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GB/T 19495.5-2018 English PDF

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US$439.00 · In stock
Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 19495.5-2018: Detection of genetically modified organisms and derived products -- Quantitative real-time polymerase chain reaction (PCR) methods
Status: Valid

GB/T 19495.5: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 19495.5-2018	439	Add to Cart	4 days	Detection of genetically modified organisms and derived products -- Quantitative real-time polymerase chain reaction (PCR) methods	Valid
GB/T 19495.5-2004	RFQ	ASK	6 days	Detection of genetically modified organisms and derived products -- Quantitative nucleic acid-based methods	Obsolete

Similar standards

GB/T 43165 GB/T 40135 GB/T 19495.4 GB/T 28108 GB/T 31790 GB/T 19495.9

Basic data

Standard ID: GB/T 19495.5-2018 (GB/T19495.5-2018)
Description (Translated English): Detection of genetically modified organisms and derived products -- Quantitative real-time polymerase chain reaction (PCR) methods
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 22,262
Date of Issue: 2018-09-17
Date of Implementation: 2019-04-01
Older Standard (superseded by this standard): GB/T 19495.5-2004
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 19495.5-2018: Detection of genetically modified organisms and derived products -- Quantitative real-time polymerase chain reaction (PCR) methods

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of genetically modified organisms and derived products - Quantitative real-time polymerase chain reaction (PCR) methods ICS 65.020.01 B16 National Standards of People's Republic of China Replace GB/T 19495.5-2004 Genetically modified product testing Real-time fluorescence quantitative polymerase chain reaction (PCR) Detection method Published on.2018-09-17 Implementation of.2019-04-01 State market supervision and administration China National Standardization Administration issued

Foreword

GB/T 19495 "GMO Testing" is divided into the following sections. ---GB/T 19495.1 General requirements and definitions for the detection of genetically modified products; ---GB/T 19495.2 Technical requirements for testing laboratory of genetically modified products; ---GB/T 19495.3 Detection of nucleic acid extraction and purification methods for transgenic products; ---GB/T 19495.4 Transgenic product detection real-time fluorescence qualitative polymerase chain reaction (PCR) detection method; ---GB/T 19495.5 Transgenic product detection real-time fluorescence quantitative polymerase chain reaction (PCR) detection method; ---GB/T 19495.6 Genetically modified product detection gene chip detection method; ---GB/T 19495.7 GM product testing sampling and sample preparation methods; ---GB/T 19495.8 Detection method for protein detection of genetically modified products; ---GB/T 19495.9 Detection method of liquid product chip for detection of plant products by genetically modified products. This part is the fifth part of GB/T 19495. This part is drafted in accordance with the rules given in GB/T 1.1-2009. This part replaces GB/T 19495.5-2004 "Quantitative PCR detection method for detection of nucleic acid in genetically modified products". And GB/T 19495.5- Compared with.2004, the main technical changes except editorial changes are as follows. --- Revised the scope of application of the standard; --- Added the ability to quantitatively detect transgenic plant lines in samples based on matrix reference materials and plasmid standard molecules, respectively. Procedure --- Increased the copy number of transgenic plant lines such as soybean, corn, canola, cotton, rice, potato, sugar beet and papaya Quantitative detection method. This part is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This section drafted by. China Academy of Inspection and Quarantine, Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, the Chinese people Heguo Shandong Entry-Exit Inspection and Quarantine Bureau and Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this section. Huang Xin, Li Xiang, Gao Hongwei, Ling Xingyuan, Zhu Shuifang, Chen Hongjun, Pan Liangwen, Cao Jijuan, Zhang Guiming. The previous versions of the standards replaced by this section are. ---GB/T 19495.5-2004. Genetically modified product testing Real-time fluorescence quantitative polymerase chain reaction (PCR) Detection method

1 Scope

This part of GB/T 19495 specifies soybeans, corn, rapeseed, rice (rice), cotton, potatoes, beets, papaya and other plants and A real-time fluorescent PCR quantitative detection method for the content of transgenic lines in products. This section applies to quantitative detection methods for hundreds of copies of transgenic lines in the above plants and their products.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 19495.2 Technical requirements for testing laboratory of genetically modified products GB/T 19495.3 Transgenic product detection nucleic acid extraction and purification method GB/T 19495.7 GM product testing sampling and sample preparation method GB/T 27403 Laboratory Quality Control Specification for Food Molecular Biology Testing JJF1059.1 Measurement Uncertainty Evaluation and Representation SN/T 4562 Transgenic Testing Laboratory Measurement Uncertainty Evaluation Guide 3 Terms, definitions and abbreviations 3.1 Terms and definitions The following terms and definitions apply to this document. 3.1.1 Transgenic transgene Functional DNA sequences derived from other species that are not native to the species, through bioengineering techniques, allowing them to enter the species Lines are expressed in order to give the species the technology to acquire new traits. 3.1.2 Line-specific event-specific The contiguous region sequence resulting from the recombination of the foreign DNA into the recipient crop genome. 3.1.3 Real-time fluorescence PCR real-timepolymerasechainreaction Adding a fluorophore to the polymerase chain reaction system, monitoring the entire PCR process in real time using fluorescence signal accumulation, and passing the standard A method of quantitative analysis of an unknown template by a curve. 3.1.4 Internal standard gene endogenousreferencegene A gene with a constant copy number and no display of allelic changes in the species is detected. This gene can be used to determine species specificity. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 19495.5-2018_English be delivered?

Answer: Upon your order, we will start to translate GB/T 19495.5-2018_English as soon as possible, and keep you informed of the progress. The lead time is typically 2 ~ 4 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 19495.5-2018_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 19495.5-2018_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.

Question 5: Should I purchase the latest version GB/T 19495.5-2018?

Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB/T 19495.5-2018 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.