of application, requirements and coronavirus morphology and shape hair Health characteristics (see Appendix A, Appendix B). This standard applies to the use of a transmission electron microscope identification of the coronavirus morphology.

2 Terms and Definitions

The following terms and definitions apply to this standard. 2.1 A class of relatively primitive, with only one nucleic acid (DNA or RNA), capable of self-replication and cell parasitic within strict non-cellular organisms. 2.2 Copy, fully assembled mature viral particles. 2.3 Capsid proteins and nucleic acids by the composition of the center of the virus, there are two kinds of three-dimensional symmetry and helical symmetry. 2.4 Protein subunits are multiplexed nucleic acid wound on the outside, along the central axis was spirally arranged, the formation of highly ordered, symmetrical stable structure. 2.5 Outside the viral nucleocapsid by lipoprotein and mucin consisting of lipid bilayer membranes. 2.6 The outer envelope spike glycoprotein composed of projections having antigenicity. 2.7 High-density heavy metal salts (such as phosphotungstic acid, uranyl acetate, etc.) dye, will be surrounded by a tiny virus, the virus is displayed on a dark background Fine structure. 2.8 Ultra-thin slicer fixed biological sample after embedding, cut into thin slices having a thickness of 60nm ~ 80nm for TEM Observed.

3 equipment and materials

3.1 The main equipment a) transmission electron microscope; ......

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