GB/T 21033-2007 English PDFUS$159.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 21033-2007: Determination of immunoglobulin in feeds -- High-performance liquid chromatography Status: Valid
Basic dataStandard ID: GB/T 21033-2007 (GB/T21033-2007)Description (Translated English): Determination of immunoglobulin in feeds -- High-performance liquid chromatography Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B46 Classification of International Standard: 65.120 Word Count Estimation: 6,699 Date of Issue: 2007-06-21 Date of Implementation: 2007-09-01 Regulation (derived from): China National Standard Announcement 2007 No.7 (Total No.107) - National-Standard-Commission Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the method for the determination of IgG levels in feed using high performance liquid chromatography. This standard applies to feed, concentrated feed, feed ingredients containing IgG (including plasma protein powder) Determination of IgG. The minimum detection limit of 5 ��g (sampling 1g, the minimum detectable concentration of 5 mg/kg). GB/T 21033-2007: Determination of immunoglobulin in feeds -- High-performance liquid chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Determination of immunoglobulin in feeds. High-performance liquid chromatography ICS 65.120 B46 National Standards of People's Republic of China High Performance Liquid Chromatography Posted 2007-06-21 2007-09-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordThis standard by the National Standardization Technical Committee Feed Industry and focal points. This standard is drafted by. National Feed Quality Supervision and Inspection Center (Wuhan), Wuhan En Peter biotechnology limited liability company. The main drafters of this standard. Liu Xiaomin, Zhou increase too, He Yifan, Gregory red, Yang Lin. High Performance Liquid Chromatography1 ScopeThis standard specifies the method for the determination of IgG content in feed by high performance liquid chromatography. This standard applies to feed, concentrated feed and feed ingredients containing IgG (including plasma protein powder) of immunoglobulin IgG Determination. The minimum detection limit of 5μg (sample 1g, minimum detectable concentration of 5mg/kg).2 Normative referencesThe following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 14699.1 feed samples GB/T 20195 Animal feed sample preparation Principle 3 According to the principle of high-performance affinity chromatography, IgG immunoglobulin and ligand connected in phosphate buffer conditions, pH2.5 hydrochloric acid Elution of IgG glycine conditions.4 ReagentsUnless otherwise indicated in the analysis using only recognized as analytical reagent, the water is deionized water or equivalent purity, shall comply GB/T 6682 two water requirements. 4.1 potassium dihydrogen phosphate. 4.2 dipotassium hydrogen. 4.3 Mobile phase A. pH6.5,0.05mol/L phosphate buffer. 4.4 Mobile phase B. pH2.5,0.05mol/L glycine-HCl buffer. 4.5 IgG stock standard solution. Weigh IgG standard (purity ≥95%) 0.0100g, dissolved and set the volume to 10.0mL, shake, concentration of mobile phase A (4.3) 1.0mg/mL. 4.6 IgG working standard solution. Take IgG standard stock solution was diluted to a standard series containing IgG0.2,0.4,0.6,0.8,1.0mg/mL with mobile phase A (4.3). Pro formulated with time. 5. Apparatus 5.1 commonly used laboratory equipment. 5.2 pH meter. 5.3 ultra-pure water equipment. 5.4 homogenizer. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 21033-2007_English be delivered?Answer: Upon your order, we will start to translate GB/T 21033-2007_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 21033-2007_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 21033-2007_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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