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GB/T 19539-2025 English PDF

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US$219.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 19539-2025: Determination of ochratoxin A in feeds
Status: Valid

GB/T 19539: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 19539-2025	219	Add to Cart	3 days	Determination of ochratoxin A in feeds	Valid
GB/T 19539-2004	189	Add to Cart	3 days	Determination of ochratoxin A in feeds	Valid

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Basic data

Standard ID: GB/T 19539-2025 (GB/T19539-2025)
Description (Translated English): Determination of ochratoxin A in feeds
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B46
Classification of International Standard: 65.120
Word Count Estimation: 10,138
Date of Issue: 2025-08-01
Date of Implementation: 2026-02-01
Older Standard (superseded by this standard): GB/T 19539-2004
Issuing agency(ies): State Administration for Market Regulation, Standardization Administration of China

GB/T 19539-2025: Determination of ochratoxin A in feeds

---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT19539-2025
ICS 65.120 CCSB46 National Standard of the People's Republic of China Replaces GB/T 19539-2004 Determination of ochratoxin A in feed Released on August 1, 2025 Implementation on February 1, 2026 State Administration for Market Regulation The National Standardization Administration issued

Preface

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents" Drafting. This document replaces GB/T 19539-2004 “Determination of ochratoxin A in feed”. In addition to structural adjustments and editorial changes, the main technical changes are as follows. a) The scope of application has been changed (see Chapter 1, Chapter 1 of the.2004 edition); b) Thin layer chromatography (see Chapter 3 of the.2004 edition) has been deleted; c) Enzyme-linked immunosorbent assay (ELISA) was deleted (see Chapter 4 of the.2004 edition); d) Liquid chromatography-tandem mass spectrometry has been added (see Chapter 4). Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents. This document is proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC76). This document was drafted by. Guangzhou Huibiao Testing Technology Center and National Animal Husbandry Station. The main drafters of this document are. Hao Yanjuan, Yang Yucheng, Zhao Enze, Wang Zhimin, Luo Zifeng, Su Shenglan, Liang Shuzhen, Lin Cuiwen, Luo Qun, Long Zhixi, Shen Darong, Ding Zhenhao, Pan Huanyu, Xie Shuyue, and He Qixia. The previous versions of this document and the documents it replaces are as follows. ---First issued in.2004 as GB/T 19539-2004; ---This is the first revision. Determination of ochratoxin A in feed

1 Scope

This document describes a method for the determination of ochratoxin A in feed by liquid chromatography-tandem mass spectrometry. This document applies to compound feeds, concentrated feeds, concentrate supplements, additive premixes, mixed feed additives and plant-based feeds. Determination of ochratoxin A in raw materials. The detection limit of this document is 0.2μg/kg and the quantification limit is 0.5μg/kg.

2 Normative references

The contents of the following documents constitute the essential clauses of this document through normative references in this document. For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to This document. GB/T 6682 Specifications and test methods for water used in analytical laboratories GB/T 20195 Preparation of animal feed samples

3 Terms and Definitions

There are no terms or definitions that require definition in this document.

4 Principles

Ochratoxin A in the sample was extracted with acetonitrile and water, cleaned up by immunoaffinity column, and detected by liquid chromatography-tandem mass spectrometry. Prepare standard solution for calibration and use external standard method for quantification.

5 Reagents or materials

warn. a) Any container that comes into contact with ochratoxin A must be immersed in a 4% sodium hypochlorite solution and cleaned after half a day for later use; b) For safety reasons, analysts must wear medical latex gloves when operating. Unless otherwise specified, only analytical grade reagents were used. 5.1 Water. GB/T 6682, Grade 1. 5.2 Methanol. Chromatographic grade. 5.3 Potassium hydroxide solution. Weigh 5.6 g of potassium hydroxide, dissolve it in water, and dilute to 100 mL with water. 5.4 Phosphate buffered saline (PBS). weigh 8.0 g sodium chloride, 1.2 g disodium hydrogen phosphate, 0.2 g potassium dihydrogen phosphate, and 0.2 g potassium chloride and dissolve them in Add 900 mL of water, adjust the pH to 7.0 with potassium hydroxide solution (5.3), make up to 1 L with water, and mix well. 5.5 Extraction solution. Measure 800 mL of acetonitrile and.200 mL of water and mix well. 5.6 Eluent. Take 2 mL of acetic acid, dilute to 100 mL with methanol, and mix thoroughly. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 19539-2025_English be delivered?

Answer: Upon your order, we will start to translate GB/T 19539-2025_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 19539-2025_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 19539-2025_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.

Question 5: Should I purchase the latest version GB/T 19539-2025?

Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB/T 19539-2025 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.