GB/T 18089-2008 English PDF

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GB/T 18089-2008: Isolation, identification and serum neutralization antibody test in bluetongue virus
Status: Valid

GB/T 18089: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 18089-2008	229	Add to Cart	3 days	Isolation, identification and serum neutralization antibody test in bluetongue virus	Valid
GB/T 18089-2000	359	Add to Cart	3 days	Micro-serum neutralization test, virus isolation and identification for bluetongue	Obsolete

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GB/T 25165   NY/T 1663   GB/T 20365   GB/T 26436   GB/T 18936   GB/T 18090   

Basic data

Standard ID: GB/T 18089-2008 (GB/T18089-2008)
Description (Translated English): Isolation, identification and serum neutralization antibody test in bluetongue virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 10,172
Date of Issue: 2008-12-31
Date of Implementation: 2009-05-01
Older Standard (superseded by this standard): GB/T 18089-2000
Regulation (derived from): National Standard Approval Announcement 2008 No.25 (Total No.138)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the bluetongue virus isolation, identification and serum neutralizing antibody detection techniques. This standard applies to bluetongue virus isolation, identification and animal infection bluetongue virus antibodies in serum and detection.

GB/T 18089-2008: Isolation, identification and serum neutralization antibody test in bluetongue virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Isolation, identification and serum neutralization antibody test in bluetongue virus ICS 11.220 B41 National Standards of People's Republic of China Replacing GB/T 18089-2000 Bluetongue virus isolation, identification and serum and Antibody detection technology Posted 2008-12-31 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard replaces GB/T 18089-2000 "Bluetongue micro serum neutralization test and the isolation and identification of the virus." This standard and GB/T 18089-2000 compared to the main changes are as follows. --- Standard name has been modified; --- Remove the bluetongue virus identified in a virus neutralization test portion; --- Increase in the identification of viral identification RT-PCR method. Appendix A of this standard is a normative appendix. The standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Standardization Technical Committee on Animal Epidemic Prevention. This standard was drafted. People's Republic of China Yunnan Exit Inspection and Quarantine, Shenzhen People's Republic of China Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Zhou Xiaoli, Yang Jianming, Ai Jun, flower Qun Yi, Yang flower, Zhangqi Yan, Yan tree hair, Dong Jun, Ye Lingling Xu Vega. This standard replaces the standards previously issued as follows. --- GB/T 18089-2000. Bluetongue virus isolation, identification and serum and Antibody detection technology

1 Scope

This standard specifies the bluetongue virus isolation, identification and serum neutralizing antibody detection techniques. This standard applies to the bluetongue virus isolation, identification and animal infected with bluetongue virus serum neutralizing antibody test.

2 Symbols and Abbreviations

The following symbols and abbreviations apply to this standard. BHK21 --- baby hamster kidney cell line; BLP --- buffered lactose peptone broth; BT --- bluetongue; bp --- base pairs; CPE --- induced cytopathic effect; C6/36 --- mosquito cell lines; --- Of DEPC diethylpyrocarbonate; SPF --- specific pathogen; TCID50 --- median tissue culture infective dose; Vero --- African green monkey kidney cell line.

3 bluetongue virus isolation

3.1 Materials 10 days to 11-day-old SPF chicken embryos. 3.2 Equipment and Apparatus Chick opening, Egg or Egg lamp box, a mortar or tissue masher, inverted microscope. 3.3 Test Procedure 3.3.1 Sample Anticoagulant heparin (10IU per 5mL blood with heparin), organs (spleen, liver, kidney, lymph nodes), semen, Culicoides. For virus isolation Samples should be stored in the refrigerated container home, sent to the laboratory within 24h, and processed immediately. 3.3.2 Blood Sample Preparation Take anticoagulant heparin 5mL, with phosphate buffered saline (PBS, recipe see Appendix A) Blood samples were washed 3 to 4 times, each time 2000r/min Centrifugal 10min, the supernatant was added after the times of BLP (recipe see Appendix A), set in an ice bath with sonication (40μA, 1min). By the Department Reasonable sample that day, saved using the pre-4 ℃, -70 ℃ to save the remaining sample set aside. 3.3.3 Preparation of tissue samples Collected aseptically animal spleen, lymph nodes, liver, kidney each 5g, cut into pieces added BLP10mL (including penicillin 2000IU/mL, streptomycin 2000μg/mL), set Mortar grinding, 4 ℃ refrigerator extraction 4h, the supernatant 5mL treated with sonication (100μA, 1min ~ 2min) or freezing and thawing three times, 3000r/min centrifugal 20min, supernatant using, use the pre-stored at 4 ℃, -70 ℃ remaining sample set Paul Deposit reserve. 3.3.4 semen sample preparation After taking a sperm sample 0.5mL, with BLP as 1.5 dilution, 3000r/min centrifugal 20min, supernatant was discarded, and then make BLP ......

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