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GB/T 20189-2025 English PDF

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GB/T 20189-2025: Determination of β-receptor agonists in feeds - Liquid chromatography-tandem mass spectrometry
Status: Valid

GB/T 20189: Historical versions

Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
GB/T 20189-2025249 Add to Cart 3 days Determination of β-receptor agonists in feeds - Liquid chromatography-tandem mass spectrometry Valid
GB/T 20189-2006139 Add to Cart 2 days Determination of ractopamine in animal feeds -- High performance liquid chromatographic method Valid

Similar standards

GB/T 19541   GB/T 18872   GB/T 18823   GB/T 20190   GB/T 20195   

Basic data

Standard ID: GB/T 20189-2025 (GB/T20189-2025)
Description (Translated English): Determination of ��-receptor agonists in feeds - Liquid chromatography-tandem mass spectrometry
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B46
Classification of International Standard: 65.120
Word Count Estimation: 12,121
Date of Issue: 2025-06-30
Date of Implementation: 2026-01-01
Older Standard (superseded by this standard): GB/T 22147-2008, GB/T 20189-2006
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 20189-2025: Determination of β-receptor agonists in feeds - Liquid chromatography-tandem mass spectrometry


---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT20189-2025
National Standard of the People's Republic of China ICS 65.120CCS B 46 Determination of β-receptor agonists in feed Liquid chromatography-tandem mass spectrometry Determination of β⁃receptor agonists in feeds- Liquid chromatography⁃tandem mass spectrometry Released on June 30, 2025 2026-01-01 implementation State Administration for Market Regulation The National Standardization Administration issued Replaces GB/T 22147-2008, GB/T 20189-2006

Preface

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents" Drafting is required. This document replaces GB/T 20189-2006 "Determination of ractopamine in feed - High performance liquid chromatography method" and GB/T 22147- 2008 "Determination of salbutamol, ractopamine and clenbuterol hydrochloride in feed by liquid chromatography-mass spectrometry", GB/T 20189- Compared with GB/T 22147-2008, in addition to structural adjustments and editorial changes, the main technical changes in.2006 are as follows. a) The scope of application, detection limit and quantification limit have been changed, and 21 β-receptor agonists have been added as test objects (see Chapter 1, GB/T 20189- 2006 and Chapter 1 of GB/T 22147-2008); b) The principles have been changed (see Chapter 4, Chapter 3 of GB/T 20189-2006 and GB/T 22147-2008); c) The reagents or materials have been changed (see Chapter 5, Chapter 4 of GB/T 20189-2006 and GB/T 22147-2008); d) The instruments and equipment have been changed (see Chapter 6, Chapter 5 of GB/T 20189-2006 and GB/T 22147-2008); e) The sample preparation method has been changed and sampling has been deleted (see Chapter 7, GB/T 20189-2006 and GB/T 22147-2008). Chapter 6); f) The test procedures have been changed (see Chapter 8, 7.1 of GB/T 20189-2006 and 7.1.1 of GB/T 22147-2008); g) The precision has been changed (see Chapter 10 of GB/T 20189-2006 and Chapter 9 of GB/T 22147-2008). Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents. This document is proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC 76). This document was drafted by. Sichuan Provincial Feed Work Station, Sichuan Well Testing Technology Co., Ltd., Agricultural Quality Control Center of the Chinese Academy of Agricultural Sciences Institute of Quality Standards and Testing Technology, Sichuan Agricultural Product Quality and Safety Center, Sichuan University. The main drafters of this document are. Zhao Lijun, Zhang Fengping, Fan Xia, Wu Yashan, Deng Pingling, Liu Wenbin, Zhang Jing, Suo Decheng, Yue Qin, Li Yun, Chen Hong, Ding Xiaoqiao, Li Qinrui, Wang Ying, Bian Zhengrong, and Chen Xiaochun. The previous versions of this document and the documents it replaces are as follows. --Published as GB/T 20189-2006 in.2006 and GB/T 22147-2008 in.2008; --This is the first revision. Determination of β-receptor agonists in feed Liquid chromatography-tandem mass spectrometry

1 Scope

This document describes a method for the determination of 21 β-agonists in feed by liquid chromatography-tandem mass spectrometry. This document applies to the use of bambuterol, fenoxypropionamide, carboplatin, and succinate in compound feeds, concentrates, concentrate supplements, and additive premixes. Butrol, Clenpent, Clenpro, Clenserol, Clenbuterol, Ractopamine, Ritodrine, Clorprenaline, Mabutrol, Mapentol Luo, hydroxymethyl clenbuterol, zilpaterol, salbutamol, terbutaline, tolbuterol, cibuterol, cimaterol, bromobuterol and bromo Determination of Clenbuterol. In this document, the detection limit of salbutamol, ractopamine, and clenbuterol was 5 μg/kg, and the quantification limit was 10 μg/kg; the The detection limit was 10 μg/kg and the quantification limit was 50 μg/kg.

2 Normative references

The contents of the following documents constitute the essential clauses of this document through normative references in this document. For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to This document. GB/T 6682 Specifications and test methods for water used in analytical laboratories GB/T 20195 Preparation of animal feed samples

3 Terms and Definitions

There are no terms or definitions that require definition in this document.

4 Principles

The analytes in the sample were extracted with hydrochloric acid-methanol solution, purified by solid phase extraction column or filter membrane, and determined by liquid chromatography-tandem mass spectrometry. Calibration was performed using a matching standard curve and quantification was performed using the external standard method.

5 Reagents or materials

Unless otherwise specified, only analytical grade reagents were used. 5.1 Water. GB/T 6682, Grade 1. 5.2 Methanol. chromatographic grade. 5.3 Acetonitrile. chromatographic grade. 5.4 Formic acid. chromatographic grade. 5.5 Hydrochloric acid solution (0.1 mol/L). Pipette 8.3 mL of hydrochloric acid, dilute with water to 1000 mL, and mix thoroughly. 5.6 Hydrochloric acid-methanol solution. Pipette 80 mL of hydrochloric acid solution (5.5), add 20 mL of methanol (5.2), and mix well. 5.7 5% ammonia methanol solution. Pipette 5 mL of ammonia and 95 mL of methanol (5.2) and mix well.
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