GB/T 18869-2019 English PDF

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GB/T 18869-2019: Determination of coliforms in feeds
Status: Valid

GB/T 18869: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 18869-2019	359	Add to Cart	4 days	Determination of coliforms in feeds	Valid
GB/T 18869-2002	399	Add to Cart	3 days	Determination of coliform bacteria in feeds	Obsolete

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Basic data

Standard ID: GB/T 18869-2019 (GB/T18869-2019)
Description (Translated English): Determination of coliforms in feeds
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B46
Classification of International Standard: 65.120
Word Count Estimation: 18,145
Date of Issue: 2019-06-04
Date of Implementation: 2020-01-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 18869-2019: Determination of coliforms in feeds

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of coliforms in feeds ICS 65.120 B46 National Standards of People's Republic of China Replace GB/T 18869-2002 Determination of coliforms in feed Published on.2019-06-04 2020-01-01 implementation State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard replaces GB/T 18869-2002 "Determination of coliforms in feed". Compared with GB/T 18869-2002, the main technical contents of this standard are revised as follows. --- Revised the scope of application (see Chapter 1, Chapter 1 of the.2002 edition); --- Added terms and definitions (see Chapter 3); --- Modified the description of the principle (see Chapter 4, Chapter 3 of the.2002 edition); --- Increased LST fermentation (see 8.3). This standard is proposed and managed by the National Feed Industry Standardization Technical Committee (SAC/TC76). This standard was drafted. Institute of Agricultural Quality Standards and Testing Technology, Chinese Academy of Agricultural Sciences [National Feed Quality Supervision and Inspection Center (Beijing)]. The main drafters of this standard. Li Lizhen, Liu Xiaolu, Li Sheng, Wang Wei. The previous versions of this standard were released as follows. ---GB/T 18869-2002. Determination of coliforms in feed

1 Scope

This standard specifies the method for determination of coliforms in feed. This standard applies to the determination of coliforms in feed and feed additives.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Terms and definitions

The following definitions apply to this document. 3.1 Coliforms coliforms Aerobic or facultative anaerobic Gram-negative Bacillus-free bacilli capable of fermenting lactose to produce acid gas under certain culture conditions. 3.2 Most likely number of mostprobablenumber;MPN An indirect counting method based on Poisson distribution.

4 Principle

After the sample to be tested is diluted to an appropriate concentration and cultured, the statistics are applied according to the lowest dilution of the growth and the highest dilution of growth. Learn the probability theory to calculate the maximum possible number of coliforms in the sample to be tested.

5 medium or material

Only reagents identified as analytically pure were used in the analysis unless otherwise stated. 5.1 Water. Meet the requirements of the third grade water in GB/T 6682. 5.2 Lactose bile salt fermentation medium. See Appendix A, A.1. 5.3 Ivy Blue Agar. See Appendix A, A.2. 5.4 Lactose fermentation medium. See Appendix A, A.3. 5.5 Gram stain. See Appendix A, A.4. 5.6 lauryl sulfate tryptone (LST) broth. see Appendix A, A.5. 5.7 Brilliantgreen lactosebile (BGLB) broth. See Appendix A, A.6. 5.8 Phosphate buffer. See A.7 in Appendix A. 5.9 Sterile saline. See Appendix A, A.8. ......

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